Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,608,367 | T→C | I484T (ATC→ACC) | hyfF → | hydrogenase 4, membrane subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,608,367 | 0 | T | C | 100.0% | 273.8 / NA | 75 | I484T (ATC→ACC) | hyfF | hydrogenase 4, membrane subunit |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (35/40); total (35/40) |
CTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCA > NC_000913/2608235‑2608501 | cTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGAt > 1:696820/1‑139 (MQ=255) cTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGAt > 1:185993/1‑139 (MQ=255) cgGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTgg > 1:1534835/1‑139 (MQ=255) gggTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTG‑‑‑ACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 2:741379/139‑1 (MQ=255) tgtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 1:1016559/139‑1 (MQ=255) tgtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 1:861302/139‑1 (MQ=255) tgtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 2:696820/139‑1 (MQ=255) tgtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGACGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 2:371382/139‑1 (MQ=255) gtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcgggc < 2:691733/139‑1 (MQ=255) ttAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcgggcg > 2:825848/1‑139 (MQ=255) tAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc < 2:1534835/139‑1 (MQ=255) aaTGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCt < 1:325211/139‑1 (MQ=255) aTGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCtt < 2:204443/139‑1 (MQ=255) tGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 2:1214471/139‑1 (MQ=255) tGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 1:825848/139‑1 (MQ=255) tGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCCGTCACCAGGATCCTGGCGGGCGCTTc < 1:745608/139‑1 (MQ=255) gCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCa < 1:1633143/139‑1 (MQ=255) cGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 2:1232556/130‑1 (MQ=255) cGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg > 1:1232556/1‑130 (MQ=255) gAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcg < 2:1595997/127‑1 (MQ=255) gAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcg > 1:1595997/1‑127 (MQ=255) cgccgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt < 1:1242355/139‑1 (MQ=255) cgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc > 2:528439/1‑127 (MQ=255) cgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 1:528439/127‑1 (MQ=255) ggTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc > 1:869353/1‑107 (MQ=255) ggTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc < 2:869353/107‑1 (MQ=255) tctcGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATctgcct > 1:1345777/1‑139 (MQ=255) ggCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt < 2:1598065/110‑1 (MQ=255) ggCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt > 1:1598065/1‑110 (MQ=255) gTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGt > 2:248134/1‑139 (MQ=255) gTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGt > 1:751134/1‑139 (MQ=255) gACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGc > 2:250334/1‑139 (MQ=255) caccTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCAcc < 2:751134/139‑1 (MQ=255) cTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTcc > 2:1439521/1‑98 (MQ=255) cTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTcc < 1:1439521/98‑1 (MQ=255) cGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCa > 2:212273/1‑139 (MQ=255) cGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCa > 1:286378/1‑139 (MQ=255) gTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAttttt < 1:212273/139‑1 (MQ=255) aTTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCctct < 2:24005/88‑1 (MQ=255) aTTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCctct > 1:24005/1‑88 (MQ=255) aTTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGAt > 1:133846/1‑102 (MQ=255) aTTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGAt < 2:133846/102‑1 (MQ=255) ttCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAg > 2:17562/1‑90 (MQ=255) ttCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAg < 1:17562/90‑1 (MQ=255) ctgctgGGCATGATGCTGGCGATGGGGACCCATATTCCACAGCCTGTCACCAGGATCCTGGCGGGCGCTTCCACTAAAGACCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCtt < 2:520218/139‑1 (MQ=255) gctgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCa > 2:1436211/1‑139 (MQ=255) gctgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCa < 2:214887/139‑1 (MQ=255) gctgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCa > 1:214887/1‑139 (MQ=255) ctgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACtata < 2:1336352/75‑1 (MQ=255) ctgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACtata > 1:1336352/1‑75 (MQ=255) tCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCTGGCAc < 2:519832/139‑1 (MQ=255) tGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCctct < 2:149362/69‑1 (MQ=255) tGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCctct > 1:149362/1‑69 (MQ=255) gCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc < 1:803314/50‑1 (MQ=255) gCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc > 2:803314/1‑50 (MQ=255) gggAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGgag > 2:1661290/1‑139 (MQ=255) gggAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGgag > 1:1127923/1‑139 (MQ=255) gAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAg < 1:436192/63‑1 (MQ=255) gAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAg > 2:436192/1‑63 (MQ=255) atTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTg < 1:1661290/139‑1 (MQ=255) atTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTg < 2:463415/139‑1 (MQ=255) ccTGTCGCCAGGATCCTGGCGGGCGCTTCCACTATAGt < 1:1074193/38‑1 (MQ=255) ccTGTCGCCAGGATCCTGGCGGGCGCTTCCACTATAGt > 2:1074193/1‑38 (MQ=255) cTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAAcaca < 2:65422/126‑1 (MQ=255) cTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAAcaca > 1:65422/1‑126 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAttttt > 2:59104/1‑88 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAttttt < 1:59104/88‑1 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCa < 2:1472706/105‑1 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCa > 1:1472706/1‑105 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGttt < 2:1243729/112‑1 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGttt > 1:1243729/1‑112 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTg < 2:654326/127‑1 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTg > 1:654326/1‑127 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:907976/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:907977/1‑139 (MQ=255) tcaccaGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTc > 2:330218/1‑94 (MQ=255) tcaccaGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTc < 1:330218/94‑1 (MQ=255) | CTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCA > NC_000913/2608235‑2608501 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |