Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,902,543 G→A G216G (GGG→GGA ygcE → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,902,5430GA98.8% 259.2 / NA 81G216G (GGG→GGAygcEputative kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (41/39);  minor base C (0/1);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTG  >  NC_000913/2902411‑2902675
                                                                                                                                    |                                                                                                                                    
tatGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGa                                                                                                                                <  2:536409/139‑1 (MQ=255)
tatGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGa                                                                                                                                >  1:494972/1‑139 (MQ=255)
     tGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg                                                                                                                                  <  1:756728/132‑1 (MQ=255)
     tGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg                                                                                                                                  >  2:756728/1‑132 (MQ=255)
                cAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaaa                                                                                                                >  2:1353235/1‑139 (MQ=255)
                      ccTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcggcgg                                                                                                          >  2:414915/1‑139 (MQ=255)
                          gCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAAt                                                                                                      >  1:50629/1‑139 (MQ=255)
                                tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                    >  2:523579/1‑103 (MQ=255)
                                tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGc                                                                                                                                    <  1:523579/103‑1 (MQ=255)
                                tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGa                                                                                                                                <  2:1367116/107‑1 (MQ=255)
                                tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGa                                                                                                                                >  1:1367116/1‑107 (MQ=255)
                                tCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTctct                                                                                                >  1:599710/1‑139 (MQ=255)
                                   tCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTgg                                                                                             <  2:487184/139‑1 (MQ=255)
                                    cGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcggc                                                                                                            >  2:783788/1‑123 (MQ=255)
                                    cGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAAcggc                                                                                                            <  1:783788/123‑1 (MQ=255)
                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                 <  2:889308/128‑1 (MQ=255)
                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                 >  1:889308/1‑128 (MQ=255)
                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTAc                                                                                      >  1:832949/1‑139 (MQ=255)
                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTAc                                                                                      >  1:638896/1‑139 (MQ=255)
                                          tatCACACCACAAGCCGCACTGGCGACACATTCCCCGGCAGGACTGCCGGTTGTTTGTACCACCGGTGATAAACCGGTAGAAGCTCTGGGCGCCGGATTACAGGATGATGAAACGGCGGTAAGTTCTCTTGGCAGTTAc                                                                                      <  2:1203173/139‑1 (MQ=255)
                                           atCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACa                                                                                     >  2:728677/1‑139 (MQ=255)
                                           atCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACa                                                                                     >  1:504289/1‑139 (MQ=255)
                                            tCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACAt                                                                                    <  2:599710/139‑1 (MQ=255)
                                                 ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAt                                                                               >  1:1523836/1‑139 (MQ=255)
                                                   acaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtg                                                                             >  1:1007087/1‑139 (MQ=255)
                                                    caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTa                                                                                                                             >  1:1010500/1‑90 (MQ=255)
                                                    caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTa                                                                                                                             <  2:1010500/90‑1 (MQ=255)
                                                     aaGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg                                                                                                                                  <  1:1397535/84‑1 (MQ=255)
                                                     aaGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCg                                                                                                                                  >  2:1397535/1‑84 (MQ=255)
                                                     aaGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgat                                                                           >  1:1286139/1‑139 (MQ=255)
                                                     aaGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgat                                                                           >  1:732358/1‑139 (MQ=255)
                                                       gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaaa                                                                                                                >  2:1512803/1‑100 (MQ=255)
                                                       gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGaaa                                                                                                                <  1:1512803/100‑1 (MQ=255)
                                                       gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatga                                                                         >  2:1354403/1‑139 (MQ=255)
                                                       gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatga                                                                         >  2:1576950/1‑139 (MQ=255)
                                                         cGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgatg                                                                       <  2:832949/139‑1 (MQ=255)
                                                         cGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgatg                                                                       <  1:1354403/139‑1 (MQ=255)
                                                                   acacATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGa                                                                                                                                <  1:945406/72‑1 (MQ=255)
                                                                   acacATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGa                                                                                                                                >  2:945406/1‑72 (MQ=255)
                                                                      caTTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCAc                                                          <  2:1437971/139‑1 (MQ=255)
                                                                          tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGTAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc                                                      >  1:131156/1‑139 (MQ=255)
                                                                          tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCAc                                                                                          <  1:914710/103‑1 (MQ=255)
                                                                          tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCAc                                                                                          >  2:914710/1‑103 (MQ=255)
                                                                             cGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGaa                                                   <  2:1503799/139‑1 (MQ=255)
                                                                                 aGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGAt                                               >  1:401657/1‑139 (MQ=255)
                                                                                  ggACTGCTGGTTGTTTGTACCCCCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATTAAACGGCGGTAATTTCTCTTGGCACTTCCATCGCATTGATGATGAACTGCAAAGCACTGCCGAATGacc                                              <  2:963337/139‑3 (MQ=255)
                                                                                  ggACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATc                                              <  1:872555/139‑1 (MQ=255)
                                                                                   gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc                                             >  2:401767/1‑139 (MQ=255)
                                                                                   gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc                                             >  1:749016/1‑139 (MQ=255)
                                                                                      tGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAt                                                                               >  1:14198/1‑102 (MQ=255)
                                                                                      tGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAt                                                                               <  2:14198/102‑1 (MQ=255)
                                                                                      tGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGt                                          <  1:428610/139‑1 (MQ=255)
                                                                                          ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAt                                                                               <  1:501282/98‑1 (MQ=255)
                                                                                          ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAt                                                                               >  2:501282/1‑98 (MQ=255)
                                                                                            ttgtttgtACCACCAGTGATAAACCGGTAGTAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTa                                    <  2:131156/139‑1 (MQ=255)
                                                                                            ttgtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTa                                    <  1:1576950/139‑1 (MQ=255)
                                                                                               tttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTg                                 <  1:414915/139‑1 (MQ=255)
                                                                                                   tACCACCAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCg                             <  2:1523836/139‑1 (MQ=255)
                                                                                                    accaccAGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGa                            <  2:504289/139‑1 (MQ=255)
                                                                                                      caccaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGAtt                          <  1:401767/139‑1 (MQ=255)
                                                                                                        ccaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTAt                        >  1:63216/1‑139 (MQ=255)
                                                                                                        ccaGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTCGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTAt                        <  2:1287778/139‑1 (MQ=255)
                                                                                                         caGTGATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATg                       >  1:489004/1‑139 (MQ=255)
                                                                                                             gATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                   <  1:998565/139‑1 (MQ=255)
                                                                                                             gATAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtctt                   <  2:1286139/139‑1 (MQ=255)
                                                                                                              aTAAACCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGtcttc                  >  2:802999/1‑139 (MQ=255)
                                                                                                                aaaCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTa                <  2:494972/139‑1 (MQ=255)
                                                                                                                aaaCCGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTa                <  2:638896/139‑1 (MQ=255)
                                                                                                                   ccGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                 >  1:685919/1‑55 (MQ=255)
                                                                                                                   ccGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                 <  2:685919/55‑1 (MQ=255)
                                                                                                                   ccGGTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTc             >  2:1534419/1‑139 (MQ=255)
                                                                                                                      gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCa                                                                                           <  2:1270165/58‑1 (MQ=255)
                                                                                                                      gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCa                                                                                           >  1:1270165/1‑58 (MQ=255)
                                                                                                                      gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATc                                              <  2:487626/103‑1 (MQ=255)
                                                                                                                      gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATc                                              >  1:487626/1‑103 (MQ=255)
                                                                                                                      gTAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGc          >  1:692723/1‑139 (MQ=255)
                                                                                                                       tAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                 <  1:506312/51‑1 (MQ=255)
                                                                                                                       tAGAAGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTtctc                                                                                                 >  2:506312/1‑51 (MQ=255)
                                                                                                                           aGCTCTGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACa     <  1:728677/139‑1 (MQ=255)
                                                                                                                              tctGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg  <  2:1007087/139‑1 (MQ=255)
                                                                                                                              tctGGGAGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTg  >  2:1216143/1‑139 (MQ=255)
                                                                                                                                 gggagCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCg                                                     <  1:181913/85‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
TATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACTGGCCGATTATGTCTTCTATTCCGCAAACATTG  >  NC_000913/2902411‑2902675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: