Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,463	G→A	intergenic (+12/+3)	insO → / ← insI1	pseudogene, IS911 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/IS30 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,463	0	G	A	100.0%	208.3 / NA	66	intergenic (+12/+3)	insO/insI1	pseudogene, IS911 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/IS30 transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/36);  total (30/36)

ACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACA  >  NC_000913/4507331‑4507594                                                                                                                                     |                                                                                                                                    acTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                               <  1:860966/139‑1 (MQ=255)  cTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAAt                                                                                                                              >  1:1062471/1‑139 (MQ=255)       tgatgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTc                                                                                                                         >  2:844383/1‑139 (MQ=255)         atgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCaa                                                                                                                       <  2:1062471/139‑1 (MQ=255)             gCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCa                                                                                                                   >  1:1243351/1‑139 (MQ=255)              cGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATTGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCaa                                                                                                                  <  1:987395/139‑1 (MQ=255)                     gCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCt                                                                                                           <  2:1229644/139‑1 (MQ=255)                         aaCACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGt                                                                                                                          <  2:658709/120‑1 (MQ=255)                         aaCACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGt                                                                                                                          >  1:658709/1‑120 (MQ=255)                         aaCACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCa                                                                                                       >  1:1532907/1‑139 (MQ=255)                                      tctcCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                               <  2:732210/102‑1 (MQ=255)                                      tctcCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                               >  1:732210/1‑102 (MQ=255)                                        tcCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                              <  1:1517212/117‑1 (MQ=255)                                        tcCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                              >  2:1517212/1‑117 (MQ=255)                                        tcCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGgt                                                                                        >  2:522738/1‑139 (MQ=255)                                                    aGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTc                                                                            <  1:92019/139‑1 (MQ=255)                                                    aGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTc                                                                            >  1:1433166/1‑139 (MQ=255)                                                           aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAaca                                                                                                                <  2:337391/96‑1 (MQ=255)                                                           aTCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAaca                                                                                                                >  1:337391/1‑96 (MQ=255)                                                             cGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCa                                                                           >  1:1480528/1‑131 (MQ=255)                                                             cGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCa                                                                           <  2:1480528/131‑1 (MQ=255)                                                               aaaTACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                                 <  1:328372/139‑1 (MQ=255)                                                                   aCGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACGCCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGt                                                             <  1:844383/139‑1 (MQ=255)                                                                   aCGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGt                                                             <  2:1433166/139‑1 (MQ=255)                                                                    cGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCtt                                                                                                          <  2:1063771/93‑1 (MQ=255)                                                                    cGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCtt                                                                                                          >  1:1063771/1‑93 (MQ=255)                                                                    cGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                            <  2:1532907/139‑1 (MQ=255)                                                                         gCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                              <  1:171528/84‑1 (MQ=255)                                                                         gCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACAcc                                                                                                              >  2:171528/1‑84 (MQ=255)                                                                          cTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                                 <  1:535632/128‑1 (MQ=255)                                                                          cTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                                 >  2:535632/1‑128 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTGAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCttt                                                                                            <  2:1096214/98‑1 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCTATTATCTCttt                                                                                            >  1:1096214/1‑98 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                            >  1:434640/1‑130 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                            <  2:434640/130‑1 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTa                                                   >  1:560861/1‑139 (MQ=255)                                                                             aGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTa                                                   >  2:1446778/1‑139 (MQ=255)                                                                              ggAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAg                                                  <  1:251338/139‑1 (MQ=255)                                                                                aaaaaGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCt                                                <  2:1278065/139‑1 (MQ=255)                                                                                      cTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGagcag                                          <  1:522738/139‑1 (MQ=255)                                                                                      cTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGagcag                                          <  2:560861/139‑1 (MQ=255)                                                                                          aaCGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAc                                      <  1:1446778/139‑1 (MQ=255)                                                                                           aCGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAAcc                                     <  1:1065421/139‑1 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                               >  1:341184/1‑45 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCaa                                                                                                                               <  2:341184/45‑1 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                                 <  2:1597340/107‑1 (MQ=255)                                                                                               aTTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                                 >  1:1597340/1‑107 (MQ=255)                                                                                                        gagaATGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCAt                        <  2:137959/139‑1 (MQ=255)                                                                                                             tGAAATATTAACAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                            <  1:1096421/98‑1 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCg                                                               >  1:1058204/1‑95 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCg                                                               <  2:1058204/95‑1 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAgcagca                                        <  2:287834/118‑1 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAgcagca                                        >  1:287834/1‑118 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATg                       >  2:616428/1‑135 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATg                       <  1:616428/135‑1 (MQ=255)                                                                                                             tGAAATATTAAAAAGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAAGTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTc                                                            >  2:1096421/1‑98 (MQ=255)                                                                                                                       aaaaGGCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTggg         <  2:1354190/139‑1 (MQ=21)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAAtt                                                                                                    <  1:1656042/43‑1 (MQ=255)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAAtt                                                                                                    >  2:1656042/1‑43 (MQ=255)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTtct                                                                 <  1:1127954/78‑1 (MQ=255)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGa    >  2:1505497/1‑139 (MQ=17)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGa    >  1:1531470/1‑139 (MQ=17)                                                                                                                            gCTACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTGCAGTGTCTTtct                                                                 >  2:1127954/1‑78 (MQ=255)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACa  >  2:143526/1‑139 (MQ=14)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACa  <  2:468068/139‑1 (MQ=14)                                                                                                                              tACTGTAAATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACa  >  2:1406431/1‑139 (MQ=14)                                                                                                                                     |                                                                                                                                    ACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACA  >  NC_000913/4507331‑4507594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑