Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,639,838	G→A	S246S (AGC→AGT)	hisS ←	histidyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,639,838	0	G	A	100.0%	183.6 / NA	56	S246S (AGC→AGT)	hisS	histidyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/26);  total (30/26)

AGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGT  >  NC_000913/2639704‑2639972                                                                                                                                       |                                                                                                                                       aGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCActct                                                                                                                                    >  2:1375903/1‑139 (MQ=255)  gACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACtctc                                                                                                                                   >  1:533324/1‑139 (MQ=255)    ccGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCca                                                                                                                                 >  1:1159258/1‑139 (MQ=255)      gTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCcagc                                                                                                                               <  1:162863/139‑1 (MQ=255)            aCGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTg                                                                                                                         >  1:1264391/1‑139 (MQ=255)               aCCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGcac                                                                                                                      >  1:1619782/1‑139 (MQ=255)                                    cTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACga                                                                                                 >  1:1031059/1‑139 (MQ=255)                                      gggAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACgaga                                                                                               >  2:623179/1‑139 (MQ=255)                                         aGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTc                                                                                            <  2:1334132/139‑1 (MQ=255)                                              agaCTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGt                                                                                       >  2:585525/1‑139 (MQ=255)                                               gaCTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTc                                                                                      <  2:1429998/139‑1 (MQ=255)                                                aCTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTcc                                                                                     <  2:1159258/139‑1 (MQ=255)                                                    ttAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGAt                                                                                 <  2:1264391/139‑1 (MQ=255)                                                     tAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATa                                                                                >  2:1456490/1‑139 (MQ=255)                                                      aGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAg                                                                               >  2:1645486/1‑139 (MQ=255)                                                       gTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGt                                                                              <  1:1216168/139‑1 (MQ=255)                                                                tCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATg                                                                     >  1:7127/1‑139 (MQ=255)                                                                 cGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGc                                                                    <  2:740191/139‑1 (MQ=255)                                                                   aaaaCGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCg                                                                  <  2:1031059/139‑1 (MQ=255)                                                                   aaaaCGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCg                                                                  <  2:7127/139‑1 (MQ=255)                                                                     aaCGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGa                                                                <  1:663270/139‑1 (MQ=255)                                                                                   agtaATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGTG‑TCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAg                                                               >  1:625309/1‑125 (MQ=255)                                                                                   agtaATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGTG‑TCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAg                                                               <  2:625309/125‑1 (MQ=255)                                                                                        tCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAg                                                               <  1:836300/121‑1 (MQ=255)                                                                                        tCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAg                                                               >  2:836300/1‑121 (MQ=255)                                                                                         ccAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTcc                                                                                           <  1:612525/92‑1 (MQ=255)                                                                                         ccAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTcc                                                                                           >  2:612525/1‑92 (MQ=255)                                                                                         ccAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTg                                            >  1:106504/1‑139 (MQ=255)                                                                                         ccAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTg                                            >  2:1024365/1‑139 (MQ=255)                                                                                         ccAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTg                                            <  1:1375903/139‑1 (MQ=255)                                                                                            gACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTg                                            >  1:1597336/1‑136 (MQ=255)                                                                                            gACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTg                                            <  2:1597336/136‑1 (MQ=255)                                                                                               cACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCt                                                                         >  2:1641054/1‑104 (MQ=255)                                                                                               cACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCt                                                                         <  1:1641054/104‑1 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCg                                                                  >  2:896899/1‑109 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCg                                                                  <  1:896899/109‑1 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAg                                                               >  1:1624653/1‑112 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAg                                                               <  2:1624653/112‑1 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGtt                                                        >  2:902269/1‑119 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGtt                                                        <  1:902269/119‑1 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCg                                    >  1:1278856/1‑139 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCg                                    >  1:807895/1‑139 (MQ=255)                                                                                                 cGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCg                                    >  1:1616789/1‑139 (MQ=255)                                                                                                  gCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCca                                                                                                                                 >  1:1576621/1‑45 (MQ=255)                                                                                                  gCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCACTCTCca                                                                                                                                 <  2:1576621/45‑1 (MQ=255)                                                                                                            cTGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGaa                         >  2:1616658/1‑139 (MQ=255)                                                                                                             tGGTTTACGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAAt                        >  2:1360879/1‑139 (MQ=255)                                                                                                                   aCGGTGTAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCa                  <  1:1645486/139‑1 (MQ=255)                                                                                                                         tAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCa            <  2:533324/139‑1 (MQ=255)                                                                                                                         tAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCa            >  1:1581996/1‑139 (MQ=255)                                                                                                                         tAAGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCa            <  2:1616789/139‑1 (MQ=255)                                                                                                                          aaGCGATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAg           <  1:148493/139‑1 (MQ=255)                                                                                                                              gATCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCggg       <  1:623179/139‑1 (MQ=255)                                                                                                                               aTCCCCGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGt      <  2:1458603/139‑1 (MQ=255)                                                                                                                                 ccccGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTa    >  1:935663/1‑139 (MQ=255)                                                                                                                                   ccGCACTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAgt  >  2:860423/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       AGACCGTCATAACGACCGCCTGCACACACGGTGCCCTGGGAGCCGAGACTGTTAGTCACCCACTCGAAAACGGTACGGTTGTAGTAATCCAGACCACGCACCAGACGCTGGTTTACGGTGTAAGCGATCCCCGCGCTCTCCAGCAGTTTGCACAGACCGGCAAAATGCTCACGAGATTCCTCGTCCAGATAGTCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGT  >  NC_000913/2639704‑2639972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑