Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,051,321 G→A R333C (CGT→TGT)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,051,3210GA100.0% 130.8 / NA 41R333C (CGT→TGT) ubiI2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/22);  total (19/22)

AGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCG  >  NC_000913/3051185‑3051451
                                                                                                                                        |                                                                                                                                  
aGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGAcaca                                                                                                                                  <  1:523257/139‑1 (MQ=255)
    tgtCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATa                                                                                                                              <  1:805721/139‑1 (MQ=255)
     gtCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATaa                                                                                                                             <  2:1545023/139‑1 (MQ=255)
         gCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGt                                                                                                                         <  2:779512/139‑1 (MQ=255)
                                cagcagTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGa                                                                                                  <  1:1250141/139‑1 (MQ=255)
                                 agcagTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGAc                                                                                                 >  1:620183/1‑139 (MQ=255)
                                   cagTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGa                                                                                               >  1:506438/1‑139 (MQ=255)
                                    agTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGAt                                                                                              <  1:1086926/139‑1 (MQ=255)
                                                          cGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt                                                                        <  2:1354133/139‑1 (MQ=255)
                                                          cGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt                                                                        <  1:1510165/139‑1 (MQ=255)
                                                          cGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt                                                                        <  2:620183/139‑1 (MQ=255)
                                                            gAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCa                                                                      >  1:1251429/1‑139 (MQ=255)
                                                            gAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCa                                                                      >  1:256113/1‑139 (MQ=255)
                                                                 cAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGctct                                                                 <  1:1036635/139‑1 (MQ=255)
                                                                    aTCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTgca                                                              >  1:1554954/1‑139 (MQ=255)
                                                                      cgcgGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTgcagc                                                            >  2:14463/1‑139 (MQ=255)
                                                                                  gCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGcc                                                <  1:456540/139‑1 (MQ=255)
                                                                                     tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt                                                                        >  1:321507/1‑112 (MQ=255)
                                                                                     tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt                                                                        <  2:321507/112‑1 (MQ=255)
                                                                                     tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCgag                                             >  1:1622547/1‑139 (MQ=255)
                                                                                        cagccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGct                                                                   <  2:1378501/114‑1 (MQ=255)
                                                                                        cagccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGct                                                                   >  1:1378501/1‑114 (MQ=255)
                                                                                         agccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGAttt                                         >  1:934201/1‑139 (MQ=255)
                                                                                             agcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccc                                     >  2:1660839/1‑139 (MQ=255)
                                                                                               cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt                                                                                                          <  2:1449731/68‑1 (MQ=255)
                                                                                               cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt                                                                                                          >  1:1008269/1‑68 (MQ=255)
                                                                                               cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt                                                                                                          <  2:1008269/68‑1 (MQ=255)
                                                                                               cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt                                                                                                          >  1:1449731/1‑68 (MQ=255)
                                                                                               cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                   >  2:577747/1‑139 (MQ=255)
                                                                                                          cACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAg                        <  1:352323/139‑1 (MQ=255)
                                                                                                            cTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCg                      <  2:1251429/139‑1 (MQ=255)
                                                                                                               tgCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGt                   >  1:1604257/1‑139 (MQ=255)
                                                                                                               tgCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGt                   >  2:1480292/1‑139 (MQ=255)
                                                                                                                    gCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGc                                                                                            <  2:972801/61‑1 (MQ=255)
                                                                                                                    gCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGc                                                                                            >  1:972801/1‑61 (MQ=255)
                                                                                                                              tCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcgg    <  1:14463/139‑1 (MQ=255)
                                                                                                                                aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGAt                                           >  1:804527/1‑98 (MQ=255)
                                                                                                                                aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGAt                                           <  2:804527/98‑1 (MQ=255)
                                                                                                                                aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggcg  <  2:1309825/139‑1 (MQ=255)
                                                                                                                                aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggcg  <  1:1660839/139‑1 (MQ=255)
                                                                                                                                  aGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGtt                                                                               >  2:1101265/1‑60 (MQ=255)
                                                                                                                                  aGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGtt                                                                               <  1:1101265/60‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
AGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCG  >  NC_000913/3051185‑3051451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑