Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,232,752 G→A A161V (GCG→GTG)  lysC ← lysine‑sensitive aspartokinase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,232,7520GA100.0% 207.0 / NA 63A161V (GCG→GTG) lysClysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/33);  total (30/33)

TGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTG  >  NC_000913/4232619‑4232885
                                                                                                                                     |                                                                                                                                     
tGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                                  <  1:656244/139‑1 (MQ=255)
                       ggCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                                           <  2:1507444/139‑1 (MQ=255)
                                      tACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACg                                                                                            <  1:682285/139‑1 (MQ=255)
                                        cGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGc                                                                                           >  2:1435424/1‑138 (MQ=255)
                                        cGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGc                                                                                           <  1:1435424/138‑1 (MQ=255)
                                        cGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                          <  1:1429055/139‑1 (MQ=255)
                                           ccTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACgg                                                                                                                                    >  2:1523980/1‑94 (MQ=255)
                                           ccTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACgg                                                                                                                                    <  1:1523980/94‑1 (MQ=255)
                                            cTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCAc                                                                                      <  1:1333962/139‑1 (MQ=255)
                                                aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                                     >  2:1247089/1‑136 (MQ=255)
                                                aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                                     <  1:1247089/136‑1 (MQ=255)
                                                  tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                                <  2:1004253/139‑1 (MQ=255)
                                                  tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                                <  2:738227/139‑1 (MQ=255)
                                                    tCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa                                                                              <  1:1186014/139‑1 (MQ=255)
                                                     cGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTg                                                                                                                     <  2:441035/99‑1 (MQ=255)
                                                     cGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTg                                                                                                                     >  1:441035/1‑99 (MQ=255)
                                                     cGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTAc                                                                             >  1:384680/1‑139 (MQ=255)
                                                                       gggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                                        <  2:491635/110‑1 (MQ=255)
                                                                       gggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                                        >  1:491635/1‑110 (MQ=255)
                                                                            aTCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCAGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACAt                                                      >  1:1048438/1‑139 (MQ=255)
                                                                             tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                                   <  1:1335836/125‑1 (MQ=255)
                                                                             tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                                   >  2:1335836/1‑125 (MQ=255)
                                                                             tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATc                                                     >  1:641959/1‑139 (MQ=255)
                                                                             tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATc                                                     >  2:1557437/1‑139 (MQ=255)
                                                                                       ttCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCgcgc                                           <  1:554606/139‑1 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                                  >  2:309995/1‑50 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                                  <  1:309995/50‑1 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGAc                                                                                                               >  2:934026/1‑69 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGAc                                                                                                               <  1:934026/69‑1 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                                      >  1:507116/1‑78 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                                      <  2:507116/78‑1 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAg                                         >  2:129161/1‑139 (MQ=255)
                                                                                         cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAg                                         >  2:1107/1‑139 (MQ=255)
                                                                                              agaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtct                                    <  1:263789/139‑1 (MQ=255)
                                                                                               gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGTCAGCACGACTATATCTGGCTCTGCACGACCATATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCat                                                                   <  1:1017293/107‑2 (MQ=255)
                                                                                               gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                                   <  1:927856/107‑1 (MQ=255)
                                                                                               gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                                   >  2:927856/1‑107 (MQ=255)
                                                                                               gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGACTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                                   >  2:1017293/1‑107 (MQ=255)
                                                                                                   tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                                     >  2:1446340/1‑85 (MQ=255)
                                                                                                   tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                                     <  1:1446340/85‑1 (MQ=255)
                                                                                                   tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaaca                               >  1:1186764/1‑139 (MQ=255)
                                                                                                    gggAGCTGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCa                                                                                                                   >  1:795385/1‑54 (MQ=255)
                                                                                                    gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCa                                                                                                                   <  2:795385/54‑1 (MQ=255)
                                                                                                    gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaacaa                              >  1:751568/1‑139 (MQ=255)
                                                                                                    gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaacaa                              >  1:1608997/1‑139 (MQ=255)
                                                                                                     ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACg                                                                                            <  1:690548/76‑1 (MQ=255)
                                                                                                     ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACg                                                                                            >  2:690548/1‑76 (MQ=255)
                                                                                                     ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCaacaaa                             <  1:1557437/139‑1 (MQ=255)
                                                                                                          agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAAcagca                        <  1:1380381/139‑1 (MQ=255)
                                                                                                               cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                          <  2:124193/68‑1 (MQ=255)
                                                                                                               cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                                          >  1:124193/1‑68 (MQ=255)
                                                                                                               cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa                                                                              >  1:610805/1‑80 (MQ=255)
                                                                                                               cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa                                                                              <  2:610805/80‑1 (MQ=255)
                                                                                                               cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTc                   <  2:1048438/139‑1 (MQ=255)
                                                                                                               cAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTc                   >  2:695098/1‑139 (MQ=255)
                                                                                                                   gcgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAAcagca                        <  2:1394700/130‑1 (MQ=255)
                                                                                                                   gcgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAAcagca                        >  1:1394700/1‑130 (MQ=255)
                                                                                                                   gcgGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACa               <  1:1150392/139‑1 (MQ=255)
                                                                                                                      gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACC‑AATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAATAAAcagca                        >  1:974179/1‑126 (MQ=255)
                                                                                                                      gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACC‑AATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAAcagca                        <  2:974179/126‑1 (MQ=255)
                                                                                                                         aGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                                >  2:103752/1‑68 (MQ=255)
                                                                                                                         aGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                                <  1:103752/68‑1 (MQ=255)
                                                                                                                                ccAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTg  >  2:1583339/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TGCCGTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTG  >  NC_000913/4232619‑4232885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑