Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,620 G→A *386* (TAG→TAA cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,6200GA100.0% 239.0 / NA 73*386* (TAG→TAAcdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/28);  total (45/28)

AAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183485‑183755
                                                                                                                                       |                                                                                                                                       
aaaGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     <  1:190052/139‑1 (MQ=255)
       ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTa                                                                                                                              >  2:907286/1‑139 (MQ=255)
                   cGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     >  1:408719/1‑120 (MQ=255)
                   cGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     <  2:408719/120‑1 (MQ=255)
                    gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcggc                                                                                                                 >  1:206014/1‑139 (MQ=255)
                              ggAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       >  1:147849/1‑139 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCACTTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   >  2:1170174/1‑137 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   <  1:1170174/137‑1 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  2:216620/1‑139 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:209304/1‑139 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  2:769836/1‑139 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:120089/1‑139 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:749556/1‑139 (MQ=255)
                                    tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  2:1542649/1‑139 (MQ=255)
                                     cGGCTTAATCGTATATCGGAACTGACCGAGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTACGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTACCCTc                                                                                                >  1:1026922/1‑139 (MQ=255)
                                         ttAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGcc                                                                                            <  1:216620/139‑1 (MQ=255)
                                            aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    >  2:1151544/1‑112 (MQ=255)
                                            aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    <  1:1151544/112‑1 (MQ=255)
                                             tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  2:749556/139‑1 (MQ=255)
                                                       gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                                              >  1:71351/1‑139 (MQ=255)
                                                        aaCTGACCGGGCTTGATTTGGGCAATTTGGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAgggg                                                                             <  2:1026922/139‑1 (MQ=255)
                                                          cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                                           >  2:415301/1‑139 (MQ=255)
                                                          cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                                           >  2:287781/1‑139 (MQ=255)
                                                          cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                                           >  2:994016/1‑139 (MQ=255)
                                                             aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:1572310/1‑139 (MQ=255)
                                                             aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:848067/1‑139 (MQ=255)
                                                             aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  2:1280240/1‑139 (MQ=255)
                                                                            ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTcccc                                                                                                  <  2:695765/98‑1 (MQ=255)
                                                                            ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTcccc                                                                                                  >  1:695765/1‑98 (MQ=255)
                                                                            ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                         >  1:250354/1‑139 (MQ=255)
                                                                                    tgatgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                                 <  1:994016/139‑1 (MQ=255)
                                                                                           aGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                >  1:473451/1‑69 (MQ=255)
                                                                                           aGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                <  2:473451/69‑1 (MQ=255)
                                                                                           aGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTa                                          >  1:974523/1‑139 (MQ=255)
                                                                                           aGGTTGCTGATGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAGCGTCAGTTCGGGATTATTTa                                          <  2:974523/139‑1 (MQ=255)
                                                                                            ggTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  2:191793/121‑1 (MQ=255)
                                                                                            ggTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           >  1:191793/1‑121 (MQ=255)
                                                                                                   gctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAg                                  <  2:1572310/139‑1 (MQ=255)
                                                                                                    ctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGt                                 <  1:952327/139‑1 (MQ=255)
                                                                                                       tATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                              >  1:1526157/1‑139 (MQ=255)
                                                                                                         tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                                             <  2:1063272/74‑1 (MQ=255)
                                                                                                         tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                                             >  1:1063272/1‑74 (MQ=255)
                                                                                                            ggCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                                 <  2:874876/115‑1 (MQ=255)
                                                                                                            ggCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                                 >  1:874876/1‑115 (MQ=255)
                                                                                                            ggCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAg                         >  2:373386/1‑139 (MQ=255)
                                                                                                              cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    <  2:518039/46‑1 (MQ=255)
                                                                                                              cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    >  1:518039/1‑46 (MQ=255)
                                                                                                              cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                                     >  2:588057/1‑45 (MQ=255)
                                                                                                              cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                                     <  1:588057/45‑1 (MQ=255)
                                                                                                              cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                       >  2:392275/1‑139 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                <  2:722640/47‑1 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                                >  1:722640/1‑47 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          <  2:1525066/69‑1 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          >  1:1525066/1‑69 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        >  2:631322/1‑71 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:631322/71‑1 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  1:1211613/1‑139 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  2:772552/1‑139 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  2:1541294/1‑139 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGCTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  1:1412021/1‑139 (MQ=255)
                                                                                                                         ggtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTg                                                                                                             >  1:1255579/3‑43 (MQ=255)
                                                                                                                         ggtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTg                                                                                                             <  2:1255579/41‑1 (MQ=255)
                                                                                                                         ggtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAt             <  1:1280240/137‑1 (MQ=255)
                                                                                                                          gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                      <  2:1523421/96‑1 (MQ=255)
                                                                                                                          gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                      >  1:1523421/2‑97 (MQ=255)
                                                                                                                             aaGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCg         <  1:907286/139‑1 (MQ=255)
                                                                                                                              agagCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGc        <  2:120089/139‑1 (MQ=255)
                                                                                                                                agCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                                      <  2:842647/107‑1 (MQ=255)
                                                                                                                                agCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                                      >  1:842647/1‑107 (MQ=255)
                                                                                                                                agCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTg      >  1:752019/1‑139 (MQ=255)
                                                                                                                                  cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                   >  2:601937/1‑60 (MQ=255)
                                                                                                                                  cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                   <  1:601937/60‑1 (MQ=255)
                                                                                                                                    gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCt  >  1:49682/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183485‑183755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: