Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,213,680	C→T	*148* (TAG→TAA)	yjaB ←	GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,213,680	0	C	T	100.0%	312.0 / NA	96	*148* (TAG→TAA)	yjaB	GNAT‑family putative N‑acetyltransferase, acetyl coenzyme A‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/47);  total (49/47)

GCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGG  >  NC_000913/4213544‑4213813                                                                                                                                         |                                                                                                                                      gCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTa                                                                                                                                     <  2:859063/139‑1 (MQ=255)      ttCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                <  2:1638876/139‑1 (MQ=255)         cacaTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTa                                                                                                                                     >  2:1548865/1‑131 (MQ=255)         cacaTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTa                                                                                                                                     <  1:1548865/131‑1 (MQ=255)                aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                >  1:1083370/1‑129 (MQ=255)                aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                <  2:1083370/129‑1 (MQ=255)                 tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     >  2:1448465/1‑139 (MQ=255)                 tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     <  2:297303/139‑1 (MQ=255)                 tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGcc                                                                                                                     >  1:1656702/1‑139 (MQ=255)                        tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCa                                                                                                              >  2:1534401/1‑139 (MQ=255)                        tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCa                                                                                                              >  1:355130/1‑139 (MQ=255)                                      tGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       <  1:355112/116‑1 (MQ=255)                                      tGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       >  2:355112/1‑116 (MQ=255)                                         tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTc                                                                                             >  2:1633485/1‑139 (MQ=255)                                          aGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                            >  2:1058293/1‑139 (MQ=255)                                           gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTccc                                                                                           >  1:262628/1‑139 (MQ=255)                                           gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTccc                                                                                           >  1:1605502/1‑139 (MQ=255)                                            tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGa                                                                                                      <  2:177142/127‑1 (MQ=255)                                            tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGa                                                                                                      >  1:177142/1‑127 (MQ=255)                                              cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaa                                                                                        >  1:1381140/1‑139 (MQ=255)                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTa                                                                                                                                     >  1:500836/1‑92 (MQ=255)                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTa                                                                                                                                     <  2:500836/92‑1 (MQ=255)                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAAt                                                                                      >  1:266161/1‑139 (MQ=255)                                                    tGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  2:1605502/139‑1 (MQ=255)                                                      gCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCa                                                                                >  2:1097286/1‑139 (MQ=255)                                                           gCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGa                                                                                                                  <  2:1275350/100‑1 (MQ=255)                                                           gCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGa                                                                                                                  >  1:1275350/1‑100 (MQ=255)                                                              tAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                        <  1:1633485/139‑1 (MQ=255)                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  >  1:174903/1‑122 (MQ=255)                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  2:174903/122‑1 (MQ=255)                                                                       ataatCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTa                                                               <  2:262628/139‑1 (MQ=255)                                                                          atCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGAt                                                                                                     >  2:415900/1‑98 (MQ=255)                                                                          atCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGAt                                                                                                     <  1:415900/98‑1 (MQ=255)                                                                               gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaa                                                       >  2:40553/1‑139 (MQ=255)                                                                                  aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    <  1:1364698/139‑1 (MQ=255)                                                                                    cATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  1:1448465/139‑1 (MQ=255)                                                                                    cATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  1:1097286/139‑1 (MQ=255)                                                                                    cATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  2:554530/139‑1 (MQ=255)                                                                                     aTTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCAc                                                 <  1:1058293/139‑1 (MQ=255)                                                                                       tggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  2:227938/136‑1 (MQ=255)                                                                                       tggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  >  1:227938/1‑136 (MQ=255)                                                                                         gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    <  1:1103675/132‑1 (MQ=255)                                                                                         gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    >  2:1103675/1‑132 (MQ=255)                                                                                         gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttc                                             >  2:920827/1‑139 (MQ=255)                                                                                            ggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       <  1:1003143/62‑1 (MQ=255)                                                                                            ggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACg                                                                                                                       >  2:1003143/1‑62 (MQ=255)                                                                                            ggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTta                                          <  1:40553/139‑1 (MQ=255)                                                                                             gTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACg                                                                                                                                   >  1:697606/1‑49 (MQ=255)                                                                                             gTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACg                                                                                                                                   <  2:697606/49‑1 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                >  2:1513124/1‑51 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGccc                                                                                                                                <  1:1513124/51‑1 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGAtt                                                                                                                >  2:471486/1‑67 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGAtt                                                                                                                <  1:471486/67‑1 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  2:659249/129‑1 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  >  2:1242504/1‑129 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  1:1242504/129‑1 (MQ=255)                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  >  1:659249/1‑129 (MQ=255)                                                                                                 tAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGaa                                     >  1:396932/1‑139 (MQ=255)                                                                                                   aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  2:505950/92‑1 (MQ=255)                                                                                                   aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  >  1:505950/1‑92 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGcc                                                                                                                                 >  1:1465721/1‑40 (MQ=38)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGcc                                                                                                                                 <  2:1465721/40‑1 (MQ=38)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            >  2:981461/1‑61 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            <  1:981461/61‑1 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGt                                                                                   >  2:911001/1‑86 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGt                                                                                   <  1:911001/86‑1 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCAcc                                                <  2:1532134/121‑1 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCAcc                                                >  1:1532134/1‑121 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAcc                              >  1:330751/1‑139 (MQ=255)                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAAcc                              >  1:1548231/1‑139 (MQ=255)                                                                                                         aaGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCAcc                                                                              >  2:164116/1‑90 (MQ=255)                                                                                                         aaGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCAcc                                                                              <  1:164116/90‑1 (MQ=255)                                                                                                           gTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTCCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGcc                           >  1:13737/1‑139 (MQ=255)                                                                                                                gCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    >  1:169844/1‑109 (MQ=255)                                                                                                                gCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    <  2:169844/109‑1 (MQ=255)                                                                                                                 cccACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCa                     <  2:1656702/139‑1 (MQ=255)                                                                                                                   cACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            >  2:1145043/1‑50 (MQ=255)                                                                                                                   cACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            <  1:1145043/50‑1 (MQ=255)                                                                                                                     ccGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  1:467077/74‑1 (MQ=255)                                                                                                                     ccGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  >  2:467077/1‑74 (MQ=255)                                                                                                                     ccGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCAtttt                 <  1:297648/139‑1 (MQ=255)                                                                                                                      cGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg                <  2:1548231/139‑1 (MQ=255)                                                                                                                         gATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAACAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  1:255419/70‑1 (MQ=255)                                                                                                                         gATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAACAACGGATACGGTTTCCCCAAATCGTc                                                                                  >  2:255419/1‑70 (MQ=255)                                                                                                                           tGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  1:938983/100‑1 (MQ=255)                                                                                                                           tGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  >  2:938983/1‑100 (MQ=255)                                                                                                                           tGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCAt           >  2:1395288/1‑139 (MQ=255)                                                                                                                               aaaaaaTCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            >  1:1434905/1‑38 (MQ=255)                                                                                                                               aaaaaaTCGCTTACGCCCCCACATACGCCAGATTCAGc                                                                                                            <  2:1434905/38‑1 (MQ=255)                                                                                                                                aaaaaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACg      <  1:1534401/139‑1 (MQ=255)                                                                                                                                   aaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    <  1:715622/90‑1 (MQ=255)                                                                                                                                   aaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                    >  2:715622/1‑90 (MQ=255)                                                                                                                                   aaTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTCCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttg   <  2:13737/139‑1 (MQ=255)                                                                                                                                    aTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  >  1:737820/1‑59 (MQ=255)                                                                                                                                    aTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  2:737820/59‑1 (MQ=255)                                                                                                                                    aTCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACgttgg  >  1:559427/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      GCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGG  >  NC_000913/4213544‑4213813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑