Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,993,949	T→C	D253G (GAC→GGC)	uvrC ←	excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,993,949	0	T	C	100.0%	215.9 / NA	60	D253G (GAC→GGC)	uvrC	excinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/30);  total (30/30)

CCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGC  >  NC_000913/1993824‑1994083                                                                                                                              |                                                                                                                                       ccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                           <  1:56740/139‑1 (MQ=255)                tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgtc                                                                                                                              >  2:975409/1‑121 (MQ=255)                tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgtc                                                                                                                              <  1:975409/121‑1 (MQ=255)                tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            >  1:1295677/1‑139 (MQ=255)                tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            >  1:1363644/1‑139 (MQ=255)                tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            <  1:40356/139‑1 (MQ=255)                     ccAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGtt                                                                                                       >  2:2483/1‑139 (MQ=255)                     ccAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGtt                                                                                                       >  2:1410607/1‑139 (MQ=255)                        ggCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGttttt                                                                                                    <  2:926327/139‑1 (MQ=255)                          cACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCg                                                                                                  >  1:859633/1‑139 (MQ=255)                             tttCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                               <  2:859633/139‑1 (MQ=255)                                      aTAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGc                                                                                      <  1:2483/139‑1 (MQ=255)                                       tAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                     >  1:289794/1‑139 (MQ=255)                                             cGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCt                                                                               >  1:734680/1‑139 (MQ=255)                                                       gCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTc                                                                     <  2:103619/139‑1 (MQ=255)                                                          cTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            >  2:308113/1‑97 (MQ=255)                                                          cTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            <  1:308113/97‑1 (MQ=255)                                                                    aCGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgt                                                                                                                               <  1:102591/68‑1 (MQ=255)                                                                    aCGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgt                                                                                                                               >  2:102591/1‑68 (MQ=255)                                                                     cGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAg                                                       <  2:1160046/139‑1 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                           >  1:94573/1‑55 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                           <  2:94573/55‑1 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                               >  2:1095245/1‑83 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                               <  2:493511/83‑1 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                               <  1:1095245/83‑1 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTg                                                                                               >  1:493511/1‑83 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                       >  1:7690/1‑139 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                       >  2:1538583/1‑139 (MQ=255)                                                                                     ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                       >  1:579031/1‑139 (MQ=255)                                                                                        cacaAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGc                                                                                 <  1:423770/94‑1 (MQ=255)                                                                                        cacaAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGc                                                                                 >  2:423770/1‑94 (MQ=255)                                                                                        cacaAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAtt                                    >  1:127166/1‑139 (MQ=255)                                                                                        cacaAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAtt                                    >  1:1630131/1‑139 (MQ=255)                                                                                          caAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTct                                  >  2:700105/1‑139 (MQ=255)                                                                                          caAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTct                                  >  2:1367410/1‑139 (MQ=255)                                                                                           aaGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctg                                 >  2:978760/1‑139 (MQ=255)                                                                                            aGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctgg                                >  1:116766/1‑139 (MQ=255)                                                                                                aTGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctgg                            <  2:1630131/139‑1 (MQ=255)                                                                                                aTGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctgg                            >  1:33997/1‑139 (MQ=255)                                                                                                    ccGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGt                        <  1:83450/139‑1 (MQ=255)                                                                                                    ccGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGt                        <  1:1102826/139‑1 (MQ=255)                                                                                                       cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            <  1:216991/52‑1 (MQ=255)                                                                                                       cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                            >  2:216991/1‑52 (MQ=255)                                                                                                       cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                     <  2:1418297/75‑1 (MQ=255)                                                                                                       cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                     >  1:1418297/1‑75 (MQ=255)                                                                                                        aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                    >  1:1548996/1‑139 (MQ=255)                                                                                                            aaCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGc                                                                                 <  1:1444673/74‑1 (MQ=255)                                                                                                            aaCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGc                                                                                 >  2:1444673/1‑74 (MQ=255)                                                                                                            aaCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATAc                <  1:700105/139‑1 (MQ=255)                                                                                                              cGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGa              <  2:7690/139‑1 (MQ=255)                                                                                                                ccACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACt            <  2:1548996/139‑1 (MQ=255)                                                                                                                 cacaCCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTa           <  1:1367410/139‑1 (MQ=255)                                                                                                                   cacCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCa                   <  1:1477880/129‑1 (MQ=255)                                                                                                                   cacCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCa                   >  2:1477880/1‑129 (MQ=255)                                                                                                                     ccAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGa       <  2:579031/139‑1 (MQ=255)                                                                                                                        aTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGtt    <  2:116766/139‑1 (MQ=255)                                                                                                                        aTAACGCCGAGGTCGTCGCCAGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGtt    <  2:734680/139‑1 (MQ=255)                                                                                                                          aaCGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGc  <  2:1363644/139‑1 (MQ=255)                                                                                                                           aCGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGc                                                      <  2:329845/86‑1 (MQ=255)                                                                                                                           aCGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGc                                                      >  1:329845/1‑86 (MQ=255)                                                                                                                              |                                                                                                                                       CCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGC  >  NC_000913/1993824‑1994083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑