Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,962,441 C→T *108* (TAG→TAA ppdC ← putative prepilin peptidase‑dependent protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,962,4410CT100.0% 191.1 / NA 59*108* (TAG→TAAppdCputative prepilin peptidase‑dependent protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/25);  total (34/25)

GTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCG  >  NC_000913/2962310‑2962574
                                                                                                                                   |                                                                                                                                     
gTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGAc                                                                                                                                >  2:205835/1‑139 (MQ=255)
gTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGAc                                                                                                                                >  1:1543434/1‑139 (MQ=255)
  aCTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGa                                                                                                                              >  1:1001764/1‑139 (MQ=255)
      tGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCg                                                                                                                          <  2:755794/139‑1 (MQ=255)
        cACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCggg                                                                                                                        >  1:688156/1‑139 (MQ=255)
                     tctGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCggg                                                                                                           <  1:425531/139‑1 (MQ=255)
                     tctGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCggg                                                                                                           >  2:843390/1‑139 (MQ=255)
                     tctGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCggg                                                                                                           >  1:261837/1‑139 (MQ=255)
                          ttCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAtc                                                                                                      >  1:199625/1‑139 (MQ=255)
                             gAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCg                                                                                                   <  1:1019867/139‑1 (MQ=255)
                               aaGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGcc                                                                                                 <  2:890493/139‑1 (MQ=255)
                                          aGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  2:759674/139‑1 (MQ=255)
                                                      tcgACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAAGCGGGTCATCTCGCCTTCGCTTCCCCGGGGTGAAACTa                                                                          >  2:1244713/1‑139 (MQ=255)
                                                          cAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGt                                                                      <  1:205835/139‑1 (MQ=255)
                                                          cAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGt                                                                      <  2:261837/139‑1 (MQ=255)
                                                                    cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCa                                                                                                                 <  2:1143682/86‑1 (MQ=255)
                                                                    cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCa                                                                                                                 >  1:1143682/1‑86 (MQ=255)
                                                                    cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            >  2:175448/1‑139 (MQ=255)
                                                                    cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            >  1:917940/1‑139 (MQ=255)
                                                                       cAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCGGGTGAAACTAGCGTAACGCTGATGCTGa                                                        <  2:1001764/139‑1 (MQ=255)
                                                                             ttCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACAt                                                   <  1:175448/139‑1 (MQ=255)
                                                                               ccAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATcc                                                 >  1:1475470/1‑139 (MQ=255)
                                                                                      gTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGc                                          <  1:1266591/139‑1 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCggg                                                                                                           >  2:371199/1‑73 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCggg                                                                                                           <  1:371199/73‑1 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGa                                                        >  2:1639089/1‑124 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGa                                                        <  1:1639089/124‑1 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  1:731878/1‑139 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  1:254131/1‑139 (MQ=255)
                                                                                               aTTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGc                                 >  1:1359696/1‑139 (MQ=255)
                                                                                                ttGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTtct                                                                                             <  2:65015/78‑1 (MQ=255)
                                                                                                ttGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTtct                                                                                             >  1:65015/1‑78 (MQ=255)
                                                                                                ttGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCa                                >  2:127313/1‑139 (MQ=255)
                                                                                                        gTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTg                        >  2:1633714/1‑139 (MQ=255)
                                                                                                           gACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGAcc                     >  1:1062477/1‑139 (MQ=255)
                                                                                                           gACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGAcc                     <  2:1475470/139‑1 (MQ=255)
                                                                                                             cccTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTg                   <  2:917940/139‑1 (MQ=255)
                                                                                                              ccTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGc                  >  2:65349/1‑139 (MQ=255)
                                                                                                                 tAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  2:770811/68‑1 (MQ=255)
                                                                                                                 tAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      >  1:770811/1‑68 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGAcc                     <  2:1417367/127‑1 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGAcc                     >  1:1417367/1‑127 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCg                                                                                                   <  2:128865/48‑1 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCg                                                                                                   >  1:128865/1‑48 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCggg                                                                                  >  1:1080084/1‑65 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCggg                                                                                  <  2:1080084/65‑1 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAAc                                                                            >  2:97263/1‑71 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAAc                                                                            <  1:97263/71‑1 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCt                                                          <  2:3263/89‑1 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCt                                                          >  1:3263/1‑89 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  2:1459262/1‑139 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  1:360188/1‑139 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAgg       >  2:37947/1‑139 (MQ=255)
                                                                                                                         cGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAgg       >  2:855675/1‑139 (MQ=255)
                                                                                                                          ggCTCCTGATTACTGACGATTCGGGCAATTCAGGCGGGTCATCTCGc                                                                                                  <  2:946993/47‑1 (MQ=255)
                                                                                                                          ggCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGc                                                                                                  >  1:946993/1‑47 (MQ=255)
                                                                                                                            cTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCAtt                              <  2:1602715/113‑1 (MQ=255)
                                                                                                                            cTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCAtt                              >  1:1602715/1‑113 (MQ=255)
                                                                                                                              ccTGATTACTGGCGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGtcaac  <  1:127313/139‑5 (MQ=255)
                                                                                                                                   |                                                                                                                                     
GTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCG  >  NC_000913/2962310‑2962574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: