Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,814,218 C→T *172* (TAG→TAA)  luxS ← S‑ribosylhomocysteine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,2180CT100.0% 162.1 / NA 51*172* (TAG→TAA) luxSS‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/27);  total (24/27)

TCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGT  >  NC_000913/2814084‑2814342
                                                                                                                                      |                                                                                                                            
tCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGa                                                                                                                          <  1:260200/139‑1 (MQ=255)
     ctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGc                                                                                                                     <  2:502087/139‑1 (MQ=255)
     ctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGc                                                                                                                     >  2:1518715/1‑139 (MQ=255)
      tgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCa                                                                                                                    <  1:376680/139‑1 (MQ=255)
             ggTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTg                                                                                                             >  1:1207529/1‑139 (MQ=255)
               tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCa                                                                                                           >  2:856307/1‑139 (MQ=255)
                        cTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGtt                                                                                                                 <  2:413007/124‑1 (MQ=255)
                        cTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGtt                                                                                                                 >  1:413007/1‑124 (MQ=255)
                             ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTGAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATCGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                             <  1:1106879/139‑1 (MQ=255)
                             ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                             <  2:1207529/139‑1 (MQ=255)
                                                      gAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGa                                                                    <  2:988713/139‑1 (MQ=255)
                                                       aGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt                                                                   >  1:282113/1‑139 (MQ=255)
                                                       aGGCGGTGCGCACTAAGTACAACTAAGCCAATTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt                                                                   <  1:1170863/139‑1 (MQ=255)
                                                         gCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt                                                                             >  2:861507/1‑127 (MQ=255)
                                                         gCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt                                                                             <  1:861507/127‑1 (MQ=255)
                                                                 cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCa                                                         <  2:128874/139‑1 (MQ=255)
                                                                             cTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATg                                             >  1:1486948/1‑139 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGtt                                                                                                                 <  1:167894/68‑1 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGtt                                                                                                                 >  2:167894/1‑68 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCa                                                         <  1:1195536/124‑1 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCa                                                         >  2:1195536/1‑124 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                          >  1:1558552/1‑139 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                          >  2:1414604/1‑139 (MQ=255)
                                                                                aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                          >  1:1328671/1‑139 (MQ=255)
                                                                                  ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                                          >  2:335541/1‑121 (MQ=255)
                                                                                  ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                                          <  1:335541/121‑1 (MQ=255)
                                                                                  ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcg                                        >  2:1479392/1‑139 (MQ=255)
                                                                                      ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCa                                    <  1:856307/139‑1 (MQ=255)
                                                                                       tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACt                                                                                                        <  2:1659828/70‑1 (MQ=255)
                                                                                       tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACt                                                                                                        >  1:1659828/1‑70 (MQ=255)
                                                                                       tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg                                                                           <  2:248094/99‑1 (MQ=255)
                                                                                       tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTg                                                                           >  1:248094/1‑99 (MQ=255)
                                                                                          tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAatat                                >  2:1547210/1‑139 (MQ=255)
                                                                                            tGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATcc                              <  1:1518715/139‑1 (MQ=255)
                                                                                                   gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCa                                                                                                                    >  1:770712/1‑46 (MQ=255)
                                                                                                   gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCa                                                                                                                    <  2:770712/46‑1 (MQ=255)
                                                                                                   gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCtt                       >  2:1338230/1‑139 (MQ=255)
                                                                                                   gCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCtt                       >  1:1291120/1‑139 (MQ=255)
                                                                                                              ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg            <  1:1338230/139‑1 (MQ=255)
                                                                                                              ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg            <  2:1350830/139‑1 (MQ=255)
                                                                                                              ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg            <  1:1414604/139‑1 (MQ=255)
                                                                                                                   tgtgAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCTCGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCACCGAGTGCAt       >  2:907069/1‑139 (MQ=255)
                                                                                                                   tgtgAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCAt       <  1:1479392/139‑1 (MQ=255)
                                                                                                                       aaGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGtt                                                     >  2:1223813/1‑89 (MQ=255)
                                                                                                                       aaGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGtt                                                     <  1:1223813/89‑1 (MQ=255)
                                                                                                                        aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt  <  2:282113/139‑1 (MQ=255)
                                                                                                                        aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt  <  2:1291120/139‑1 (MQ=255)
                                                                                                                            aGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                                                                                        >  1:1368308/1‑49 (MQ=255)
                                                                                                                            aGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                                                                                        <  2:1368308/49‑1 (MQ=255)
                                                                                                                                 aCTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                                          <  1:921388/74‑1 (MQ=255)
                                                                                                                                 aCTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                                          >  2:921388/1‑74 (MQ=255)
                                                                                                                                      |                                                                                                                            
TCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGT  >  NC_000913/2814084‑2814342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑