Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,494	C→T	V6I (GTC→ATC)  *197* (TAG→TAA)	wbbK ← wbbJ ←	lipopolysaccharide biosynthesis protein putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,494	0	C	T	100.0%	169.9 / NA	53	V6I (GTC→ATC)  *197* (TAG→TAA)	wbbK wbbJ	lipopolysaccharide biosynthesis protein putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/28);  total (25/28)

CTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCATTACC  >  NC_000913/2104361‑2104625                                                                                                                                      |                                                                                                                                    ctttctttTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGc                                                                                                                                >  2:1423201/1‑139 (MQ=255)    tctttTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCttt                                                                                                                             >  1:23015/1‑139 (MQ=255)     ctttTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCtttt                                                                                                                            <  2:1245027/139‑1 (MQ=255)           aCTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCAt                                                                                                                      >  2:946293/1‑139 (MQ=255)              ctTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                                   <  1:33058/139‑1 (MQ=255)              ctTTAGCAGAATGGACTAATGAGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                                   <  2:970057/139‑1 (MQ=255)                    cAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtc                                                                                                             <  2:1392769/139‑1 (MQ=255)                     aGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                            <  1:895817/139‑1 (MQ=255)                         tGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATg                                                                                                        >  1:1469121/1‑139 (MQ=255)                         tGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATg                                                                                                        >  1:643432/1‑139 (MQ=255)                                              aCATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                              <  1:575614/112‑1 (MQ=255)                                              aCATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                              >  2:575614/1‑112 (MQ=255)                                               cATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGAttttt                                                                                  <  1:946293/139‑1 (MQ=255)                                                aTTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAGTGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       <  2:1186575/117‑1 (MQ=255)                                                aTTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       >  1:1186575/1‑117 (MQ=255)                                                          ttattaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                             <  1:1415677/133‑1 (MQ=255)                                                          ttattaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                             >  2:1415677/1‑133 (MQ=255)                                                               aGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       <  1:152288/102‑1 (MQ=255)                                                               aGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       >  2:152288/1‑102 (MQ=255)                                                               aGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTcc                                                                       <  2:1480804/134‑1 (MQ=255)                                                               aGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTcc                                                                       >  1:1480804/1‑134 (MQ=255)                                                                  tgctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGa                                                               <  1:1423201/139‑1 (MQ=255)                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                                  >  1:79304/1‑102 (MQ=255)                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                                  <  2:79304/102‑1 (MQ=255)                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                         >  1:217635/1‑127 (MQ=255)                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                         <  2:217635/127‑1 (MQ=255)                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                             >  1:399702/1‑139 (MQ=255)                                                                     tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAATATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTgg                                                                                                               <  1:1107183/88‑1 (MQ=255)                                                                     tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTgg                                                                                                               >  2:1107183/1‑88 (MQ=255)                                                                       ccAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTa                                                          <  2:1334517/139‑1 (MQ=255)                                                                             aTTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCg                                                    <  1:601227/139‑1 (MQ=255)                                                                             aTTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCg                                                    <  2:23015/139‑1 (MQ=255)                                                                             aTTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCg                                                    <  2:481744/139‑1 (MQ=255)                                                                                   tCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATa                                              <  2:643432/139‑1 (MQ=255)                                                                                    cAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAg                                             <  2:399702/139‑1 (MQ=255)                                                                                            tAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTa                                     >  1:1022585/1‑139 (MQ=255)                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTa                                                                                          <  1:1478924/79‑1 (MQ=255)                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTa                                                                                          >  2:1478924/1‑79 (MQ=255)                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAg                              >  1:1097446/1‑139 (MQ=255)                                                                                                      gCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTcc                                                                                                                         <  2:1431776/45‑1 (MQ=255)                                                                                                      gCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTcc                                                                                                                         >  1:1431776/1‑45 (MQ=255)                                                                                                          gTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                         >  2:60654/1‑89 (MQ=255)                                                                                                          gTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                         <  1:60654/89‑1 (MQ=255)                                                                                                            ggTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCt                                                                               <  1:1155071/81‑1 (MQ=255)                                                                                                            ggTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCt                                                                               >  2:1155071/1‑81 (MQ=255)                                                                                                                 aaTTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACt                <  2:1097446/139‑1 (MQ=255)                                                                                                                      aCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGAc           >  1:746106/1‑139 (MQ=255)                                                                                                                       ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       <  2:1189540/46‑1 (MQ=255)                                                                                                                       ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       >  1:1189540/1‑46 (MQ=255)                                                                                                                       ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACt          >  2:1616121/1‑139 (MQ=255)                                                                                                                       ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACt          >  1:780219/1‑139 (MQ=255)                                                                                                                       ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACt          >  1:12190/1‑139 (MQ=255)                                                                                                                               acaacGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCATTAcc  <  1:1150098/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    CTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCATTACC  >  NC_000913/2104361‑2104625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑