Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,274,166 A→G K601K (AAA→AAG)  yejA → microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,1660AG100.0% 204.5 / NA 58K601K (AAA→AAG) yejAmicrocin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/25);  total (33/25)

GTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGC  >  NC_000913/2274034‑2274297
                                                                                                                                    |                                                                                                                                   
gTACATGGCGGAAGACAGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                               >  1:1374121/1‑139 (MQ=255)
        cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                       >  2:220194/1‑136 (MQ=255)
           aaGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                    <  1:743915/139‑1 (MQ=255)
                          ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                                 <  2:1039168/111‑1 (MQ=255)
                          ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                                 >  1:1039168/1‑111 (MQ=255)
                           gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATgg                                                                                                                     <  2:691588/122‑1 (MQ=255)
                           gtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATgg                                                                                                                     >  1:691588/1‑122 (MQ=255)
                                     ttCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGc                                                                                          >  2:1352331/1‑139 (MQ=255)
                                         cccAGCCGGCCGTGTGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      >  1:533597/1‑139 (MQ=255)
                                          ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                                                                     >  2:833379/1‑139 (MQ=255)
                                            aGCCGGCCGTGTGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGAt                                                                                   <  2:533597/139‑1 (MQ=255)
                                               cggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                <  2:1202528/139‑1 (MQ=255)
                                                          cccATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                                     <  2:12159/139‑1 (MQ=255)
                                                                 ataGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                                              >  1:1371364/1‑139 (MQ=255)
                                                                   aGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCa                                                            <  1:833379/139‑1 (MQ=255)
                                                                    gCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                                  >  1:830923/1‑100 (MQ=255)
                                                                    gCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                                  <  2:830923/100‑1 (MQ=255)
                                                                     ccTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACAtt                                                                          >  1:726920/1‑123 (MQ=255)
                                                                     ccTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACAtt                                                                          <  2:726920/123‑1 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                                        <  1:220618/106‑1 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTg                                                                                        >  2:220618/1‑106 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      <  1:890308/108‑1 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      >  2:890308/1‑108 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                       >  2:1108978/1‑139 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                       >  1:484191/1‑139 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                       >  1:1227890/1‑139 (MQ=255)
                                                                        cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                       >  2:1434049/1‑139 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCGGGGAGAGTaaa                                                                                                                        >  2:1068837/1‑71 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAggg                                                                                                                                <  1:921275/65‑1 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaa                                                                                                                        <  1:1068837/73‑3 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaa                                                                                                                        >  1:748375/1‑71 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaa                                                                                                                        <  2:748375/73‑3 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                    <  1:390198/77‑1 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                    >  2:390198/1‑77 (MQ=255)
                                                                         ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                                      >  2:479764/1‑139 (MQ=255)
                                                                         ggTATCGATACCTGGTGGAATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAggg                                                                                                                                >  2:921275/1‑65 (MQ=255)
                                                                            aTCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACt                                                   >  2:341829/1‑139 (MQ=255)
                                                                                aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACttttt                                               >  1:594761/1‑139 (MQ=255)
                                                                                     tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCagcaagca                                                                                                                                   >  1:812924/1‑50 (MQ=255)
                                                                                     tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCagcaagca                                                                                                                                   <  2:812924/50‑1 (MQ=255)
                                                                                     tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcg                                                                                                                  >  2:1361072/1‑67 (MQ=255)
                                                                                     tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGgcg                                                                                                                  <  1:1361072/67‑1 (MQ=255)
                                                                                           tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCagcaagca                                                                                                                                   <  2:1051986/44‑1 (MQ=255)
                                                                                           tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      >  2:970452/1‑89 (MQ=255)
                                                                                           tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                      <  1:970452/89‑1 (MQ=255)
                                                                                                 gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGc                                                                                          >  1:181328/1‑79 (MQ=255)
                                                                                                 gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGc                                                                                          <  2:181328/79‑1 (MQ=255)
                                                                                                 gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGCGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATACCAACATTATGGGCGATTATCACCATCAAATTTTTCATCGTGCAAATTgcgc                              >  2:563076/1‑139 (MQ=255)
                                                                                                  tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGcc                             >  2:768779/1‑139 (MQ=255)
                                                                                                     aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                                              >  1:1503559/1‑103 (MQ=255)
                                                                                                     aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTAt                                                              <  2:1503559/103‑1 (MQ=255)
                                                                                                     aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTgg                          >  1:1404759/1‑139 (MQ=255)
                                                                                                             gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGc                                                                                                    >  2:1234680/1‑57 (MQ=255)
                                                                                                             gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGc                                                                                                    <  1:1234680/57‑1 (MQ=255)
                                                                                                             gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg                  >  1:1593900/1‑139 (MQ=255)
                                                                                                              ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                <  2:1247172/76‑1 (MQ=255)
                                                                                                              ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                >  1:1247172/1‑76 (MQ=255)
                                                                                                                          ccGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCAt     <  2:1404759/139‑1 (MQ=255)
                                                                                                                            gCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTCATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCAGCGTGCAAAGTGCGCCTGGCGGTCCGGTCGACCAGGCCATcg   <  2:1374121/139‑1 (MQ=255)
                                                                                                                             ccAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATcgc  <  1:220194/139‑1 (MQ=255)
                                                                                                                             ccAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATcgc  <  1:1352331/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGC  >  NC_000913/2274034‑2274297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑