Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,908,514	A→G	intergenic (‑148/+35)	pstB ← / ← pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,908,514	0	A	G	100.0%	177.8 / NA	50	intergenic (‑148/+35)	pstB/pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

CATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCC  >  NC_000913/3908381‑3908633                                                                                                                                      |                                                                                                                        cATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGtt                                                                                                                    <  1:467775/139‑1 (MQ=255)    cGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAgg                                                                                                                 >  2:118327/1‑139 (MQ=255)      tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt                                                                                                               <  1:481208/139‑1 (MQ=255)        ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGa                                                                                                             >  2:765934/1‑139 (MQ=255)        ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGa                                                                                                             >  1:1153161/1‑139 (MQ=255)                 cTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAAc                                                                                                    >  1:887621/1‑139 (MQ=255)                  ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAAcc                                                                                                   >  2:724083/1‑139 (MQ=255)                   tCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCg                                                                                                  >  1:1102103/1‑139 (MQ=255)                    cGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgt                                                                                                 >  2:1066333/1‑139 (MQ=255)                          tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                           <  1:907757/139‑1 (MQ=255)                          tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                           <  2:1474125/139‑1 (MQ=255)                          tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                           <  1:1475068/139‑1 (MQ=255)                              gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAata                                                                                       >  2:12949/1‑139 (MQ=255)                                ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATc                                                                                     <  2:1153161/139‑1 (MQ=255)                                   ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAGAGTGTTAACCGTGTACAGAATCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                  >  2:1143928/1‑139 (MQ=255)                                   ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgt                                                                                                 >  1:1630514/1‑124 (MQ=255)                                   ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCgt                                                                                                 <  2:1630514/124‑1 (MQ=255)                                   ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                  <  2:887621/139‑1 (MQ=255)                                   ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                  <  2:1393476/139‑1 (MQ=255)                                              aTCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                       <  1:224781/139‑1 (MQ=255)                                                     gTTCACGCCGCATCCCGCAAAGTGTTAACCGTGTACAGAGTCAAAAACGCGCTCGGTCGGTCCTCTCGCCCCTCTGGGGAGGGGGATAGGGAGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa                                                                >  2:510611/1‑139 (MQ=39)                                                        cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGa                                                                                                             >  1:718680/1‑91 (MQ=255)                                                        cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGa                                                                                                             <  2:718680/91‑1 (MQ=255)                                                        cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAg                                                                                            <  2:1365610/108‑1 (MQ=255)                                                        cACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAg                                                                                            >  1:1365610/1‑108 (MQ=255)                                                             cgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                           >  2:1009186/1‑104 (MQ=255)                                                             cgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCATCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                           <  1:1009186/104‑1 (MQ=255)                                                                                aCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagca                                     <  1:12949/139‑1 (MQ=255)                                                                                aCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagca                                     <  2:1542110/139‑1 (MQ=255)                                                                                aCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagca                                     <  1:118327/139‑1 (MQ=255)                                                                                     gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                          >  1:797803/1‑129 (MQ=255)                                                                                     gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                          <  2:797803/129‑1 (MQ=255)                                                                                     gtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGt                                <  1:1286464/139‑1 (MQ=255)                                                                                          gagTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca                           <  1:775760/139‑1 (MQ=255)                                                                                          gaatCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca                           <  1:1143928/136‑1 (MQ=255)                                                                                           agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGG‑AAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                         >  1:542879/1‑139 (MQ=255)                                                                                           agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGAAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                       >  2:1243577/1‑94 (MQ=255)                                                                                           agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGAAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                       <  1:1243577/94‑1 (MQ=255)                                                                                               aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt                                                                                                               >  2:1115849/1‑50 (MQ=255)                                                                                               aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGt                                                                                                               <  1:1115849/50‑1 (MQ=255)                                                                                                aaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGt                     >  1:23265/1‑139 (MQ=255)                                                                                                 aaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACg                             >  1:257704/1‑130 (MQ=255)                                                                                                 aaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACg                             <  2:257704/130‑1 (MQ=255)                                                                                                 aaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTa                    <  1:765934/139‑1 (MQ=255)                                                                                                       gcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTa                    <  2:1340589/133‑1 (MQ=255)                                                                                                       gcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTa                    >  1:1340589/1‑133 (MQ=255)                                                                                                             tcggtcCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGG‑AAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAccc       <  2:542879/139‑1 (MQ=255)                                                                                                                   cccTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc  <  2:1102103/139‑1 (MQ=255)                                                                                                                           ccTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                  <  2:657237/51‑1 (MQ=255)                                                                                                                           ccTCTGGGGAGGGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                  >  1:657237/1‑51 (MQ=255)                                                                                                                                      |                                                                                                                        CATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCC  >  NC_000913/3908381‑3908633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑