Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,054	C→T	P301L (CCA→CTA)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,054	0	C	T	100.0%	233.6 / NA	71	P301L (CCA→CTA)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/37);  total (34/37)

ATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCT  >  NC_000913/3991919‑3992179                                                                                                                                        |                                                                                                                              aTTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCtata                                                                                                                            >  1:1225724/1‑139 (MQ=255)      ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       <  1:1533685/139‑1 (MQ=255)      ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       <  2:896154/139‑1 (MQ=255)              aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       >  1:20892/1‑131 (MQ=255)              aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       <  2:20892/131‑1 (MQ=255)                cGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                             >  2:524062/1‑139 (MQ=255)                             acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                                <  1:1230410/139‑1 (MQ=255)                             acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                                <  2:526629/139‑1 (MQ=255)                               tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATa                                                                                              >  1:108161/1‑139 (MQ=255)                               tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATa                                                                                              >  1:989231/1‑139 (MQ=255)                               tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATa                                                                                              >  1:1224663/1‑139 (MQ=255)                                gctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATAc                                                                                             >  2:153571/1‑139 (MQ=255)                                     tgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGAc                                                                                        <  1:1434099/139‑1 (MQ=255)                                       gAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACgg                                                                                      <  2:519964/139‑1 (MQ=255)                                         aGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCg                                                                                    >  2:1471510/1‑139 (MQ=255)                                            cTATTCCTGGTAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTAACGTCTGGTCTCGATCTATACTGCATGATGCTg                                                                                                               >  2:117939/1‑109 (MQ=255)                                            cTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                               <  1:117939/109‑1 (MQ=255)                                             tATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                                <  2:989231/139‑1 (MQ=255)                                                    gggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       <  1:1537392/93‑1 (MQ=255)                                                    gggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       >  2:1537392/1‑93 (MQ=255)                                                     ggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGAtttt                                                                        >  2:900207/1‑139 (MQ=255)                                                      gAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTa                                                                       <  2:199263/139‑1 (MQ=255)                                                         tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGTTCTATACTACa                                                                                                                       <  2:960851/88‑1 (MQ=255)                                                         tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       >  1:960851/1‑88 (MQ=255)                                                         tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAccc                                                                    >  1:702308/1‑139 (MQ=255)                                                          aCCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCg                                                                   >  1:1174045/1‑139 (MQ=255)                                                           cccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                  >  2:722628/1‑139 (MQ=255)                                                            ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                 <  2:237178/139‑1 (MQ=255)                                                            ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                 <  1:917873/139‑1 (MQ=255)                                                            ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                 >  1:742950/1‑139 (MQ=255)                                                             cGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                                <  2:702308/139‑1 (MQ=255)                                                               aaCCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                               >  2:1366653/1‑90 (MQ=255)                                                               aaCCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                               <  1:1366653/90‑1 (MQ=255)                                                                 cccACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGTACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                        <  2:1339297/95‑1 (MQ=255)                                                                 cccACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGTACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                        >  1:1339297/1‑95 (MQ=255)                                                                    aCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                        >  1:1443192/1‑92 (MQ=255)                                                                    aCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                        <  2:1443192/92‑1 (MQ=255)                                                                     cGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAg                                                        >  2:1469665/1‑139 (MQ=255)                                                                         tgctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACg                                                    >  2:1292946/1‑139 (MQ=255)                                                                          gctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGt                                                   <  2:1225724/139‑1 (MQ=255)                                                                          gctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGt                                                   >  1:703076/1‑139 (MQ=255)                                                                           ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                     <  1:906943/88‑1 (MQ=255)                                                                           ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                     >  2:906943/1‑88 (MQ=255)                                                                           ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTc                                                  <  2:1224663/139‑1 (MQ=255)                                                                            tgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCg                                                 <  2:1360488/139‑1 (MQ=255)                                                                              gCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGT‑CGTCgctg                                              >  2:668062/1‑139 (MQ=255)                                                                                gAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgctgc                                             <  1:900207/139‑1 (MQ=255)                                                                                  aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                           <  1:1292946/139‑1 (MQ=255)                                                                                  aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                           <  1:1495775/139‑1 (MQ=255)                                                                                  aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                           <  1:1471510/139‑1 (MQ=255)                                                                                        tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       <  1:495665/57‑1 (MQ=255)                                                                                        tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       <  1:1174164/57‑1 (MQ=255)                                                                                        tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                       >  2:495665/1‑57 (MQ=255)                                                                                        tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGt                                                       <  2:141949/121‑1 (MQ=255)                                                                                        tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGt                                                       >  1:141949/1‑121 (MQ=255)                                                                                        tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCAATCTATACTGCa                                                                                                                       >  2:1174164/1‑57 (MQ=255)                                                                                                  tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                  <  1:813404/100‑1 (MQ=255)                                                                                                  tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                  >  2:813404/1‑100 (MQ=255)                                                                                                         gacgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGc                    <  2:292518/139‑1 (MQ=255)                                                                                                          acgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCt                   >  1:692456/1‑139 (MQ=255)                                                                                                          acgGCGAGATCGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCt                   >  1:77860/1‑139 (MQ=255)                                                                                                              cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                     <  1:732123/53‑1 (MQ=255)                                                                                                              cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                     >  2:732123/1‑53 (MQ=255)                                                                                                                   ttGTATCGTTTGGTCTCGATCTATAC‑GCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGaa         <  2:747960/139‑1 (MQ=255)                                                                                                                         cGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACgcgc    <  1:1469665/139‑1 (MQ=255)                                                                                                                           tttGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                       >  2:1452395/1‑38 (MQ=255)                                                                                                                           tttGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                       <  1:1452395/38‑1 (MQ=255)                                                                                                                           tttGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCt  >  2:858990/1‑139 (MQ=255)                                                                                                                                tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                                >  1:98921/1‑56 (MQ=255)                                                                                                                                tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                                <  2:98921/56‑1 (MQ=255)                                                                                                                                     aTCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                           >  2:392638/1‑88 (MQ=255)                                                                                                                                     aTCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                           <  1:392638/88‑1 (MQ=255)                                                                                                                                        |                                                                                                                              ATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCT  >  NC_000913/3991919‑3992179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑