Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,699,413	G→A	A219T (GCC→ACC)	yfhH →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,699,413	0	G	A	100.0%	286.3 / NA	87	A219T (GCC→ACC)	yfhH	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/42);  total (45/42)

CAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGC  >  NC_000913/2699281‑2699542                                                                                                                                     |                                                                                                                                  cAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGc                                                                                                                             >  1:540293/1‑139 (MQ=255)      gtcgtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAg                                                                                                                        >  2:17528/1‑139 (MQ=255)       tcgtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGc                                                                                                                       >  2:177892/1‑139 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGt                                                                                                                     >  2:924385/1‑139 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGt                                                                                                                     >  1:976775/1‑139 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGt                                                                                                                     >  1:1601708/1‑139 (MQ=255)         gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGC‑‑TACTCCGAATACCCTGCAACAGCGTGc                                                                                                                   >  2:935736/1‑139 (MQ=255)             ccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCtt                                                                                                                 <  2:976775/139‑1 (MQ=255)                 aCGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGC‑‑TACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                           <  1:935736/139‑1 (MQ=255)                   gATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                           <  1:41362/139‑1 (MQ=255)                   gATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                           <  1:147844/139‑1 (MQ=255)                   gATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                           <  2:1419077/139‑1 (MQ=255)                     tCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         <  2:858503/139‑1 (MQ=255)                             taGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATAc                                                                                                 <  1:1289697/139‑1 (MQ=255)                             taGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATAc                                                                                                 <  2:540293/139‑1 (MQ=255)                              aGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTCGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGTCTGTATAcc                                                                                                >  2:142368/1‑139 (MQ=255)                               gCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         <  1:1250897/129‑1 (MQ=255)                               gCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         >  2:1250897/1‑129 (MQ=255)                                    ttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGcc                                                                                          <  2:337156/139‑1 (MQ=255)                                    ttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGcc                                                                                          >  2:1204993/1‑139 (MQ=255)                                          cacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCg                                                                                         <  1:870158/134‑1 (MQ=255)                                          cacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCg                                                                                         >  2:870158/1‑134 (MQ=255)                                              ggTGTACGACGCGAGTTAATCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACTCTGCAACAGCGTGCTTCTCATTGc                                                                                                         <  2:481393/114‑1 (MQ=255)                                              ggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         >  1:481393/1‑114 (MQ=255)                                              ggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAg                                                                                >  2:775030/1‑139 (MQ=255)                                               gtgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGc                                                                               >  2:1378813/1‑139 (MQ=255)                                               gtgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGc                                                                               >  1:1249668/1‑139 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGCAAAGTGCTGGCGATTACCGGCATTCCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTACCGAAGAACAAGCg                                                                              >  1:1015463/1‑139 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         <  2:1232929/112‑1 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         >  1:1232929/1‑112 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCg                                                                              >  2:732115/1‑139 (MQ=255)                                                tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCg                                                                              >  2:943044/1‑139 (MQ=255)                                                     gacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         >  1:1282093/1‑107 (MQ=255)                                                     gacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         <  2:1282093/107‑1 (MQ=255)                                                       cgcgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACa                                                                       >  2:1351049/1‑139 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCATCAGCGTGCTTCTCATTG‑CTGTATACCATTGCCGAAGAACAAGCGACAAACAGTg                                                                    >  2:1088479/1‑139 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                           <  1:26504/101‑1 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAtt                                                                                                           >  2:26504/1‑101 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAt                                                                                                            >  2:227166/1‑100 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCAt                                                                                                            <  1:227166/100‑1 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCa                                                                                               <  2:1543642/113‑1 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCa                                                                                               >  1:1543642/1‑113 (MQ=255)                                                         cgAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                     <  1:1378813/139‑1 (MQ=255)                                                          gAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTg                                                                    >  2:700091/1‑139 (MQ=255)                                                            gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGc                                                                                                                             >  2:724630/1‑80 (MQ=255)                                                            gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGc                                                                                                                             <  1:724630/80‑1 (MQ=255)                                                              tAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTc                                                                <  1:732115/139‑1 (MQ=255)                                                                  ccTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtc                                                            <  2:513599/139‑1 (MQ=255)                                                                   cTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtct                                                           <  1:943044/139‑1 (MQ=255)                                                                     ggcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCt                                                                  >  1:648280/1‑130 (MQ=255)                                                                     ggcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCt                                                                  <  2:648280/130‑1 (MQ=255)                                                                     ggcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATctct                                                         >  2:42398/1‑139 (MQ=255)                                                                      gcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCg                                                                                                                      <  1:1488280/77‑1 (MQ=255)                                                                      gcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCg                                                                                                                      >  2:1488280/1‑77 (MQ=255)                                                                      gcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTg                                                        >  2:748366/1‑139 (MQ=255)                                                                      gcggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTg                                                        >  2:215878/1‑139 (MQ=255)                                                                          cAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                         <  2:1363264/118‑1 (MQ=255)                                                                          cAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                         >  1:1363264/1‑118 (MQ=255)                                                                          cAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTG‑CTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGt                                                   <  1:1088479/139‑1 (MQ=255)                                                                            gatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGc                                                                                                                   >  2:1365212/1‑74 (MQ=255)                                                                            gatgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGc                                                                                                                   <  1:1365212/74‑1 (MQ=255)                                                                                    gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                         <  1:732988/108‑1 (MQ=255)                                                                                    gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                         >  2:732988/1‑108 (MQ=255)                                                                                            tGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                       >  1:244806/1‑118 (MQ=255)                                                                                            tGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                       <  2:244806/118‑1 (MQ=255)                                                                                              ggCGGAAAAGTGCTGGCGTTTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCAGCTTGTCACGCTCAGGGAATGTTaa                                >  2:1142930/1‑139 (MQ=255)                                                                                              ggCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTaa                                <  2:1249668/139‑1 (MQ=255)                                                                                                 ggAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACgg                             <  1:700091/139‑1 (MQ=255)                                                                                                     aaGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACg                                               >  1:587194/1‑117 (MQ=255)                                                                                                     aaGTGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACg                                               <  2:587194/117‑1 (MQ=255)                                                                                                        tGCTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCt                      >  1:1251926/1‑139 (MQ=255)                                                                                                          cTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         >  2:479941/1‑54 (MQ=255)                                                                                                          cTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGc                                                                                                         <  1:479941/54‑1 (MQ=255)                                                                                                          cTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAt                                                             >  2:1291913/1‑98 (MQ=255)                                                                                                          cTGGCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAt                                                             <  1:1291913/98‑1 (MQ=255)                                                                                                             gCGATTACCGGCTTTGCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGtt                                  >  2:1108139/1‑122 (MQ=255)                                                                                                             gCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGTCAAACTGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGtt                                  <  1:1108139/122‑1 (MQ=255)                                                                                                             gCGATTACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCa                 <  2:1334531/139‑1 (MQ=255)                                                                                                                 ttACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgc             >  2:1282713/1‑139 (MQ=255)                                                                                                                  tACCGGCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTgcg            <  1:1351049/139‑1 (MQ=255)                                                                                                                      ggCTTTACTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGa        <  2:636677/139‑1 (MQ=255)                                                                                                                            aCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGAtt      >  1:970935/1‑135 (MQ=255)                                                                                                                            aCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGAtt      <  2:970935/135‑1 (MQ=255)                                                                                                                            aCTCCGAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc  <  1:1282713/139‑1 (MQ=255)                                                                                                                                 gAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                       >  2:1544906/1‑81 (MQ=255)                                                                                                                                 gAATACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                       <  1:1544906/81‑1 (MQ=255)                                                                                                                                  aaTACCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGa                                                                             <  1:580913/58‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  CAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGC  >  NC_000913/2699281‑2699542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑