Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,547,986	C→T	*695* (TAG→TAA)	malQ ←	4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,547,986	0	C	T	100.0%	206.2 / NA	63	*695* (TAG→TAA)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/32);  total (31/32)

TCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGC  >  NC_000913/3547851‑3548119                                                                                                                                        |                                                                                                                                      tCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTAc                                                                                                                                    >  1:886508/1‑139 (MQ=255) tCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTAc                                                                                                                                    >  2:562612/1‑139 (MQ=255)              atGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                       <  2:1338850/139‑1 (MQ=255)              atGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                       <  2:371338/139‑1 (MQ=255)                ggTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCa                                                                                                                     <  1:56212/139‑1 (MQ=255)                  tGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGc                                                                                                                   >  1:1482788/1‑139 (MQ=255)                       tGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc                                                                                                              >  2:1052594/1‑139 (MQ=255)                       tGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc                                                                                                              >  1:176881/1‑139 (MQ=255)                            acacTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                         <  2:1482788/139‑1 (MQ=255)                                tgactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGt                                                                                                     >  1:666151/1‑139 (MQ=255)                                         gATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCt                                                                                            <  2:1640888/139‑1 (MQ=255)                                          aTGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCa                                                                                                                     >  1:316477/1‑113 (MQ=255)                                          aTGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCa                                                                                                                     <  2:316477/113‑1 (MQ=255)                                                cgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgcg                                                                                                             >  2:1429836/1‑115 (MQ=255)                                                cgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgcg                                                                                                             <  1:1429836/115‑1 (MQ=255)                                                    aCCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGc                                                                                       <  1:1526328/133‑1 (MQ=255)                                                    aCCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGc                                                                                       >  2:1526328/1‑133 (MQ=255)                                                         cTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTc                                                                                          >  2:1033463/1‑125 (MQ=255)                                                         cTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATAGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTc                                                                                          <  1:1033463/125‑1 (MQ=255)                                                                ggCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcatca                                                                     <  1:1052594/139‑1 (MQ=255)                                                                       gATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCaa                                                              >  2:1332803/1‑139 (MQ=255)                                                                            gggATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                         <  1:1519931/91‑1 (MQ=255)                                                                            gggATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                         >  2:1519931/1‑91 (MQ=255)                                                                                            ccGGATATGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCTGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                         >  2:1084095/1‑139 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCaaa                                                                                                >  1:605713/1‑84 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCaaa                                                                                                <  2:605713/84‑1 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGc                                                                                       >  2:1128702/1‑93 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGc                                                                                       <  1:1128702/93‑1 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                         >  1:1173247/1‑139 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                         >  1:485291/1‑139 (MQ=255)                                                                                            ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                         >  1:249537/1‑139 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                         >  2:1548393/1‑90 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                         <  1:1548393/90‑1 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAAc                                                            <  1:1146655/119‑1 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAAc                                                            >  2:1146655/1‑119 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATTGACTCAAGCGTTGCGGa                                        >  2:500821/1‑139 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCaa                                                  >  1:667104/1‑129 (MQ=255)                                                                                             cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCaa                                                  <  2:667104/129‑1 (MQ=255)                                                                                               gATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAAc                                                                                    >  2:936025/1‑93 (MQ=255)                                                                                               gATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAAc                                                                                    <  1:936025/93‑1 (MQ=255)                                                                                               gATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGaaa                                      >  2:1569806/1‑139 (MQ=255)                                                                                                    gACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTg                                 <  1:1569806/139‑1 (MQ=255)                                                                                                     aCGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGc                                <  1:189099/139‑1 (MQ=255)                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  2:1173247/139‑1 (MQ=255)                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  2:886508/139‑1 (MQ=255)                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  1:1400037/139‑1 (MQ=255)                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  2:238230/139‑1 (MQ=255)                                                                                                           cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  2:236939/139‑1 (MQ=255)                                                                                                                    catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                           <  2:562981/129‑1 (MQ=255)                                                                                                                    catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                           >  1:562981/1‑129 (MQ=255)                                                                                                                        cGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  2:238102/126‑1 (MQ=255)                                                                                                                        cGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          >  1:238102/1‑126 (MQ=255)                                                                                                                          gCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                           <  2:251781/123‑1 (MQ=255)                                                                                                                          gCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                           >  1:251781/1‑123 (MQ=255)                                                                                                                            aTCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGt                                              >  2:25946/1‑102 (MQ=255)                                                                                                                            aTCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGt                                              <  1:25946/102‑1 (MQ=255)                                                                                                                            aTCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTaa         >  2:172670/1‑139 (MQ=255)                                                                                                                                aCCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAATTCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTgg     <  2:432983/139‑1 (MQ=255)                                                                                                                                   gCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGc  <  2:176881/139‑1 (MQ=255)                                                                                                                                    cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAAc                                                                                    >  2:255370/1‑56 (MQ=255)                                                                                                                                    cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAAc                                                                                    <  1:255370/56‑1 (MQ=255)                                                                                                                                    cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTaa         <  1:1050088/131‑1 (MQ=255)                                                                                                                                    cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTaa         >  2:1050088/1‑131 (MQ=255)                                                                                                                                        |                                                                                                                                      TCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGC  >  NC_000913/3547851‑3548119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑