Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,441 G→A *355* (TAG→TAA) 
G430G (GGC→GGT) 		 cbrA →
dgoT ←		 colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,4410GA97.1% 217.9 / 0.8 69*355* (TAG→TAA) 
G430G (GGC→GGT) 		cbrA
dgoT		colicin M resistance protein, FAD‑binding protein, putative oxidoreductase
D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/33);  minor base C (1/1);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

TTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAAC  >  NC_000913/3870306‑3870569
                                                                                                                                       |                                                                                                                                
ttACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCa                                                                                                                               <  1:512503/139‑1 (MQ=255)
  aCTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                             <  2:1092439/139‑1 (MQ=255)
      gcgcgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACgcgc                                                                                                                         >  1:1126006/1‑139 (MQ=255)
          gcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                     >  1:428539/1‑139 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  2:445161/139‑1 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      >  1:325717/1‑139 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  2:325717/139‑1 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  1:651623/139‑1 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      >  2:1598704/1‑139 (MQ=255)
                         cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  2:1126006/139‑1 (MQ=255)
                                               tAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  1:417596/117‑1 (MQ=255)
                                               tAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      >  2:417596/1‑117 (MQ=255)
                                                        gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                        >  1:203581/1‑106 (MQ=255)
                                                        gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                        <  2:203581/106‑1 (MQ=255)
                                                        gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAc                                                                       >  2:134524/1‑139 (MQ=255)
                                                        gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAc                                                                       >  2:444838/1‑139 (MQ=255)
                                                         ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTACCCAACGCGCTTCACATCGCCCACcag                                                                                                        >  2:12305/1‑105 (MQ=255)
                                                         ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTACCCAACGCGCTTCACATCGCCCACcag                                                                                                        <  1:12305/105‑1 (MQ=255)
                                                         ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcg                                                                      >  1:996043/1‑139 (MQ=255)
                                                            aTGCCTGACCGCACCGGGTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                             <  2:1075807/81‑1 (MQ=255)
                                                            aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAATGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  2:874869/104‑1 (MQ=255)
                                                            aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                             >  1:1075807/1‑81 (MQ=255)
                                                            aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      >  1:874869/1‑104 (MQ=255)
                                                             tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  <  2:428539/139‑1 (MQ=255)
                                                             tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  <  1:1659925/139‑1 (MQ=255)
                                                                 tGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGat                                                              <  1:820239/139‑1 (MQ=255)
                                                                   aCCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                              <  1:1433029/89‑1 (MQ=255)
                                                                   aCCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                              >  2:1433029/1‑89 (MQ=255)
                                                                    ccGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                            >  1:550270/1‑74 (MQ=255)
                                                                    ccGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                            <  2:550270/74‑1 (MQ=255)
                                                                           ggCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGt                                                    >  1:137516/1‑139 (MQ=255)
                                                                                     aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                          <  2:137516/139‑1 (MQ=255)
                                                                                      aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaa                                         >  1:866760/1‑139 (MQ=255)
                                                                                       gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaaa                                        >  1:1222269/1‑139 (MQ=255)
                                                                                              aTGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                  <  2:621658/58‑1 (MQ=255)
                                                                                              aTGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACAt                                                                                                                  >  1:621658/1‑58 (MQ=255)
                                                                                                 cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCt                              <  2:1222269/139‑1 (MQ=255)
                                                                                                  gcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTGACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGTAGGTGCGAAACCGTAACCCTg                             <  2:996043/139‑1 (MQ=255)
                                                                                                     gtggtgTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                          <  2:1125534/123‑1 (MQ=255)
                                                                                                     gtgatgTGGCACATATTCCAGAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCgt                                          >  1:1125534/1‑122 (MQ=255)
                                                                                                         tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAgg                      >  1:703685/1‑139 (MQ=255)
                                                                                                         tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAgg                      <  2:1654305/139‑1 (MQ=255)
                                                                                                          gtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGt                     <  1:1598704/139‑1 (MQ=255)
                                                                                                                caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                          <  2:818766/112‑1 (MQ=255)
                                                                                                                caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCcca               >  2:1347647/1‑139 (MQ=255)
                                                                                                                caTATTCCACAGTTGAAGGATTAACCAACGCGCTTAACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                          >  1:818766/1‑112 (MQ=255)
                                                                                                                 atatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccac              <  1:1347647/139‑1 (MQ=255)
                                                                                                                  tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  >  2:785108/1‑86 (MQ=255)
                                                                                                                  tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  <  1:785108/86‑1 (MQ=255)
                                                                                                                   atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  <  1:1019157/85‑1 (MQ=255)
                                                                                                                   atTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  >  2:1019157/1‑85 (MQ=255)
                                                                                                                           gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  <  1:361450/77‑1 (MQ=255)
                                                                                                                           gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  >  2:361450/1‑77 (MQ=255)
                                                                                                                           gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGa    >  1:1594352/1‑139 (MQ=255)
                                                                                                                            ttGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAAt                                                                                >  1:957751/1‑62 (MQ=255)
                                                                                                                            ttGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAAt                                                                                <  2:957751/62‑1 (MQ=255)
                                                                                                                            ttGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGaa   >  2:565043/1‑139 (MQ=255)
                                                                                                                             tGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAAc  >  2:1639218/1‑139 (MQ=255)
                                                                                                                             tGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAAc  >  1:1567258/1‑139 (MQ=255)
                                                                                                                              gAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAgg                      <  2:1510072/118‑1 (MQ=255)
                                                                                                                              gAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAgg                      >  1:1510072/1‑118 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      >  2:1512262/1‑37 (MQ=38)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                      <  1:1512262/37‑1 (MQ=38)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                    >  2:359177/1‑39 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                    <  1:359177/39‑1 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  >  1:1347271/1‑73 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                  <  2:1347271/73‑1 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTgcg                           <  2:523160/112‑1 (MQ=255)
                                                                                                                               aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTgcg                           >  1:523160/1‑112 (MQ=255)
                                                                                                                                       |                                                                                                                                
TTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAAC  >  NC_000913/3870306‑3870569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑