Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 375,307 C→T intergenic (+426/‑152) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,3070CT100.0% 84.9 / NA 29intergenic (+426/‑152)mhpE/mhpT4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

GCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTC  >  NC_000913/375171‑375441
                                                                                                                                        |                                                                                                                                      
gcgcGAGAATTTGTAGGCCTGATAAGACGCGTTAGCATCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAgtgt                                                                                                                                      <  2:712716/139‑1 (MQ=255)
     agaATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCAt                                                                                                                                 >  2:136160/1‑139 (MQ=37)
                gCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGc                                                                                                                      <  2:1096282/139‑1 (MQ=18)
                 ccTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGca                                                                                                                     >  1:188383/1‑139 (MQ=21)
                  cTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGcac                                                                                                                    >  2:31905/1‑139 (MQ=21)
                              gTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGca                                                                                                                     <  1:1352665/126‑1 (MQ=18)
                              gTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGca                                                                                                                     >  2:1352665/1‑126 (MQ=18)
                               ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCAt                                                                                                                          <  1:363076/120‑1 (MQ=18)
                               ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCAt                                                                                                                          >  2:363076/1‑120 (MQ=18)
                               ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGAt                                                                                                       <  2:1064245/139‑1 (MQ=25)
                                             ggCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAgtgt                                                                                                                                      >  2:1634912/1‑94 (MQ=21)
                                                                       ggcgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACGCGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                               <  1:1389236/139‑1 (MQ=255)
                                                                       ggcgTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                               <  1:16047/139‑1 (MQ=255)
                                                                                         ggCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                             >  2:1608181/1‑123 (MQ=255)
                                                                                         ggCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                                                             <  1:1608181/123‑1 (MQ=255)
                                                                                               cGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                          >  2:569138/1‑120 (MQ=255)
                                                                                               cGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                          <  1:569138/120‑1 (MQ=255)
                                                                                                  aTGGCGCGGGAATTTGTGGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCgg                                    <  2:542853/139‑1 (MQ=255)
                                                                                                   tGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGc                                   <  1:552062/139‑1 (MQ=255)
                                                                                                                tGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGcaca                                                                                                                   >  1:664744/1‑46 (MQ=255)
                                                                                                                tGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGcaca                                                                                                                   <  2:664744/46‑1 (MQ=255)
                                                                                                                tGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCa                      >  2:102164/1‑139 (MQ=255)
                                                                                                                         gATAAGACGCGTTAGTGTCGCATCAGGCTTCTGCACACGACTGCCGGATGCGATAAACGTGTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAg                                                  <  1:1016411/102‑1 (MQ=255)
                                                                                                                         gATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAg                                                  >  2:1016411/1‑102 (MQ=255)
                                                                                                                          aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCg                                                                        >  1:668277/1‑79 (MQ=255)
                                                                                                                          aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCg                                                                        <  2:668277/79‑1 (MQ=255)
                                                                                                                          aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                               <  2:1593568/88‑1 (MQ=255)
                                                                                                                          aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                                                               >  1:1593568/1‑88 (MQ=255)
                                                                                                                          aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                          <  2:1266255/93‑1 (MQ=255)
                                                                                                                          aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                                                          >  1:1266255/1‑93 (MQ=255)
                                                                                                                                    ttAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATGAGTCGCGATTCACCTGCACTc  >  1:975499/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTC  >  NC_000913/375171‑375441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: