Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,158,592 C→T Q35* (CAA→TAA)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,5920CT100.0% 282.6 / NA 86Q35* (CAA→TAA) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/44);  total (42/44)

CGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTC  >  NC_000913/4158456‑4158720
                                                                                                                                        |                                                                                                                                
cGCCATGAACTATCGTGGCGGTGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTaa                                                                                                                                >  1:360822/1‑139 (MQ=255)
cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTaa                                                                                                                                >  2:1045998/1‑139 (MQ=255)
     tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCg                                                                                                                           >  1:1073630/1‑139 (MQ=255)
     tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCg                                                                                                                           >  1:720528/1‑139 (MQ=255)
      gAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGt                                                                                                                          <  2:385018/139‑1 (MQ=255)
      gAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGt                                                                                                                          <  1:1361101/139‑1 (MQ=255)
                 gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGa                                                                                                               >  2:726908/1‑139 (MQ=255)
                 gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGa                                                                                                               >  2:1037693/1‑139 (MQ=255)
                      ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGc                                                                                                          >  1:42986/1‑139 (MQ=255)
                       gaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCt                                                                                                         <  1:784873/139‑1 (MQ=255)
                          gatggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTggg                                                                                                      <  2:248847/139‑1 (MQ=255)
                               atAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                                                 <  2:1073630/139‑1 (MQ=255)
                                     aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGc                                                                                           >  2:1196809/1‑139 (MQ=255)
                                        atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCt                                                                                                                     >  2:867334/1‑110 (MQ=255)
                                        atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCt                                                                                                                     <  1:867334/110‑1 (MQ=255)
                                        atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTgg                                                                                        >  2:1542070/1‑139 (MQ=255)
                                          tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      >  1:159578/1‑139 (MQ=255)
                                          tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      >  2:372696/1‑139 (MQ=255)
                                          tCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      >  1:267709/1‑139 (MQ=255)
                                             tGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCgg                                                                                   <  2:106221/139‑1 (MQ=255)
                                             tGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATACGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCgg                                                                                   <  1:1080696/139‑1 (MQ=255)
                                               aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCt                                                                                                                     <  2:1655650/103‑1 (MQ=255)
                                               aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCt                                                                                                                     >  1:1655650/1‑103 (MQ=255)
                                               aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 <  2:720528/139‑1 (MQ=255)
                                               aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 >  2:1554832/1‑139 (MQ=255)
                                                tCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAcc                                                                                <  1:1036606/139‑1 (MQ=255)
                                                  ttGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                                              <  1:1316024/139‑1 (MQ=255)
                                                       tACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                                                                         <  1:254465/139‑1 (MQ=255)
                                                           tggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGc                                                                                                          <  1:345220/102‑1 (MQ=255)
                                                           tggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGc                                                                                                          >  2:345220/1‑102 (MQ=255)
                                                            ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTa                                                                                                                         >  2:1234031/1‑86 (MQ=255)
                                                            ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTa                                                                                                                         <  1:1234031/86‑1 (MQ=255)
                                                            ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                    >  2:837424/1‑139 (MQ=255)
                                                            ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                    >  1:885962/1‑139 (MQ=255)
                                                            ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                    >  2:873153/1‑139 (MQ=255)
                                                               ggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttg                                                                 >  2:277745/1‑139 (MQ=255)
                                                                   ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 >  2:606365/1‑119 (MQ=255)
                                                                   ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 <  1:606365/119‑1 (MQ=255)
                                                                    tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTggg                                                                                                      <  1:204196/97‑1 (MQ=255)
                                                                    tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTggg                                                                                                      >  2:204196/1‑97 (MQ=255)
                                                                    tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGt                                                                                                   >  1:1542525/1‑100 (MQ=255)
                                                                    tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGt                                                                                                   <  2:1542525/100‑1 (MQ=255)
                                                                    tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                                            >  2:349152/1‑139 (MQ=255)
                                                                     ggCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAcc                                                           <  1:349152/139‑1 (MQ=255)
                                                                      gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                     >  2:1437809/1‑96 (MQ=255)
                                                                      gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                                                     <  1:1437809/96‑1 (MQ=255)
                                                                          aaCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 >  1:132453/1‑112 (MQ=255)
                                                                          aaCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 <  2:132453/112‑1 (MQ=255)
                                                                          aaCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGc                                                      >  1:1210460/1‑139 (MQ=255)
                                                                              ccGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCa                                                         >  2:114292/1‑132 (MQ=255)
                                                                              ccGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCa                                                         <  1:114292/132‑1 (MQ=255)
                                                                               cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 >  1:509343/1‑107 (MQ=255)
                                                                               cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 <  2:509343/107‑1 (MQ=255)
                                                                                 ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                               <  1:1632132/139‑1 (MQ=255)
                                                                                  cATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgc                                              <  1:1522176/139‑1 (MQ=255)
                                                                                  cATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgc                                              <  1:1481023/139‑1 (MQ=255)
                                                                                      ttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATgctgct                                          <  1:539234/139‑1 (MQ=255)
                                                                                           cGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCtggtgg                                     >  1:493188/1‑139 (MQ=255)
                                                                                             tGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                   <  2:1136677/139‑1 (MQ=255)
                                                                                                  cAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              <  2:267709/139‑1 (MQ=255)
                                                                                                  cAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              <  1:837424/139‑1 (MQ=255)
                                                                                                  cAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              >  2:1002119/1‑139 (MQ=255)
                                                                                                  cAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              <  1:277745/139‑1 (MQ=255)
                                                                                                            ccACGTTAGCCAGCCGATGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGaa                                                 >  1:43388/1‑110 (MQ=255)
                                                                                                            ccACGTTAGCCAGCCGATGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGaa                                                 <  2:43388/110‑1 (MQ=255)
                                                                                                                 ttAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                                              >  2:818713/1‑76 (MQ=255)
                                                                                                                 ttAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                                              <  1:818713/76‑1 (MQ=255)
                                                                                                                   agccagccGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTg             <  2:775029/139‑1 (MQ=255)
                                                                                                                         ccGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATgctgct                                          >  2:357931/1‑104 (MQ=255)
                                                                                                                         ccGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATgctgct                                          <  1:357931/104‑1 (MQ=255)
                                                                                                                         ccGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGTTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGa       >  2:1571275/1‑139 (MQ=255)
                                                                                                                         ccGACGCTTAGCGGATAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGa       >  1:214951/1‑139 (MQ=255)
                                                                                                                          cGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  1:1037693/139‑1 (MQ=255)
                                                                                                                          cGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  1:1554832/139‑1 (MQ=255)
                                                                                                                          cGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  1:462987/139‑1 (MQ=255)
                                                                                                                          cGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTACTGGAGCGGAc                                                                                 <  2:549914/64‑1 (MQ=255)
                                                                                                                          cGACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTACTGGAGCGGAc                                                                                 >  1:549914/1‑64 (MQ=255)
                                                                                                                          cGACGCTTAGCGGATAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  2:214951/139‑1 (MQ=255)
                                                                                                                           gACGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaaa     <  1:726908/139‑1 (MQ=255)
                                                                                                                            aCGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      <  1:214378/57‑1 (MQ=255)
                                                                                                                            aCGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      >  2:214378/1‑57 (MQ=255)
                                                                                                                            aCGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              >  2:385596/1‑113 (MQ=255)
                                                                                                                            aCGCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              <  1:385596/113‑1 (MQ=255)
                                                                                                                              gCTTAGCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTc  >  1:1318349/1‑139 (MQ=255)
                                                                                                                                   gCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaaa     <  1:172111/131‑1 (MQ=255)
                                                                                                                                   gCGGGTAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaaa     >  2:172111/1‑131 (MQ=255)
                                                                                                                                        |                                                                                                                                
CGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTC  >  NC_000913/4158456‑4158720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: