Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,472,605 G→A R93W (CGG→TGG)  yfdN ← CPS‑53 (KpLE1) prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,472,6050GA100.0% 147.4 / NA 48R93W (CGG→TGG) yfdNCPS‑53 (KpLE1) prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/27);  total (21/27)

GCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTATCTCCTTCACAACGGTGAGCCA  >  NC_000913/2472473‑2472736
                                                                                                                                    |                                                                                                                                   
gcgcCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTc                                                                                                                               >  2:1180853/1‑139 (MQ=255)
  gcCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGt                                                                                                                             >  1:186291/1‑139 (MQ=255)
         aaTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                                                      <  2:1279979/139‑1 (MQ=255)
             gTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACa                                                                                                                  <  2:1623974/139‑1 (MQ=255)
                           gggAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGcac                                                                                                    <  1:687134/139‑1 (MQ=255)
                                  gCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                                             >  1:1144428/1‑139 (MQ=255)
                                  gCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                                             >  2:291859/1‑139 (MQ=255)
                                  gCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                                             >  1:1633543/1‑139 (MQ=255)
                                                   ccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                                            <  2:1356649/139‑1 (MQ=255)
                                                            ccGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCCGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                                            <  2:571238/130‑1 (MQ=255)
                                                            ccGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCCGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                                            >  1:571238/1‑130 (MQ=255)
                                                             cGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                                            >  2:38191/1‑129 (MQ=255)
                                                             cGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                                            <  1:38191/129‑1 (MQ=255)
                                                                tGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACa                                                                                                                  >  2:1250994/1‑88 (MQ=255)
                                                                tGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACa                                                                                                                  <  1:1250994/88‑1 (MQ=255)
                                                                    gcaCGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTgcg                                                           <  1:21621/139‑1 (MQ=255)
                                                                     caCGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTgcgc                                                          <  1:616215/139‑1 (MQ=255)
                                                                       cgacgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGctc                                                        <  2:20794/139‑1 (MQ=255)
                                                                          cgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGctctct                                                     >  1:1568431/1‑139 (MQ=255)
                                                                          cgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGctctct                                                     <  2:1633543/139‑1 (MQ=255)
                                                                              ataAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCtcagt                                                 <  1:291859/139‑1 (MQ=255)
                                                                                    cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGgcg                                           <  1:1180853/139‑1 (MQ=255)
                                                                                    cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGgcg                                           <  2:1133301/139‑1 (MQ=255)
                                                                                      cTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGcgcg                                         <  1:656827/139‑1 (MQ=255)
                                                                                       tGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                                                      >  2:1124760/1‑61 (MQ=255)
                                                                                       tGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCgg                                        >  2:1386577/1‑139 (MQ=255)
                                                                                       tGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCgg                                        >  2:59897/1‑139 (MQ=255)
                                                                                       tGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCgg                                        >  1:962818/1‑139 (MQ=255)
                                                                                       tGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATAACCCACGGGTCGTTAGTGcc                                                                                                                      <  1:1124760/61‑1 (MQ=255)
                                                                                          aCTCCAGTTCTTCCGCCTGGCGGGTCAGGCATAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGaa                                     <  2:377227/139‑1 (MQ=255)
                                                                                              cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGt                                 <  2:1568431/139‑1 (MQ=255)
                                                                                               aGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTc                                <  1:59897/139‑1 (MQ=255)
                                                                                                gTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCt                               <  1:402790/139‑1 (MQ=255)
                                                                                                    ttcCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCtgtgt                           >  1:1138400/1‑139 (MQ=255)
                                                                                                      ccGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTgcg                                                           >  2:1306678/1‑105 (MQ=255)
                                                                                                      ccGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTgcg                                                           <  1:1306678/105‑1 (MQ=255)
                                                                                                        gCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGgcg                                           >  2:863156/1‑119 (MQ=255)
                                                                                                        gCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGgcg                                           <  1:863156/119‑1 (MQ=255)
                                                                                                        gCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTAt                       <  1:585938/139‑1 (MQ=255)
                                                                                                        gCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTAt                       >  1:1032909/1‑139 (MQ=255)
                                                                                                              cGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTATCTCCtt                 >  2:1143208/1‑139 (MQ=255)
                                                                                                                   cAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGa                                      >  2:1156099/1‑113 (MQ=255)
                                                                                                                   cAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGa                                      <  1:1156099/113‑1 (MQ=255)
                                                                                                                             aTCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTATCTCCTTCACAACGGTGAGCca  <  1:1143208/139‑1 (MQ=255)
                                                                                                                                aCCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCGCGaa                                                                                   <  2:794194/55‑1 (MQ=255)
                                                                                                                                aCCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTATCTCCTTcac              >  1:842789/1‑124 (MQ=255)
                                                                                                                                aCCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTATCTCCTTcac              <  2:842789/124‑1 (MQ=255)
                                                                                                                                aCCCACGGGTCGTTAGTACCGACATAGAAATTGCGCACAGGTCTGGTTTCGCGaa                                                                                   >  1:794194/1‑55 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTGTGTATCTCCTTCACAACGGTGAGCCA  >  NC_000913/2472473‑2472736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑