Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,478,592 C→T *67* (TAG→TAA yheV ← DUF2387 family putative metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,478,5920CT100.0% 278.5 / NA 85*67* (TAG→TAAyheVDUF2387 family putative metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/42);  total (43/42)

ATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACA  >  NC_000913/3478459‑3478722
                                                                                                                                     |                                                                                                                                  
aTAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAGGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTACCTTAAAAAAATTCGGGCAAAATCGTTAGTc                                                                                                                               >  2:382455/1‑139 (MQ=255)
     ccAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGAt                                                                                                                          >  1:1458490/1‑139 (MQ=255)
         gCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGCATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaa                                                                                                                      >  1:1118078/1‑139 (MQ=255)
          cTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     <  2:1118078/139‑1 (MQ=255)
          cTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     >  2:1641992/1‑139 (MQ=255)
                  caGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGAt                                                                                                             >  2:401313/1‑139 (MQ=255)
                   aGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                            <  2:538703/139‑1 (MQ=255)
                   aGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                            <  1:401313/139‑1 (MQ=255)
                   aGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                            <  1:1578457/139‑1 (MQ=255)
                    ggTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCa                                                                                                           <  2:1057635/139‑1 (MQ=255)
                       cTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     <  2:409072/126‑1 (MQ=255)
                       cTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGaaaa                                                                                                                     >  1:409072/1‑126 (MQ=255)
                         tttGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGc                                                                                                      <  2:777394/139‑1 (MQ=255)
                          ttGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCt                                                                                                     <  1:1270702/139‑1 (MQ=255)
                           tGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTc                                                                                                    >  2:747188/1‑139 (MQ=255)
                                        atatCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACg                                                                                       >  2:1521821/1‑139 (MQ=255)
                                          atCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTg                                                                                     >  1:1465294/1‑139 (MQ=255)
                                          atCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTg                                                                                     >  2:1295861/1‑139 (MQ=255)
                                            ctcCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGaa                                                                                         >  2:507215/1‑133 (MQ=255)
                                            ctcCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGaa                                                                                         <  1:507215/133‑1 (MQ=255)
                                             tcCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATc                                                                                  <  2:683050/139‑1 (MQ=255)
                                             tcCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATc                                                                                  >  1:1658350/1‑139 (MQ=255)
                                                  aGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCggg                                                                             <  1:1521821/139‑1 (MQ=255)
                                                    cATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGct                                                                           >  1:1081668/1‑139 (MQ=255)
                                                       gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttct                                                                        >  2:951909/1‑139 (MQ=255)
                                                       gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttct                                                                        >  1:869568/1‑139 (MQ=255)
                                                        ggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttctt                                                                       <  2:1465294/139‑1 (MQ=255)
                                                              aTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCATTGTCTg                                                                 >  2:1087628/1‑139 (MQ=255)
                                                                  tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtc                                                             >  1:1660349/1‑139 (MQ=255)
                                                                   ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                            <  2:1081668/139‑1 (MQ=255)
                                                                    gcgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtctc                                                           >  2:1595899/1‑139 (MQ=255)
                                                                    gcgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCACATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtctc                                                           >  2:658228/1‑139 (MQ=255)
                                                                     cgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCg                                                          <  1:951909/139‑1 (MQ=255)
                                                                       cAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCg                                                                                           <  2:324398/104‑1 (MQ=255)
                                                                       cAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCg                                                                                           >  1:324398/1‑104 (MQ=255)
                                                                       cAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCa                                                        >  2:624464/1‑139 (MQ=255)
                                                                        aGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCAt                                                       <  1:1641992/139‑1 (MQ=255)
                                                                          aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                     <  1:1380838/139‑1 (MQ=255)
                                                                              tAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              <  1:1627936/110‑1 (MQ=255)
                                                                              tAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              >  2:1627936/1‑110 (MQ=255)
                                                                               aGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCTAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                <  2:1134160/139‑1 (MQ=255)
                                                                                    aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGt                                                                                                                                >  1:304464/1‑54 (MQ=255)
                                                                                    aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGt                                                                                                                                <  2:304464/54‑1 (MQ=255)
                                                                                    aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCa                                           >  1:1197296/1‑139 (MQ=255)
                                                                                      ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                >  2:979886/1‑132 (MQ=255)
                                                                                      ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                <  1:979886/132‑1 (MQ=255)
                                                                                      ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACt                                         >  1:226239/1‑139 (MQ=255)
                                                                                      ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACt                                         >  2:1252753/1‑139 (MQ=255)
                                                                                        cTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTa                                       <  2:1660349/139‑1 (MQ=255)
                                                                                           cAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCt                                                                                                     <  1:1493772/74‑1 (MQ=255)
                                                                                           cAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCt                                                                                                     >  2:1493772/1‑74 (MQ=255)
                                                                                           cAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAaca                                    <  1:624464/139‑1 (MQ=255)
                                                                                             gCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAAcaca                                  <  2:869568/139‑1 (MQ=255)
                                                                                              ccGTGTACTCACTAGCTTAAAAAAACTCGGCGCATGTCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              <  2:1434850/94‑1 (MQ=255)
                                                                                              ccGTGTACTCACTAGCTTAAAAAAACTCGGCGCATGTCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              >  1:1434850/1‑94 (MQ=255)
                                                                                              ccGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACAt                                 <  1:1595899/139‑1 (MQ=255)
                                                                                                gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTc                               >  2:1566221/1‑139 (MQ=255)
                                                                                                 tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              <  1:29181/123‑1 (MQ=255)
                                                                                                 tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              >  2:29181/1‑123 (MQ=255)
                                                                                                 tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTca                              >  1:1620608/1‑139 (MQ=255)
                                                                                                  gtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaac                            <  2:524170/139‑1 (MQ=255)
                                                                                                        aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              >  1:284109/1‑116 (MQ=255)
                                                                                                        aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                              <  2:284109/116‑1 (MQ=255)
                                                                                                        aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatat                       >  2:1481081/1‑139 (MQ=255)
                                                                                                        aCTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatat                       <  1:1566221/139‑1 (MQ=255)
                                                                                                         cTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatc                      >  1:1574524/1‑139 (MQ=255)
                                                                                                          tAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatca                     <  2:1574524/139‑1 (MQ=255)
                                                                                                           aGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                     <  1:1215924/106‑1 (MQ=255)
                                                                                                           aGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                     >  2:1215924/1‑106 (MQ=255)
                                                                                                           aGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatcaa                    <  1:747188/139‑1 (MQ=255)
                                                                                                            gCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaa                             >  2:481627/1‑129 (MQ=255)
                                                                                                            gCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTcaa                             <  1:481627/129‑1 (MQ=255)
                                                                                                            gCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatcaat                   >  2:1028388/1‑139 (MQ=255)
                                                                                                             cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              >  2:860111/1‑79 (MQ=255)
                                                                                                             cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCgg                                                                              <  1:860111/79‑1 (MQ=255)
                                                                                                             cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                >  1:1113356/1‑109 (MQ=255)
                                                                                                             cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGt                                                <  2:1113356/109‑1 (MQ=255)
                                                                                                                aaaaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGt                                                                    >  1:1660072/1‑86 (MQ=255)
                                                                                                                aaaaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGt                                                                    <  2:1660072/86‑1 (MQ=255)
                                                                                                                    aaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCAt                                                                                                  >  1:1527864/1‑52 (MQ=255)
                                                                                                                    aaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCAt                                                                                                  <  2:1527864/52‑1 (MQ=255)
                                                                                                                       cTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTcgc        >  1:325796/1‑139 (MQ=255)
                                                                                                                         cGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCgcgc      <  2:1620608/139‑1 (MQ=255)
                                                                                                                            cgcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTcgc        >  1:1383834/1‑134 (MQ=255)
                                                                                                                            cgcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTcgc        <  2:1383834/134‑1 (MQ=255)
                                                                                                                             gcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCcaca  <  2:226239/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
ATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACA  >  NC_000913/3478459‑3478722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: