Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,149	T→C	L173L (TTG→CTG)	ebgA →	evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,149	0	T	C	100.0%	274.1 / NA	76	L173L (TTG→CTG)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/37);  total (39/37)

ATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCAC  >  NC_000913/3223020‑3223276                                                                                                                                  |                                                                                                                                aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGTAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTgc                                                                                                                        >  2:396770/1‑139 (MQ=255)      aTTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTg                                                                                                                   <  1:1344725/139‑1 (MQ=255)           aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCa                                                                                                              >  1:1632234/1‑139 (MQ=255)               cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGT‑GGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTAACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         >  2:436416/1‑139 (MQ=255)                gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         <  2:1651648/139‑1 (MQ=255)                gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         <  2:449293/139‑1 (MQ=255)                gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         >  1:1651648/1‑139 (MQ=255)                  cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                       <  2:445562/139‑1 (MQ=255)                  cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                       <  1:1617657/139‑1 (MQ=255)                      aCCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGAct                                                                                                   <  1:1247421/139‑1 (MQ=255)                                  gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                       >  1:1655791/1‑139 (MQ=255)                                       tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         >  1:708370/1‑116 (MQ=255)                                       tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         <  2:708370/116‑1 (MQ=255)                                       tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAc                                                                                  <  1:628660/139‑1 (MQ=255)                                       tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAc                                                                                  >  2:1315257/1‑139 (MQ=255)                                        gTTAACGGTCAGTATGTGGGTTTCAGCAAGGGAAGTCGCCTGACCGCAGAGTTTGAAATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                 <  2:1655791/139‑1 (MQ=255)                                             cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTcta                                                                                                >  1:747781/1‑119 (MQ=255)                                             cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTcta                                                                                                <  2:747781/119‑1 (MQ=255)                                             cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGat                                                                            >  1:214117/1‑139 (MQ=255)                                               gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGatat                                                                          >  2:82763/1‑139 (MQ=255)                                                 cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAtgt                                                                        >  1:1206812/1‑139 (MQ=255)                                                  aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                       >  1:666158/1‑139 (MQ=255)                                                   gTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtgg                                                                      >  2:631670/1‑139 (MQ=255)                                                       gtgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTggtggt                                                                  <  1:1021951/139‑1 (MQ=255)                                                         gggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                       >  2:221709/1‑116 (MQ=255)                                                         gggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                       <  1:221709/116‑1 (MQ=255)                                                          ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                       >  2:785697/1‑99 (MQ=255)                                                          ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                       <  1:785697/99‑1 (MQ=255)                                                          ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                               >  1:1549604/1‑139 (MQ=255)                                                            tttCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCg                                                             >  2:541755/1‑139 (MQ=255)                                                              tcggCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgtg                                                                                                                           >  1:280398/4‑75 (MQ=255)                                                              tcggCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATAAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgtg                                                                                                                           <  2:280398/72‑1 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgt                                                                                                                            >  2:818523/1‑74 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgt                                                                                                                            <  1:818523/74‑1 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         <  2:230574/93‑1 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                         >  1:230574/1‑93 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGa                                                                                                     >  2:299027/1‑97 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGa                                                                                                     <  1:299027/97‑1 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                           >  2:225246/1‑139 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                           >  2:1001692/1‑139 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                           >  2:754012/1‑139 (MQ=255)                                                              tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                           >  2:427517/1‑139 (MQ=255)                                                                       gCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  <  1:783327/139‑1 (MQ=255)                                                                       gCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  <  2:666158/139‑1 (MQ=255)                                                                         aGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                <  1:427517/139‑1 (MQ=255)                                                                         aGTCGCCTGACCGCAGAGTTTAACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                <  1:436416/139‑1 (MQ=255)                                                                            cGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCTGCGATg                                             >  2:1557892/1‑139 (MQ=255)                                                                                      cAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  >  1:1388620/1‑124 (MQ=255)                                                                                      cAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  <  2:1388620/124‑1 (MQ=255)                                                                                      cAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGt                                   >  2:512225/1‑139 (MQ=255)                                                                                           tttGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGaa                              <  1:754012/139‑1 (MQ=255)                                                                                             tGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGa                                               >  2:374607/1‑120 (MQ=255)                                                                                             tGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGa                                               <  1:374607/120‑1 (MQ=255)                                                                                              gACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAac                           >  2:544840/1‑139 (MQ=255)                                                                                                   cAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTa                      >  2:789416/1‑139 (MQ=255)                                                                                                    aGCGCGATGGTTGCAGCCGGCGACAACCGGTGGTGGGGGCGCTTGATGCGGTGGGCGGACTCTGGCTACGTGGAAGACCAGGATATGTGGTGCGCAGAAGGGAATTTCCGCGATTGTTATCTGGTCGGAAAACGCCTaa                     <  2:753576/139‑1 (MQ=255)                                                                                                    aGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTaa                     <  2:1632234/139‑1 (MQ=255)                                                                                                     gcgcGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                       >  2:1117063/1‑72 (MQ=255)                                                                                                     gcgcGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                       <  1:1117063/72‑1 (MQ=255)                                                                                                     gcgcGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  >  2:394838/1‑109 (MQ=255)                                                                                                     gcgcGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  >  2:1615023/1‑109 (MQ=255)                                                                                                     gcgcGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  <  1:1615023/109‑1 (MQ=255)                                                                                                     gcgcGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  <  1:394838/109‑1 (MQ=255)                                                                                                       gcgATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGc                  <  2:214117/139‑1 (MQ=255)                                                                                                       gcgATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGc                  <  2:750404/139‑1 (MQ=255)                                                                                                         gATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCat                >  1:624443/1‑139 (MQ=255)                                                                                                          aTGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAgg                                                                              <  2:1209410/76‑1 (MQ=255)                                                                                                          aTGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAgg                                                                              >  1:1209410/1‑76 (MQ=255)                                                                                                            ggTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt             <  1:82763/139‑1 (MQ=255)                                                                                                              ttAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGa                                                                                                     >  1:800131/1‑49 (MQ=255)                                                                                                              ttAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGa                                                                                                     <  2:800131/49‑1 (MQ=255)                                                                                                              ttAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTaa           <  1:541755/139‑1 (MQ=255)                                                                                                              ttAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTaa           <  2:1549604/139‑1 (MQ=255)                                                                                                                     cGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTc    <  1:1557892/139‑1 (MQ=255)                                                                                                                       gCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCAc  >  1:1295363/1‑139 (MQ=255)                                                                                                                        cGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  >  2:1278235/1‑90 (MQ=255)                                                                                                                        cGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                  <  1:1278235/90‑1 (MQ=255)                                                                                                                                  |                                                                                                                                ATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCAC  >  NC_000913/3223020‑3223276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑