Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,615,749	A→G	Y170C (TAC→TGC)	nikB →	nickel ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,615,749	0	A	G	100.0%	191.0 / NA	53	Y170C (TAC→TGC)	nikB	nickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/22);  total (31/22)

GCCATCGCGACCGTCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTACGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTGGGCGCGTCTGC  >  NC_000913/3615614‑3615880                                                                                                                                        |                                                                                                                                    gcCATCGCGACCGTCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGcgg                                                                                                                                  <  2:1513514/139‑1 (MQ=255)            cGTCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGgcagc                                                                                                                       <  2:1576522/139‑1 (MQ=255)              tCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGcac                                                                                                                     <  2:1491243/139‑1 (MQ=255)               cTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTgcggcg                                                                                                                                >  2:140380/1‑127 (MQ=255)               cTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTgcggcg                                                                                                                                <  1:140380/127‑1 (MQ=255)               cTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGgc                                                                                                                          >  2:630398/1‑133 (MQ=255)               cTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGgc                                                                                                                          <  1:630398/133‑1 (MQ=255)                               tACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGt                                                                                                    >  1:551308/1‑139 (MQ=255)                                aCGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTAGCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGtt                                                                                                   >  1:901196/1‑139 (MQ=255)                                aCGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTAGCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGtt                                                                                                   <  2:901196/139‑1 (MQ=255)                                    ttCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               <  1:646150/139‑1 (MQ=255)                                     tCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCAt                                                                                              <  2:86848/139‑1 (MQ=255)                                       aTCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               >  1:246139/1‑136 (MQ=255)                                       aTCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               <  2:246139/136‑1 (MQ=255)                                         cgcgTTTCTTGGCGTGTCGATGCCTAACGTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTGTCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGcc                                                                                          >  1:1027243/1‑139 (MQ=255)                                           cgTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCtt                                                                                        >  1:1265525/1‑139 (MQ=255)                                                         tCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGa                                                                          >  1:517797/1‑139 (MQ=255)                                                                         ggctggcGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACt                                                          >  1:117382/1‑139 (MQ=255)                                                                         ggctggcGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACt                                                          <  2:459970/139‑1 (MQ=255)                                                                          gctggcGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTg                                                         <  2:1435820/139‑1 (MQ=255)                                                                                         ggTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTg                                          >  2:473282/1‑139 (MQ=255)                                                                                          gTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTaa                                                                      <  2:365672/110‑1 (MQ=255)                                                                                          gTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTaa                                                                      >  1:365672/1‑110 (MQ=255)                                                                                          gTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTgg                                         >  1:1081610/1‑139 (MQ=255)                                                                                            gATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCAt                                                                                              >  2:228217/1‑84 (MQ=255)                                                                                            gATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCAt                                                                                              <  1:228217/84‑1 (MQ=255)                                                                                               ggCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCa                                                  >  1:705253/1‑125 (MQ=255)                                                                                               ggCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCa                                                  <  2:705253/125‑1 (MQ=255)                                                                                               ggCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTc                                    >  2:544558/1‑139 (MQ=255)                                                                                                    tttCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGcgg                                                                                                                                  >  1:10799/1‑40 (MQ=255)                                                                                                    tttCGGTGTATCTGCAATGGCTACCCGCGATGGGTTGcgg                                                                                                                                  <  2:10799/40‑1 (MQ=255)                                                                                                          tgtATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGcg                         >  2:429602/1‑139 (MQ=255)                                                                                                           gtATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGgca                                                                                                                         >  1:400114/1‑42 (MQ=255)                                                                                                           gtATCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGgca                                                                                                                         <  2:400114/42‑1 (MQ=255)                                                                                                              tCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTg                                                         >  2:234934/1‑103 (MQ=255)                                                                                                              tCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTg                                                         <  1:234934/103‑1 (MQ=255)                                                                                                              tCTGCAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGcgtcac                     >  2:863780/1‑139 (MQ=255)                                                                                                                  cAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCgg                                                                                                     >  2:669057/1‑55 (MQ=255)                                                                                                                  cAATGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCgg                                                                                                     <  1:669057/55‑1 (MQ=255)                                                                                                                   aaTGGCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCgtcacgtcac                >  1:904307/1‑139 (MQ=255)                                                                                                                      ggCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCAt                                                                                              <  2:192049/58‑1 (MQ=255)                                                                                                                      ggCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCAt                                                                                              >  1:192049/1‑58 (MQ=255)                                                                                                                      ggCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTg             >  1:1270205/1‑139 (MQ=255)                                                                                                                      ggCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTg             >  1:546369/1‑139 (MQ=255)                                                                                                                       gCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAAcg                                                                    >  1:1343875/1‑83 (MQ=255)                                                                                                                       gCTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAAcg                                                                    <  2:1343875/83‑1 (MQ=255)                                                                                                                        cTACCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTggg           >  1:771233/1‑139 (MQ=255)                                                                                                                          aCCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGcc                                                   <  2:977912/97‑1 (MQ=255)                                                                                                                          aCCCGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGcc                                                   >  1:977912/1‑97 (MQ=255)                                                                                                                            ccGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               >  2:792371/1‑51 (MQ=255)                                                                                                                            ccGCGATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               <  1:792371/51‑1 (MQ=255)                                                                                                                               cgATGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTGGGCGCGTCt    >  2:106430/1‑139 (MQ=255)                                                                                                                                 aTGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               <  2:562077/46‑1 (MQ=255)                                                                                                                                 aTGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCa                                                                                               >  1:562077/1‑46 (MQ=255)                                                                                                                                 aTGGGTTGCGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTGGGCGCGTCTgc  >  1:1550659/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    GCCATCGCGACCGTCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTACGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTGGGCGCGTCTGC  >  NC_000913/3615614‑3615880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑