Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,024	C→T	pseudogene (94/174 nt)	insI1 →	IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,024	0	C	T	100.0%	101.3 / NA	34	pseudogene (94/174 nt)	insI1	IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCG  >  NC_000913/279893‑280149                                                                                                                                    |                                                                                                                              acCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAaacaa                                                                                                                        <  1:34506/139‑1 (MQ=255)  ccATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAaacaac                                                                                                                       >  1:47680/1‑139 (MQ=255)     tcatACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGa                                                                                                                    <  2:1444047/139‑1 (MQ=255)          cacTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCgag                                                                                                               <  1:1352794/139‑1 (MQ=255)             tAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGaaa                                                                                                            >  1:1279365/1‑139 (MQ=255)               aaTCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGa                                                                                                          >  2:1625417/1‑139 (MQ=255)                    gTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTg                                                                                                     <  1:20969/139‑1 (MQ=255)                     tAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATCTACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGa                                                                                                    >  2:1184725/1‑139 (MQ=255)                     tAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAaacaa                                                                                                                        >  2:611856/1‑119 (MQ=255)                     tAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAaacaa                                                                                                                        <  1:611856/119‑1 (MQ=255)                              agcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaaaa                                                                                           <  2:1279365/139‑1 (MQ=37)                                caTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAaca                                                                                         <  1:1184725/139‑1 (MQ=25)                                  tCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACAcc                                                                                       >  2:1560088/1‑139 (MQ=18)                                    aCCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGa                                                                                     <  2:47680/139‑1 (MQ=17)                                                                                       cTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCt                                  <  1:37338/139‑1 (MQ=18)                                                                                           cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                              >  1:575350/1‑139 (MQ=25)                                                                                           cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                              <  2:1181270/139‑1 (MQ=21)                                                                                           cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                              >  2:1586429/1‑139 (MQ=21)                                                                                                     tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                    >  1:1491096/1‑139 (MQ=255)                                                                                                     tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAAACTACAGCAGCTTTTTTTAATATGTc                    >  2:1243403/1‑139 (MQ=255)                                                                                                          aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               >  1:818342/1‑139 (MQ=255)                                                                                                          aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               >  1:956819/1‑139 (MQ=255)                                                                                                          aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               >  1:1298205/1‑139 (MQ=255)                                                                                                          aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               >  1:12722/1‑139 (MQ=255)                                                                                                            gAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTtcg             >  1:439903/1‑139 (MQ=255)                                                                                                            gAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTtcg             >  2:1398215/1‑139 (MQ=255)                                                                                                                     cTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAAcc    <  2:1491096/139‑1 (MQ=255)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               <  2:965174/126‑1 (MQ=255)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               >  1:965174/1‑126 (MQ=255)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCg  >  1:1248784/1‑139 (MQ=255)                                                                                                                        gTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                    <  2:1479944/120‑1 (MQ=255)                                                                                                                        gTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                    >  1:1479944/1‑120 (MQ=255)                                                                                                                            ctgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               >  2:284787/1‑121 (MQ=255)                                                                                                                            ctgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt               <  1:284787/121‑1 (MQ=255)                                                                                                                                    |                                                                                                                              ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCG  >  NC_000913/279893‑280149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑