Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,742,962	G→A	D194N (GAT→AAT)	yfiN →	putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,742,962	0	G	A	100.0%	156.0 / NA	49	D194N (GAT→AAT)	yfiN	putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/29);  total (20/29)

CAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTT  >  NC_000913/2742829‑2743090                                                                                                                                      |                                                                                                                                 cAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTc                                                                                                                             <  2:234699/139‑1 (MQ=255)              cATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGc                                                                                                                >  1:128364/1‑139 (MQ=255)                           tttCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCaa                                                                                                   >  1:1471970/1‑139 (MQ=255)                            ttCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAAt                                                                                                  >  2:742247/1‑139 (MQ=255)                                       tACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                       <  2:1024691/139‑1 (MQ=255)                                            aCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGttt                                                                                  <  1:864157/139‑1 (MQ=255)                                             ccGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTc                                                                                 >  2:1342057/1‑139 (MQ=255)                                                     aTTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAAc                                                                         <  2:285742/139‑1 (MQ=255)                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACatgatg                                                                                                                   <  1:1240370/91‑1 (MQ=255)                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACatgatg                                                                                                                   >  2:1240370/1‑91 (MQ=255)                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAAcc                                                                                                       <  2:719799/103‑1 (MQ=255)                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAAcc                                                                                                       >  1:719799/1‑103 (MQ=255)                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                   >  2:135391/1‑139 (MQ=255)                                                            tgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                  <  1:742247/139‑1 (MQ=255)                                                                   aGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAAt                                                                                                  <  2:180810/100‑1 (MQ=255)                                                                   aGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAAt                                                                                                  >  1:180810/1‑100 (MQ=255)                                                                          gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCt                                                    <  1:1118705/139‑1 (MQ=255)                                                                             aTTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCg                                                 <  2:128364/139‑1 (MQ=255)                                                                                aCCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCg                                                                                                                            <  2:1340516/61‑1 (MQ=255)                                                                                aCCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCg                                                                                                                            >  1:1340516/1‑61 (MQ=255)                                                                                aCCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctc                                              <  1:581798/139‑1 (MQ=255)                                                                                  ccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                            <  2:1471970/139‑1 (MQ=255)                                                                                  ccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                            <  2:339492/139‑1 (MQ=255)                                                                                  ccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                            <  2:592644/139‑1 (MQ=255)                                                                                      aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACt                                        >  2:512182/1‑139 (MQ=255)                                                                                          gCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCa                                     <  2:283157/138‑1 (MQ=255)                                                                                          gCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCa                                     >  1:283157/1‑138 (MQ=255)                                                                                            cATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACa                                  <  2:737316/139‑1 (MQ=255)                                                                                              tttGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGt                                <  2:990440/139‑1 (MQ=255)                                                                                                 gCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                             <  2:167200/139‑1 (MQ=255)                                                                                                 gCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                             <  2:119623/139‑1 (MQ=255)                                                                                                  cacaATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTAGATGATGAGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctgc                            >  2:958249/1‑139 (MQ=255)                                                                                                      aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                         >  2:983244/1‑74 (MQ=255)                                                                                                      aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                         <  1:983244/74‑1 (MQ=255)                                                                                                        ggCCTGGTAGAAGCGCTGAAAAATATCATCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATg                      <  1:917755/139‑1 (MQ=255)                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAATAGTCTGCTGGATGaa                    >  2:462143/1‑139 (MQ=255)                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa                    >  2:862131/1‑139 (MQ=255)                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa                    >  1:407935/1‑139 (MQ=255)                                                                                                            tGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                         >  1:701966/1‑68 (MQ=255)                                                                                                            tGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                         <  2:701966/68‑1 (MQ=255)                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCa                                                                                                    <  2:1481234/50‑1 (MQ=255)                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCa                                                                                                    >  1:1481234/1‑50 (MQ=255)                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctc                                              >  1:1633021/1‑104 (MQ=255)                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctc                                              <  2:1633021/104‑1 (MQ=255)                                                                                                                      gcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTgg        <  2:901096/139‑1 (MQ=255)                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa      <  1:1171220/139‑1 (MQ=255)                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa      <  1:1342057/139‑1 (MQ=255)                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa      <  1:135391/139‑1 (MQ=255)                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa      >  2:1171220/1‑139 (MQ=255)                                                                                                                            aaaTATCACCAAGGTCGTACATGATGTGCGTTCCAACCGCAATTTTGCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTGTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCtt  <  2:539337/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 CAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTT  >  NC_000913/2742829‑2743090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑