Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,920,950	C→T	*80* (TAG→TAA)	atpE ←	F0 sector of membrane‑bound ATP synthase, subunit c

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,920,950	0	C	T	100.0%	232.8 / NA	71	*80* (TAG→TAA)	atpE	F0 sector of membrane‑bound ATP synthase, subunit c
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/31);  total (40/31)

GCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCC  >  NC_000913/3920826‑3921082                                                                                                                             |                                                                                                                                     gCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAg                                                                                                                            >  1:1140403/1‑135 (MQ=255) gCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAg                                                                                                                            <  2:1140403/135‑1 (MQ=255)     aacagaacGAACAGGACAAAGGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACagc                                                                                                                    >  2:972386/1‑137 (MQ=255)           acgaacAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc                                                                                                              <  1:586406/139‑1 (MQ=255)               acAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagac                                                                                                          >  1:86270/1‑139 (MQ=255)                   gACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCttatta                                                                                                                                    <  1:1639851/109‑1 (MQ=255)                    aCAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATc                                                                                                                    >  2:1367107/1‑124 (MQ=255)                    aCAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATc                                                                                                                    <  1:1367107/124‑1 (MQ=255)                         cgcgATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTAc                                                                                                >  1:21131/1‑139 (MQ=255)                             aTGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc                                                                                                              >  2:911226/1‑121 (MQ=255)                             aTGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAc                                                                                                              <  1:911226/121‑1 (MQ=255)                                          ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTATGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAg                                                                               >  2:1487385/1‑139 (MQ=255)                                          ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                  >  1:616606/1‑136 (MQ=255)                                          ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                  <  2:616606/136‑1 (MQ=255)                                          ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAg                                                                               >  1:1058358/1‑139 (MQ=255)                                          ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAg                                                                               >  1:393885/1‑139 (MQ=255)                                          ggATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAg                                                                               >  2:822807/1‑139 (MQ=255)                                               gttgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATcc                                                                          >  1:543343/1‑139 (MQ=255)                                                 tgCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccac                                                                        <  2:292672/139‑1 (MQ=255)                                                            tCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTATGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATaa                                                             <  1:1487385/139‑1 (MQ=255)                                                                 gCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATa                                                        <  1:822807/139‑1 (MQ=255)                                                                      aTGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGaa                                                   <  2:543343/139‑1 (MQ=255)                                                                       tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACg                                                                                                                 <  1:827861/76‑1 (MQ=255)                                                                       tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACg                                                                                                                 >  2:827861/1‑76 (MQ=255)                                                                       tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc                                                                       >  1:162551/1‑118 (MQ=255)                                                                       tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccacc                                                                       <  2:162551/118‑1 (MQ=255)                                                                       tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccac                                                                        >  1:923080/1‑117 (MQ=255)                                                                       tGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATccac                                                                        <  2:923080/117‑1 (MQ=255)                                                                       tGCCTCTATTTAGTT‑ACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcgcg                                                                                                                                >  1:143922/1‑60 (MQ=255)                                                                       tGCCTCTATTTAGTT‑ACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTAcgcg                                                                                                                                <  2:143922/60‑1 (MQ=255)                                                                        gCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                 <  2:393885/139‑1 (MQ=255)                                                                        gCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                 <  2:42070/139‑1 (MQ=255)                                                                            ctATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGt                                             >  1:652958/1‑139 (MQ=255)                                                                               tttAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                          <  2:21131/139‑1 (MQ=255)                                                                               tttAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                          <  2:652958/139‑1 (MQ=255)                                                                                  aGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATaa                                                             <  2:246310/117‑1 (MQ=255)                                                                                  aGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATaa                                                             >  1:246310/1‑117 (MQ=255)                                                                                   gTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGATTATTACTAGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATAATCGAGATAGCATCCACCAGACCCATAAAGATAAAGAACTGAGTACgcagc                                      >  2:1398281/1‑139 (MQ=255)                                                                                    ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCa                                                                                                                   <  1:421548/61‑1 (MQ=255)                                                                                    ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCa                                                                                                                   >  2:421548/1‑61 (MQ=255)                                                                                    ttAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACgcagca                                     <  2:1058358/139‑1 (MQ=255)                                                                                      aaCGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                   <  2:86270/139‑1 (MQ=255)                                                                                            cTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                  <  2:1510456/86‑1 (MQ=255)                                                                                            cTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGa                                                                                  >  1:1510456/1‑86 (MQ=255)                                                                                            cTGATATTGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAatc                             >  1:339493/1‑139 (MQ=255)                                                                                                  ttGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATaa                                                             <  2:1572941/101‑1 (MQ=255)                                                                                                  ttGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATaa                                                             >  1:1572941/1‑101 (MQ=255)                                                                                                   tGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAatcagatcag                      >  2:110405/1‑139 (MQ=255)                                                                                                   tGCTCTTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAatcagatcag                      >  1:152309/1‑139 (MQ=255)                                                                                                     ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                    >  1:313941/1‑139 (MQ=255)                                                                                                     ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                    >  1:131844/1‑139 (MQ=255)                                                                                                     ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                    >  1:133891/1‑139 (MQ=255)                                                                                                     ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                    >  1:1423921/1‑139 (MQ=255)                                                                                                     ctctTTAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGt                    >  1:1567399/1‑139 (MQ=255)                                                                                                         ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc                                                                                                         <  1:664421/50‑1 (MQ=255)                                                                                                         ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTAcagacc                                                                                                         >  2:664421/1‑50 (MQ=255)                                                                                                         ttAAATAAAAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGAc                >  2:666515/1‑139 (MQ=255)                                                                                                          taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                             <  2:1184872/77‑1 (MQ=255)                                                                                                          taaataaaAGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCa                                                                             >  1:1184872/1‑77 (MQ=255)                                                                                                                 aaGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcg        <  2:907658/139‑1 (MQ=255)                                                                                                                  aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                          <  2:237205/104‑1 (MQ=255)                                                                                                                  aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACg                                          >  1:237205/1‑104 (MQ=255)                                                                                                                  aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                   >  1:293786/1‑111 (MQ=255)                                                                                                                  aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCaga                                   <  2:293786/111‑1 (MQ=255)                                                                                                                  aGCAACGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTgcgc       >  2:927988/1‑139 (MQ=255)                                                                                                                      aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                 <  1:367441/93‑1 (MQ=255)                                                                                                                      aCGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACt                                                 >  2:367441/1‑93 (MQ=255)                                                                                                                       cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATa                                                                                >  2:1278651/1‑61 (MQ=255)                                                                                                                       cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATa                                                                                <  1:1278651/61‑1 (MQ=255)                                                                                                                       cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGa                                >  1:201668/1‑109 (MQ=255)                                                                                                                       cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGa                                <  2:201668/109‑1 (MQ=255)                                                                                                                       cGCTTATTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTcc  >  1:474896/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                     GCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCGACAGCGAACATCACGTACAGACCCAGACCTACAGCGATCATCGGGATAGCATCCACCAGACCCATAACGATAAAGAACTGAGTACGCAGCAGAGGAATCAGATCAGGTTGACGCGCTGCGCCTTCC  >  NC_000913/3920826‑3921082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑