| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,552,094 | G→A | I15I (ATC→ATT) | ypeA ← | GNAT family putative N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,552,094 | 0 | G | A | 98.4% | 193.6 / ‑4.3 | 63 | I15I (ATC→ATT) | ypeA | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (29/33); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTG‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTG > NC_000913/2551962‑2552221 | cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGAcc > 1:426429/1‑139 (MQ=255) aCCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCtt < 1:1211646/139‑1 (MQ=255) aCCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCtt > 1:643001/1‑139 (MQ=255) cACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACCCTCGATGTCCATTTCCGGAGCGTTCCACGGACGCAGCAAGTCTCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTcc < 2:186622/139‑1 (MQ=255) gTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑Tgg > 1:1098447/1‑139 (MQ=255) cACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCg < 2:31972/139‑1 (MQ=255) tttACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATa > 1:768130/1‑139 (MQ=255) ccTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATAcgcg < 2:426429/139‑1 (MQ=255) ccTCAACGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATAcgcg < 2:1498697/139‑1 (MQ=255) cTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt < 2:27821/139‑1 (MQ=255) tCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTa < 2:778187/139‑1 (MQ=255) cGACCAAAAACAAACTGACGTGATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCTACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAtctc < 2:315485/139‑1 (MQ=255) aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCCTTGGCGAAATACGCGTATCTcc < 2:104506/139‑1 (MQ=255) aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCa < 1:4510/139‑1 (MQ=255) aacTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGt < 2:1138221/139‑1 (MQ=255) aacTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGt < 2:888169/139‑1 (MQ=255) acTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGtt > 1:556038/1‑139 (MQ=255) aTGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATAcgcg < 2:368880/111‑1 (MQ=255) aTGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATAcgcg > 1:368880/1‑111 (MQ=255) ggTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCg < 2:1098447/139‑1 (MQ=255) aTCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTa > 2:109692/1‑139 (MQ=255) tACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg < 2:556038/139‑1 (MQ=255) aCGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAgg < 1:797229/139‑1 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt < 2:194809/99‑1 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt > 1:194809/1‑99 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAt > 2:1432458/1‑101 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAt < 1:1432458/101‑1 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAt > 1:159846/1‑101 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAt < 2:159846/101‑1 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGc > 2:1337137/1‑139 (MQ=255) gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt < 1:1601172/139‑1 (MQ=255) gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt > 2:1601172/1‑139 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGaaa < 1:1637219/86‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGaaa > 2:1637219/1‑86 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTa > 2:377890/1‑139 (MQ=255) aTGTCCATTTCCGGATCGATCCACGGACGCCGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCACATCGAAATCTTCC‑TGGCGAAACACGCGCATATCCATGGCATCCGTTACCTTTTTGCGGGTTAAAAGCCTGATTa > 2:797229/1‑139 (MQ=255) tGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt > 2:116162/1‑92 (MQ=255) tGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt < 1:116162/92‑1 (MQ=255) tGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTAt > 1:295041/1‑139 (MQ=255) tttCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGATA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt > 2:1120605/1‑86 (MQ=255) tttCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGATA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt < 1:1120605/86‑1 (MQ=255) cGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt > 2:971948/1‑82 (MQ=255) cGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGt < 1:971948/82‑1 (MQ=255) gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATAcgcg > 1:721820/1‑79 (MQ=255) gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATAcgcg < 2:721820/79‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑Tgg < 2:134760/63‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑Tgg > 1:134760/1‑63 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg < 2:170306/102‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg > 1:170306/1‑102 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt < 2:295041/139‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTGTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 2:1042091/1‑139 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCATAGCTCCATGGTATTCGTTACCTTTTTGATGGTAAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 2:861650/1‑139 (MQ=255) aCGCAGCAAGTCGCAACGCTCCCAAAGGGGGTA‑‑ATGACCTCTTCGAAATCTTCA‑TGGCGAAATACGCGTATCTCCATGGTa > 2:1435219/1‑81 (MQ=255) aCGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGGGTAATGACCTCTTCGAAATCTTCA‑TGGCGAAATACGCGTATCTCCATGGTa < 1:1435219/81‑1 (MQ=255) gcagcaAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCg < 2:988303/94‑1 (MQ=255) gcagcaAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTCCg > 1:988303/1‑94 (MQ=255) cagcaAGTCGCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATc > 2:550071/1‑139 (MQ=255) gCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAt < 1:150538/57‑1 (MQ=255) gCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTAt > 2:150538/1‑57 (MQ=255) gCAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGaaaa < 1:1337137/139‑1 (MQ=255) cAACGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAAt < 1:1042091/139‑1 (MQ=255) aaCGCTCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATc < 1:377890/139‑1 (MQ=255) tCCCAAA‑‑GGGTA‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTg > 1:354313/1‑139 (MQ=255) | CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAA‑‑GGGTG‑‑ATGACCTCTTCGAAATCTTCC‑TGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTG > NC_000913/2551962‑2552221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |