Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,587,370 C→T T143I (ACT→ATT) 
*248* (TAG→TAA) 		 yhhA →
ugpQ ←		 DUF2756 family protein
glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,587,3700CT100.0% 232.2 / NA 71T143I (ACT→ATT) 
*248* (TAG→TAA) 		yhhA
ugpQ		DUF2756 family protein
glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/37);  total (34/37)

AACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGG  >  NC_000913/3587234‑3587497
                                                                                                                                        |                                                                                                                               
aaCACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTa                                                                                                                               <  1:363800/139‑1 (MQ=255)
          gcggGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     <  1:400696/139‑1 (MQ=255)
          gcggGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     <  2:470506/139‑1 (MQ=255)
            gggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTaaa                                                                                                                   >  1:1244943/1‑139 (MQ=255)
             ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     >  2:310001/1‑136 (MQ=255)
             ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     <  1:310001/136‑1 (MQ=255)
             ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAg                                                                                                                  >  1:1211808/1‑139 (MQ=255)
             ggATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAg                                                                                                                  >  1:1217466/1‑139 (MQ=255)
                   tAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGa                                                                                                            <  2:396844/139‑1 (MQ=255)
                      aCAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCa                                                                                                         <  1:1272369/139‑1 (MQ=255)
                        aGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAAt                                                                                                       <  2:1211808/139‑1 (MQ=255)
                         gCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATc                                                                                                      >  1:856181/1‑139 (MQ=255)
                          cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCa                                                                                                     >  2:1324888/1‑139 (MQ=255)
                            aCCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACg                                                                                                   >  2:572650/1‑139 (MQ=255)
                            aCCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACg                                                                                                   >  2:1012714/1‑139 (MQ=255)
                                     cGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCa                                                                                          <  1:296711/139‑1 (MQ=255)
                                        aTAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     >  1:650727/1‑109 (MQ=255)
                                        aTAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     <  2:650727/109‑1 (MQ=255)
                                           gTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTa                                                                                    <  2:335922/139‑1 (MQ=255)
                                            ttCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     <  2:1345800/105‑1 (MQ=255)
                                            ttCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                                                     >  1:1345800/1‑105 (MQ=255)
                                            ttCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTAc                                                                                   >  2:710989/1‑139 (MQ=255)
                                              cGTTGAAGCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCCCGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAg                                                                                 >  2:1083869/1‑139 (MQ=255)
                                               gTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCAt                                                                                         >  2:1365527/1‑130 (MQ=255)
                                               gTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCAt                                                                                         <  1:1365527/130‑1 (MQ=255)
                                                   aaTCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa                                                                            <  2:1217466/139‑1 (MQ=255)
                                                         cagcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCcac                                                                      >  1:1601477/1‑139 (MQ=255)
                                                           gcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCacac                                                                    >  2:902471/1‑139 (MQ=255)
                                                              tatGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACAcccc                                                                 >  1:808726/1‑139 (MQ=255)
                                                                 gctgcCGGAGAGTAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAgc                                                              >  1:646093/1‑139 (MQ=255)
                                                                   tgcCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAgcgc                                                            >  2:520448/1‑139 (MQ=255)
                                                                     ccGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                          <  1:520448/139‑1 (MQ=255)
                                                                      cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                                                                              >  1:919441/1‑102 (MQ=255)
                                                                      cGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTACGGACCAATCACGTCAAt                                                                                              <  2:919441/102‑1 (MQ=255)
                                                                          gaggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAAct                                                     >  1:1567947/1‑139 (MQ=255)
                                                                           aggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTc                                                                                                               <  1:1064253/80‑1 (MQ=255)
                                                                           aggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTc                                                                                                               >  2:1064253/1‑80 (MQ=255)
                                                                            ggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAActct                                                   >  2:1608528/1‑139 (MQ=255)
                                                                               gaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTctgct                                                <  2:1196231/139‑1 (MQ=255)
                                                                                aaaCGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTctgctg                                               <  1:1324888/139‑1 (MQ=255)
                                                                                aaaCGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTctgctg                                               <  2:1004166/139‑1 (MQ=255)
                                                                                    ggCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcg                                           <  1:1083869/139‑1 (MQ=255)
                                                                                    ggCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcg                                           <  1:902471/139‑1 (MQ=255)
                                                                                     gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGC‑ATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                         <  2:1244943/139‑1 (MQ=255)
                                                                                      cGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                         <  2:1509484/139‑1 (MQ=255)
                                                                                      cGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                         <  2:779603/139‑1 (MQ=255)
                                                                                       gACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTg                                        >  2:795683/1‑139 (MQ=255)
                                                                                              tGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGt                                                                                                                      >  1:1039512/1‑54 (MQ=255)
                                                                                              tGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGt                                                                                                                      <  2:1039512/54‑1 (MQ=255)
                                                                                                  tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             <  1:828503/139‑1 (MQ=255)
                                                                                                  tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             <  2:808726/139‑1 (MQ=255)
                                                                                                  tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             <  1:1267852/139‑1 (MQ=255)
                                                                                                  tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             <  2:646093/139‑1 (MQ=255)
                                                                                                     gCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACgg                          >  2:1440355/1‑139 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                                                                              >  2:1635141/1‑70 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAAt                                                                                              <  1:1635141/70‑1 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             >  1:1594316/1‑135 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             <  2:1594316/135‑1 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                         >  2:363241/1‑139 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                         >  2:299543/1‑139 (MQ=255)
                                                                                                      cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGt                         <  2:1380014/139‑1 (MQ=255)
                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATaa                     >  2:667302/1‑139 (MQ=255)
                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATaa                     >  2:830826/1‑139 (MQ=255)
                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGAACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             >  2:1224153/1‑131 (MQ=255)
                                                                                                          gCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGAACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                             <  1:1224153/131‑1 (MQ=255)
                                                                                                                cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                                              <  2:129738/76‑1 (MQ=255)
                                                                                                                cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                                              >  1:129738/1‑76 (MQ=255)
                                                                                                                cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGa               <  2:856181/139‑1 (MQ=255)
                                                                                                                     ccTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCg          <  1:299543/139‑1 (MQ=255)
                                                                                                                          caatCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGAATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTc     >  2:960593/4‑139 (MQ=255)
                                                                                                                             tCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg  <  1:667302/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                               
AACACCAACGGCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGG  >  NC_000913/3587234‑3587497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑