Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,699	T→C	intergenic (+27/‑48)	ackA → / → pta	acetate kinase A and propionate kinase 2/phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,699	0	T	C	100.0%	221.5 / NA	61	intergenic (+27/‑48)	ackA/pta	acetate kinase A and propionate kinase 2/phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/34);  total (27/34)

TTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCC  >  NC_000913/2414565‑2414807                                                                                                                                       |                                                                                                             ttCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTg                                                                                                          >  1:566438/1‑139 (MQ=255)         aTCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTaa                                                                                                  >  2:1640662/1‑139 (MQ=255)                   atcaACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAAt                                                                                        >  1:841582/1‑139 (MQ=255)                        caaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggcg                                                                                   <  2:1537540/139‑1 (MQ=255)                        caaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCT‑ATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggcgg                                                                                  >  2:133456/1‑139 (MQ=255)                                gTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           <  1:104495/139‑1 (MQ=255)                                  aCCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                         <  1:110120/139‑1 (MQ=255)                                         cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                                    >  1:156017/1‑137 (MQ=255)                                         cTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGAt                                                                    <  2:156017/137‑1 (MQ=255)                                                 gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                          <  1:1467913/139‑1 (MQ=255)                                                 gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                          <  1:1588683/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  2:624422/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  2:1201130/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  1:229348/139‑1 (MQ=255)                                                    aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCT‑ATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGt                                                      <  1:133456/139‑1 (MQ=255)                                                      cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCGGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           >  1:1008921/1‑117 (MQ=255)                                                      cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           <  2:1008921/117‑1 (MQ=255)                                                      cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  1:176374/137‑1 (MQ=255)                                                      cccAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       >  2:176374/1‑137 (MQ=255)                                                         aaccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtatta                                                  <  2:1313545/139‑1 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                         >  1:609846/1‑114 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag                                                                         <  2:609846/114‑1 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  1:1119288/132‑1 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       >  2:1119288/1‑132 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatt                                                >  2:728122/1‑139 (MQ=255)                                                           ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatt                                                >  2:1310728/1‑139 (MQ=255)                                                              acGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATg                                             <  1:728122/139‑1 (MQ=255)                                                                gaagaaCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCt                                           <  2:97362/139‑1 (MQ=255)                                                                      cTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATccc                                     >  1:1533191/1‑139 (MQ=255)                                                                       tGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCt                                    <  1:1310728/139‑1 (MQ=255)                                                                         gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTAc                                  >  1:944800/1‑139 (MQ=255)                                                                              cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           >  2:710134/1‑93 (MQ=255)                                                                              cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           <  1:710134/93‑1 (MQ=255)                                                                              cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       >  1:134253/1‑113 (MQ=255)                                                                              cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  2:134253/113‑1 (MQ=255)                                                                              cgcgCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGaa                             >  1:868675/1‑139 (MQ=255)                                                                               gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAAc                            <  2:841582/139‑1 (MQ=255)                                                                                   aaGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  1:861493/108‑1 (MQ=255)                                                                                   aaGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       >  2:861493/1‑108 (MQ=255)                                                                                   aaGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGc                        >  2:623683/1‑139 (MQ=255)                                                                                    aGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg                       <  1:1640662/139‑1 (MQ=255)                                                                                    aGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg                       <  1:623683/139‑1 (MQ=255)                                                                                          gAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTc                 <  1:945739/139‑1 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggcgg                                                                                  >  1:346400/1‑60 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggcgg                                                                                  <  2:346400/60‑1 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgtg                                                            >  1:1625592/1‑82 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCgtg                                                            <  2:1625592/82‑1 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg                       <  1:428030/119‑1 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg                       >  2:428030/1‑119 (MQ=255)                                                                                                        ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGc   >  1:1311270/1‑139 (MQ=255)                                                                                                         cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc  <  2:1311270/139‑1 (MQ=255)                                                                                                                      gCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                          <  2:1336067/70‑1 (MQ=255)                                                                                                                      gCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc                                                          >  1:1336067/1‑70 (MQ=255)                                                                                                                       ccAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           <  2:1325604/52‑1 (MQ=255)                                                                                                                       ccAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           >  1:1325604/1‑52 (MQ=255)                                                                                                                       ccAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc  >  1:724322/1‑125 (MQ=255)                                                                                                                       ccAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc  <  2:724322/125‑1 (MQ=255)                                                                                                                           ctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           <  2:793617/48‑1 (MQ=255)                                                                                                                           ctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa                                                                           >  1:793617/1‑48 (MQ=255)                                                                                                                               gctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGta                                                     <  1:868075/66‑1 (MQ=255)                                                                                                                               gctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGta                                                     >  2:868075/1‑66 (MQ=255)                                                                                                                                  ggcggcGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       <  2:349140/61‑1 (MQ=255)                                                                                                                                  ggcggcGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg                                                       >  1:349140/1‑61 (MQ=255)                                                                                                                                       |                                                                                                             TTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCC  >  NC_000913/2414565‑2414807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑