Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 255,659 A→G C20R (TGT→CGT)  pepD ← aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913255,6590AG99.0% 348.4 / NA 97C20R (TGT→CGT) pepDaminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (48/48);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCTGCGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255524‑255793
                                                                                                                                       |                                                                                                                                      
tACCTGCGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGAt                                                                                                                                     >  1:1520615/1‑139 (MQ=255)
   cTGCGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGAtttt                                                                                                                                  >  2:1591037/1‑139 (MQ=255)
      cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  2:1582478/139‑1 (MQ=255)
      cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  2:429017/1‑139 (MQ=255)
            cAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAat                                                                                                                         >  1:262314/1‑139 (MQ=255)
                     gAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  1:1591037/139‑1 (MQ=255)
                          aGGATATTACCTTCCTGATCGCGTTCGACATGGAATCCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  1:999494/139‑1 (MQ=255)
                            gATATTACCTACCTGATCGCGTTCGACATGGAAATCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  2:1558823/117‑1 (MQ=255)
                            gATATTACCTACCTGATCGCGTTCGACATGGAAATCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  1:1558823/1‑117 (MQ=255)
                            gATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgt                                                                                                         <  1:233203/139‑1 (MQ=255)
                                ttACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:1520615/139‑1 (MQ=255)
                                ttACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:878354/139‑1 (MQ=255)
                                ttACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:1050552/139‑1 (MQ=255)
                                ttACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:1603613/139‑1 (MQ=255)
                                ttACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTAGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:245472/139‑1 (MQ=255)
                                 tacctaccTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  2:1636484/1‑112 (MQ=255)
                                 tacctaccTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  1:1636484/112‑1 (MQ=255)
                                 tacctaccTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgaga                                                                                                    >  1:165526/1‑139 (MQ=255)
                                      acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  1:22384/1‑107 (MQ=255)
                                      acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               <  2:22384/107‑1 (MQ=255)
                                      acctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAAtt                                                                                               >  2:1081162/1‑139 (MQ=255)
                                        ctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgtg                                                                                                        >  1:1081956/1‑128 (MQ=255)
                                        ctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCtgtg                                                                                                        <  2:1081956/128‑1 (MQ=255)
                                          gATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  1:1523073/1‑139 (MQ=255)
                                          gATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  2:1015169/1‑139 (MQ=255)
                                           aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCATCCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGAtt                                                                                                                                    <  2:1006864/97‑1 (MQ=255)
                                           aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  2:1215618/1‑137 (MQ=255)
                                           aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  1:1215618/137‑1 (MQ=255)
                                           aTCGCGTTCGACATAGAAACCTTTCTCTTTTGCCCATCCAACAATGTATTCAGCGAGTTGCTCTTCATGATATGACGGGTGAGGAATAGAACGGAtt                                                                                                                                    >  1:1006864/1‑97 (MQ=255)
                                             cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:1088562/126‑1 (MQ=255)
                                             cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  1:1088562/1‑126 (MQ=255)
                                             cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGAGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAg                                                                                        >  2:1062234/1‑139 (MQ=255)
                                               cgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAGCGGATTTTGGc                                                                                                                               <  1:1446547/98‑1 (MQ=255)
                                               cgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  2:1446547/1‑98 (MQ=255)
                                               cgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGtt                                                                                      <  2:1558871/139‑1 (MQ=255)
                                                     aCATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  1:144364/1‑118 (MQ=255)
                                                     aCATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  2:144364/118‑1 (MQ=255)
                                                     aCATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGaca                                                                                >  1:610444/1‑139 (MQ=255)
                                                       aTGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACg                                                                              <  2:165526/139‑1 (MQ=255)
                                                                 ttCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  <  1:500887/125‑1 (MQ=255)
                                                                 ttCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  >  2:500887/1‑125 (MQ=255)
                                                                  tctcTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCt                                                                   >  2:1578666/1‑139 (MQ=255)
                                                                    tctTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:608990/103‑1 (MQ=255)
                                                                    tctTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:608990/1‑103 (MQ=255)
                                                                         tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  1:181571/90‑1 (MQ=255)
                                                                         tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  2:181571/1‑90 (MQ=255)
                                                                         tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  1:1625513/108‑1 (MQ=255)
                                                                         tGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  2:1625513/1‑108 (MQ=255)
                                                                          gCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  1:431123/139‑1 (MQ=255)
                                                                                  aacaaTGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   <  1:1578666/139‑1 (MQ=255)
                                                                                     aaTGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAAc                                                >  1:77452/1‑139 (MQ=255)
                                                                                         tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  >  2:755900/1‑101 (MQ=255)
                                                                                         tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  <  1:755900/101‑1 (MQ=255)
                                                                                         tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTaa                                                                          <  1:1009330/109‑1 (MQ=255)
                                                                                         tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTaa                                                                          >  2:1009330/1‑109 (MQ=255)
                                                                                         tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            >  1:893700/1‑139 (MQ=255)
                                                                                          aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTcc                                                                    >  2:1602699/1‑114 (MQ=255)
                                                                                          aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTcc                                                                    <  1:1602699/114‑1 (MQ=255)
                                                                                          aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTAt                                           >  1:1138516/1‑139 (MQ=255)
                                                                                          aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTAt                                           >  1:548294/1‑139 (MQ=255)
                                                                                            tCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTg                                         <  2:1523073/139‑1 (MQ=255)
                                                                                              aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       >  2:916767/1‑139 (MQ=255)
                                                                                              aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCCCTTGTCGATCACCCGCAAAACAGTATTGCAg                                      >  2:1020390/1‑139 (MQ=255)
                                                                                               gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCAcc                                                        <  1:365354/121‑1 (MQ=255)
                                                                                               gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCAcc                                                        >  2:365354/1‑121 (MQ=255)
                                                                                                    ttGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:1285407/1‑71 (MQ=255)
                                                                                                    ttGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:1285407/71‑1 (MQ=255)
                                                                                                     tGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGt                                                                <  1:345925/107‑1 (MQ=255)
                                                                                                     tGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGt                                                                >  2:345925/1‑107 (MQ=255)
                                                                                                          ttCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGtt                           <  2:610876/139‑1 (MQ=255)
                                                                                                             aTGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGc                        <  2:893700/139‑1 (MQ=255)
                                                                                                              tGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  >  2:154826/1‑80 (MQ=255)
                                                                                                              tGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGa                                                                                  <  1:154826/80‑1 (MQ=255)
                                                                                                                 tAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGaa                    >  1:610795/1‑139 (MQ=255)
                                                                                                                    gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           <  2:967641/49‑1 (MQ=255)
                                                                                                                    gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCt                                                                                                           >  1:967641/1‑49 (MQ=255)
                                                                                                                    gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  2:1255739/65‑1 (MQ=255)
                                                                                                                    gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  1:1255739/1‑65 (MQ=255)
                                                                                                                    gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          <  1:1028314/66‑1 (MQ=255)
                                                                                                                    gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          >  2:1028314/1‑66 (MQ=255)
                                                                                                                         gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  1:429017/139‑1 (MQ=255)
                                                                                                                         gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            >  1:1250034/1‑139 (MQ=255)
                                                                                                                         gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            >  1:194762/1‑139 (MQ=255)
                                                                                                                         gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            <  1:250/139‑1 (MQ=255)
                                                                                                                          tGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTc           >  2:752574/1‑139 (MQ=255)
                                                                                                                            aGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCaa         <  2:610795/139‑1 (MQ=255)
                                                                                                                             ggAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAac        <  2:1250034/139‑1 (MQ=255)
                                                                                                                              gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  1:439662/139‑1 (MQ=255)
                                                                                                                              gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       <  1:752574/139‑1 (MQ=255)
                                                                                                                                  aGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAg   >  1:953828/1‑139 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           <  2:1376019/82‑1 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATc                                                           >  1:1376019/1‑82 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaaaa                                                 <  1:1360932/92‑1 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaaaa                                                 >  2:1360932/1‑92 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   <  2:382523/90‑1 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   >  1:382523/1‑90 (MQ=255)
                                                                                                                                   gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGa  >  1:637822/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TACCTGCGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255524‑255793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: