Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,855,960	C→T	S4N (AGC→AAC)  *116* (TAG→TAA)	yidG ← yidH ←	inner membrane protein DUF202 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,855,960	0	C	T	100.0%	214.2 / NA	66	S4N (AGC→AAC)  *116* (TAG→TAA)	yidG yidH	inner membrane protein DUF202 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/40);  total (26/40)

ACATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCA  >  NC_000913/3855826‑3856093                                                                                                                                       |                                                                                                                                      aCATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCATCGCGCCGTAGCCCAGCACGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                   <  2:1065034/139‑1 (MQ=255) aCATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                   <  2:855930/139‑1 (MQ=255) aCATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGGTCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                   <  1:123942/139‑1 (MQ=255)  cATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATcc                                                                                                                                  >  1:781430/1‑139 (MQ=255)    tGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCgg                                                                                                                                <  1:141592/139‑1 (MQ=255)       ccGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                                 >  1:47970/1‑135 (MQ=255)       ccGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                                 <  2:47970/135‑1 (MQ=255)              gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      <  1:721655/139‑1 (MQ=255)              gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      <  2:149881/139‑1 (MQ=255)              gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      <  1:942407/139‑1 (MQ=255)                 ccAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAg                                                                                                                   <  2:781430/139‑1 (MQ=255)                 ccAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAg                                                                                                                   <  1:740686/139‑1 (MQ=255)                     ttGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTccc                                                                                                               <  2:139982/139‑1 (MQ=255)                         gtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATaa                                                                                                           >  1:518504/1‑139 (MQ=255)                          tttGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAAc                                                                                                          >  2:254793/1‑139 (MQ=255)                               tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      <  1:856598/122‑1 (MQ=255)                               tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      >  2:856598/1‑122 (MQ=255)                                  ccAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCg                                                                                                  >  2:979849/1‑139 (MQ=255)                                       gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             <  1:3594/139‑1 (MQ=255)                                       gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             <  2:820300/139‑1 (MQ=255)                                       gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             <  1:597600/139‑1 (MQ=255)                                            cAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                        <  1:624392/139‑1 (MQ=255)                                               cgcgCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATa                                                                                                            <  2:555835/116‑1 (MQ=255)                                               cgcgCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATa                                                                                                            >  1:555835/1‑116 (MQ=255)                                                 cgcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAata                                                                                   >  2:1223689/1‑139 (MQ=255)                                                  gcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      <  2:1475497/103‑1 (MQ=255)                                                  gcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAacac                                                                                                                      >  1:1475497/1‑103 (MQ=255)                                                   ccGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATAtt                                                                                 >  1:390053/1‑139 (MQ=255)                                                   ccGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATAtt                                                                                 <  1:152507/139‑1 (MQ=255)                                                                   tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATcc                                                                                                                                  >  1:549549/1‑74 (MQ=255)                                                                   tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATcc                                                                                                                                  <  2:549549/74‑1 (MQ=255)                                                                   tACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCACTATTAAGCTAATGATtaata                                                                 <  2:972150/139‑1 (MQ=255)                                                                     cGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAg                                                               <  1:1223689/139‑1 (MQ=255)                                                                         aCCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                           <  2:518504/139‑1 (MQ=255)                                                                           cACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             <  2:686641/103‑1 (MQ=255)                                                                           cACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             >  1:686641/1‑103 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                   >  1:211767/1‑63 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATc                                                                                                                                   <  2:211767/63‑1 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                                 >  1:1217316/1‑65 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCg                                                                                                                                 <  2:1217316/65‑1 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGgt                                                       >  2:1570022/1‑139 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGgt                                                       >  1:1566373/1‑139 (MQ=255)                                                                             cGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGgt                                                       >  1:916821/1‑139 (MQ=255)                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             <  2:1321322/90‑1 (MQ=255)                                                                                        tgCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             >  1:1321322/1‑90 (MQ=255)                                                                                           gcTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCct                                         <  2:1182418/139‑1 (MQ=255)                                                                                            cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCt                                                                            <  1:1450200/103‑1 (MQ=255)                                                                                            cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCt                                                                            >  2:1450200/1‑103 (MQ=255)                                                                                            cTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCctc                                        >  2:255000/1‑139 (MQ=255)                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                           <  2:221474/117‑1 (MQ=255)                                                                                               cGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                           >  1:221474/1‑117 (MQ=255)                                                                                                           tGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                           <  2:278840/105‑1 (MQ=255)                                                                                                           tGGGTCGGCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGt                                                           >  1:278840/1‑105 (MQ=255)                                                                                                                 ggCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTc                   <  2:1566373/139‑1 (MQ=255)                                                                                                                 ggCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTc                   <  1:255000/139‑1 (MQ=255)                                                                                                                 ggCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTc                   <  1:979849/139‑1 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             <  1:1151985/64‑1 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACg                                                                                             >  2:1151985/1‑64 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                        <  2:894827/69‑1 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt                                                                                        >  1:894827/1‑69 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTc                                           >  1:1076746/1‑114 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTc                                           <  2:1076746/114‑1 (MQ=255)                                                                                                                  gCAATGCGTCTGGCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCa                  >  1:1417673/1‑139 (MQ=255)                                                                                                                              gCTTTGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAg      <  1:1570022/139‑1 (MQ=255)                                                                                                                                  tGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCa  <  2:390053/139‑1 (MQ=255)                                                                                                                                  tGCGGTTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCa  <  1:254793/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ACATGCCCGCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATATTAAGCTAATGATTAATAAGCTGTTGGTGTAGGGCAGGTCCTCTTTCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCA  >  NC_000913/3855826‑3856093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑