Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,614,092 T→C I142I (ATT→ATC)  nikA → nickel/heme ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,614,0920TC100.0% 163.3 / NA 46I142I (ATT→ATC) nikAnickel/heme ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

CGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGG  >  NC_000913/3613957‑3614220
                                                                                                                                       |                                                                                                                                
cGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGAAGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCAcc                                                                                                                               >  2:996014/1‑139 (MQ=255)
            cGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAgc                                                                                                                      <  1:552455/136‑1 (MQ=255)
            cGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAgc                                                                                                                      >  2:552455/1‑136 (MQ=255)
                gCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCctact                                                                                                               <  2:645439/139‑1 (MQ=255)
                       cggcggcAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCttt                                                                                                        <  2:84259/139‑1 (MQ=255)
                             cAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCa                                                                                                  <  1:198679/139‑1 (MQ=255)
                                  aaCTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATAGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAAc                                                                                             <  2:1186469/139‑1 (MQ=255)
                                                     aTAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCttt                                                                          <  1:1420418/139‑1 (MQ=255)
                                                         ccGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAAc                                                                                             >  2:459557/1‑116 (MQ=255)
                                                         ccGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAAc                                                                                             <  1:459557/116‑1 (MQ=255)
                                                           gTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCttt                                                                                                        <  2:718095/103‑1 (MQ=255)
                                                           gTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCttt                                                                                                        >  1:718095/1‑103 (MQ=255)
                                                                cGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGcc                                                                                        <  2:1475405/114‑1 (MQ=255)
                                                                cGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGcc                                                                                        >  1:1475405/1‑114 (MQ=255)
                                                                           gctggAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAgc                                                                                                                      <  1:1549573/73‑1 (MQ=255)
                                                                           gctggAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAgc                                                                                                                      >  2:1549573/1‑73 (MQ=255)
                                                                                    ggCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTa                                                >  2:311631/1‑134 (MQ=255)
                                                                                    ggCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTa                                                <  1:311631/134‑1 (MQ=255)
                                                                                     gCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGcc                                                                                                                   <  2:1396715/66‑1 (MQ=255)
                                                                                     gCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGcc                                                                                                                   >  1:1396715/1‑66 (MQ=255)
                                                                                     gCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCcta                                                                                                                 <  1:475972/68‑1 (MQ=255)
                                                                                     gCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCcta                                                                                                                 >  2:475972/1‑68 (MQ=255)
                                                                                               ttgttgATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAaaaccatgaaaccat                                >  1:123572/1‑139 (MQ=255)
                                                                                                  ttgAAGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAaccatgaaaccatgaa                             <  1:996014/139‑1 (MQ=255)
                                                                                                     aTGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAgc                                                                                                                      <  2:551369/47‑1 (MQ=255)
                                                                                                     aTGTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAgc                                                                                                                      >  1:551369/1‑47 (MQ=255)
                                                                                                       gTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGcc                                                                                                                   <  1:61572/48‑1 (MQ=255)
                                                                                                       gTTAAAGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGcc                                                                                                                   >  2:61572/1‑48 (MQ=255)
                                                                                                            aGCACTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTaaa                   >  1:1156981/1‑139 (MQ=255)
                                                                                                                cTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAAc                                                                                             <  2:49623/61‑1 (MQ=255)
                                                                                                                cTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAAc                                                                                             >  1:49623/1‑61 (MQ=255)
                                                                                                                cTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGcc                                                                                        >  1:887439/1‑66 (MQ=255)
                                                                                                                cTCAGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGcc                                                                                        <  2:887439/66‑1 (MQ=255)
                                                                                                                   aGTAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGa            >  1:472693/1‑139 (MQ=255)
                                                                                                                     tAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAgcg                <  1:832837/133‑1 (MQ=255)
                                                                                                                     tAAAACAGAGCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAgcg                >  2:832837/1‑133 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGaa                                                                                              <  2:359777/47‑1 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGaa                                                                                              >  1:359777/1‑47 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGcccc                                                                                 <  2:1050387/60‑1 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGcccc                                                                                 >  1:1050387/1‑60 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGAt           >  1:887329/1‑130 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGAt           <  2:887329/130‑1 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCgg  >  2:1643171/1‑139 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCgg  >  1:789233/1‑139 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCgg  >  1:58930/1‑139 (MQ=255)
                                                                                                                             agCTGCAAATCACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCACTCGCAGTTTAAAAACCATGAAACCATGAACAGAATTAAAGCGCCGATTGGCACCgg  >  1:1071187/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                
CGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGG  >  NC_000913/3613957‑3614220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑