Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,085,461	A→G	S151P (TCC→CCC)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,085,461	0	A	G	100.0%	203.4 / NA	57	S151P (TCC→CCC)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

CCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTG  >  NC_000913/3085326‑3085591                                                                                                                                        |                                                                                                                                   ccAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                 >  1:401678/1‑139 (MQ=255)     aTAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCt                                                                                                                             >  1:1198499/1‑139 (MQ=255)          ccTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAg                                                                                                                        >  1:771555/1‑139 (MQ=255)            ttGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAc                                                                                                                      >  1:1163179/1‑139 (MQ=255)               tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCa                                                                                                                   >  1:277466/1‑139 (MQ=255)               tgCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCa                                                                                                                   >  2:464036/1‑139 (MQ=255)                   ccATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCgg                                                                                                               <  2:881641/139‑1 (MQ=255)                     aTCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGtt                                                                                                             >  2:568449/1‑139 (MQ=255)                        ttctCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                          <  1:757425/139‑1 (MQ=255)                        ttctCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                          <  1:551520/139‑1 (MQ=255)                           tcGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCg                                                                                                       >  2:531332/1‑139 (MQ=255)                            cGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCga                                                                                                      <  1:1153012/139‑1 (MQ=255)                             gCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaa                                                                                                     <  1:568449/139‑1 (MQ=255)                              ccAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                    <  2:771555/139‑1 (MQ=255)                              ccAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                    <  2:1598666/139‑1 (MQ=255)                                  ttAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaatgaat                                                                                                >  2:1641808/1‑139 (MQ=255)                                    aaTGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCa                                                                                              <  2:1271543/139‑1 (MQ=255)                                           ggCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTc                                                                                       >  2:707396/1‑139 (MQ=255)                                                         cgGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGt                                                                         <  2:1163179/139‑1 (MQ=255)                                                          ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                        <  1:707396/139‑1 (MQ=255)                                                                 ataACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTa                                                                 >  1:267869/1‑139 (MQ=255)                                                                   aaCCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAAc                                                               <  1:1605468/139‑1 (MQ=255)                                                                    aCCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCCGCGGTTCGCCCGAATGAATCAGGTACTCAATCACGCCGCGGTGCTGGTTAACt                                                              >  2:106729/1‑139 (MQ=255)                                                                      cGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                        >  1:574810/1‑127 (MQ=255)                                                                      cGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTg                                                                        <  2:574810/127‑1 (MQ=255)                                                                        ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                    >  1:307839/1‑97 (MQ=255)                                                                        ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                    <  2:307839/97‑1 (MQ=255)                                                                        ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                          >  1:103371/1‑139 (MQ=255)                                                                        ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGa                                                          >  1:247039/1‑139 (MQ=255)                                                                          gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                    <  1:1262054/79‑1 (MQ=255)                                                                          gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGAccc                                                                                                                    >  2:1262054/1‑79 (MQ=255)                                                                                  tGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGt                                                                                                              <  1:150556/77‑1 (MQ=255)                                                                                  tGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGt                                                                                                              >  2:150556/1‑77 (MQ=255)                                                                                  tGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                          >  2:872111/1‑81 (MQ=255)                                                                                  tGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGc                                                                                                          <  1:872111/81‑1 (MQ=255)                                                                                       cTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGa                                           <  2:103371/139‑1 (MQ=255)                                                                                        tACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGaa                                          >  2:1110051/1‑139 (MQ=255)                                                                                          cGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGt                                        >  1:1082039/1‑139 (MQ=255)                                                                                             gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCgg                                                                                                               >  2:1434479/1‑65 (MQ=255)                                                                                             gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCgg                                                                                                               <  1:1434479/65‑1 (MQ=255)                                                                                              tCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATc                                    <  1:77045/139‑1 (MQ=255)                                                                                                  gCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                 >  2:690382/1‑42 (MQ=255)                                                                                                  gCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGAcc                                                                                                                                 <  1:690382/42‑1 (MQ=255)                                                                                                     agaaTGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGATTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                             <  2:401678/136‑1 (MQ=255)                                                                                                     aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                             <  1:531332/139‑1 (MQ=255)                                                                                                      gCATGTGCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtat                            <  2:267869/139‑1 (MQ=255)                                                                                                           tgCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCg                       <  2:277466/139‑1 (MQ=255)                                                                                                            gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                             >  2:690058/1‑132 (MQ=255)                                                                                                            gCCAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTta                             <  1:690058/132‑1 (MQ=255)                                                                                                             ccAGTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTa                     <  1:31664/139‑1 (MQ=255)                                                                                                                gTACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGa                  <  1:633407/139‑1 (MQ=255)                                                                                                                 tACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATc                                                                                               >  2:1225515/1‑61 (MQ=255)                                                                                                                 tACTGCCATCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGAGGTTCGCCCGAATGAATc                                                                                               <  1:1225515/61‑1 (MQ=255)                                                                                                                         tCAACTCGGCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGc         >  2:607662/1‑139 (MQ=255)                                                                                                                                ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                    <  1:1244870/41‑1 (MQ=255)                                                                                                                                ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCgaat                                                                                                    >  2:1244870/1‑41 (MQ=255)                                                                                                                                ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTg  >  2:756156/1‑139 (MQ=255)                                                                                                                                ggCTTTGGGACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTg  >  1:1022801/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                   CCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTG  >  NC_000913/3085326‑3085591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑