Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,535,441 C→T pseudogene (439/1029 nt) yjhR → pseudogene, helicase family, putative frameshift suppressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,535,4410CT100.0% 284.5 / NA 87pseudogene (439/1029 nt)yjhRpseudogene, helicase family, putative frameshift suppressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/49);  total (38/49)

CTTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGTGTG  >  NC_000913/4535308‑4535572
                                                                                                                                     |                                                                                                                                   
ctTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACACGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCa                                                                                                                                <  1:1329955/139‑1 (MQ=255)
    tctcCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCAtttt                                                                                                                            <  2:638263/139‑1 (MQ=255)
    tctcCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCAtttt                                                                                                                            <  1:931295/139‑1 (MQ=255)
           tCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     <  1:186089/139‑1 (MQ=255)
           tCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     <  2:75145/139‑1 (MQ=255)
            cTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGa                                                                                                                    <  2:1446673/139‑1 (MQ=255)
            cTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGa                                                                                                                    <  2:1275479/139‑1 (MQ=255)
              aCTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAgg                                                                                                                  <  2:458247/139‑1 (MQ=255)
               cTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTcgccg                                                                                                                       <  1:873983/133‑1 (MQ=255)
               cTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTcgccg                                                                                                                       >  2:873983/1‑133 (MQ=255)
                tCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGt                                                                                                                >  1:533617/1‑139 (MQ=255)
                       aTGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTa                                                                                                              >  1:1561241/1‑134 (MQ=255)
                       aTGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTa                                                                                                              <  2:1561241/134‑1 (MQ=255)
                         gAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTAt                                                                                                             >  2:1178820/1‑133 (MQ=255)
                         gAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTAt                                                                                                             <  1:1178820/133‑1 (MQ=255)
                              tGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     <  1:1331878/120‑1 (MQ=255)
                              tGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     >  2:1331878/1‑120 (MQ=255)
                                 cAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATAtctc                                                                                               >  2:1381034/1‑139 (MQ=255)
                                       tCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttct                                                                                         <  1:425848/139‑1 (MQ=255)
                                         ttGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttcttc                                                                                       <  1:1187083/139‑1 (MQ=255)
                                          tGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGc                                                                                                         <  1:658542/120‑1 (MQ=255)
                                          tGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGc                                                                                                         >  2:658542/1‑120 (MQ=255)
                                          tGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                      <  1:1544389/139‑1 (MQ=255)
                                            aTAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGa                                                                                    <  1:137823/139‑1 (MQ=255)
                                              aGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGata                                                                                  >  1:890877/1‑139 (MQ=255)
                                                 aaCAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGataata                                                                               <  2:639803/139‑1 (MQ=255)
                                                   cAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGAATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCCCTGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATaattat                                                                             >  2:942456/1‑139 (MQ=255)
                                                           tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     >  2:1629971/1‑91 (MQ=255)
                                                           tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     <  1:1629971/91‑1 (MQ=255)
                                                                aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGt                                                                         <  2:932125/130‑1 (MQ=255)
                                                                aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGt                                                                         >  1:932125/1‑130 (MQ=255)
                                                                     tGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGttt                                                           >  1:742913/1‑139 (MQ=255)
                                                                      gCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCCTTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                      <  2:1111301/111‑1 (MQ=255)
                                                                      gCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttct                                                                                         <  1:5826/108‑1 (MQ=255)
                                                                      gCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttct                                                                                         >  2:5826/1‑108 (MQ=255)
                                                                      gCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                      >  1:1111301/1‑111 (MQ=255)
                                                                       cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     >  2:324106/1‑79 (MQ=255)
                                                                       cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     <  1:324106/79‑1 (MQ=255)
                                                                       cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCa                                                                                                        <  2:522253/92‑1 (MQ=255)
                                                                       cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCa                                                                                                        >  1:522253/1‑92 (MQ=255)
                                                                       cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCa                                                         >  2:386965/1‑139 (MQ=255)
                                                                          tctcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGaa                                                      >  1:277707/1‑139 (MQ=255)
                                                                            tcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTcgccgc                                                                                                                      >  1:1441671/1‑73 (MQ=255)
                                                                            tcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTcgccgc                                                                                                                      <  2:1441671/73‑1 (MQ=255)
                                                                             cACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGa                                                                                                                    >  1:565721/1‑74 (MQ=255)
                                                                             cACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGa                                                                                                                    <  2:565721/74‑1 (MQ=255)
                                                                              aCGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                  <  2:277707/139‑1 (MQ=255)
                                                                              aCGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                  <  1:1368428/139‑1 (MQ=255)
                                                                               cgcgCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGAc                                                 <  1:156136/139‑1 (MQ=255)
                                                                               cgcgCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGAc                                                 >  2:588948/1‑139 (MQ=255)
                                                                                                tCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCAtttt                                                                                                                            >  2:912864/1‑47 (MQ=255)
                                                                                                tCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCAtttt                                                                                                                            <  1:912864/47‑1 (MQ=255)
                                                                                                tCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGca                                >  1:183018/1‑139 (MQ=255)
                                                                                                  tGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGa                                                       <  2:1582674/114‑1 (MQ=255)
                                                                                                  tGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGa                                                       >  1:1582674/1‑114 (MQ=255)
                                                                                                  tGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGcaca                              >  1:200278/1‑139 (MQ=255)
                                                                                                   ggTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttcttc                                                                                       <  1:477346/81‑1 (MQ=255)
                                                                                                   ggTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttcttc                                                                                       >  2:477346/1‑81 (MQ=255)
                                                                                                   ggTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACAt                             <  1:559133/139‑1 (MQ=255)
                                                                                                    gTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGa                                                                                                                    >  1:561474/1‑51 (MQ=255)
                                                                                                    gTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGa                                                                                                                    <  2:561474/51‑1 (MQ=255)
                                                                                                       tttGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     <  2:104022/47‑1 (MQ=255)
                                                                                                       tttGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCcgcg                                                                                                                     >  1:104022/1‑47 (MQ=255)
                                                                                                       tttGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATctct                                                                                              <  2:815226/70‑1 (MQ=255)
                                                                                                       tttGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATctct                                                                                              >  1:815226/1‑70 (MQ=255)
                                                                                                         tGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                  >  1:1449245/1‑112 (MQ=255)
                                                                                                         tGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                  <  2:1449245/112‑1 (MQ=255)
                                                                                                              atatGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                      >  1:422062/1‑71 (MQ=255)
                                                                                                              atatGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                      <  2:422062/71‑1 (MQ=255)
                                                                                                              atatGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTAAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTc                  >  2:1041946/1‑139 (MQ=255)
                                                                                                                 tGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAAc               >  2:715984/1‑139 (MQ=255)
                                                                                                                  ggACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACg              <  2:742913/139‑1 (MQ=255)
                                                                                                                       ttATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTTGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACTTACACAAATTTCAACGCTGCa         <  1:332287/139‑1 (MQ=255)
                                                                                                                       ttATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa         <  2:183018/139‑1 (MQ=255)
                                                                                                                       ttATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa         <  1:588948/139‑1 (MQ=255)
                                                                                                                       ttATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa         <  2:890877/139‑1 (MQ=255)
                                                                                                                        tATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTAtt                                                                                                            <  1:712489/39‑1 (MQ=255)
                                                                                                                        tATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTAtt                                                                                                            >  2:712489/1‑39 (MQ=255)
                                                                                                                        tATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttct                                                                                         <  1:652781/58‑1 (MQ=255)
                                                                                                                        tATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttct                                                                                         >  2:652781/1‑58 (MQ=255)
                                                                                                                         aTCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                  >  2:1222056/1‑96 (MQ=255)
                                                                                                                         aTCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                  <  1:1222056/96‑1 (MQ=255)
                                                                                                                             agaTGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGAc                                                 <  1:525356/93‑1 (MQ=255)
                                                                                                                             agaTGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGAc                                                 >  2:525356/1‑93 (MQ=255)
                                                                                                                              gaTGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGtgtg  <  2:200278/139‑1 (MQ=255)
                                                                                                                                tGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCtttt                                                                                           >  1:1278960/1‑48 (MQ=255)
                                                                                                                                tGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCtttt                                                                                           <  2:1278960/48‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CTTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGTGTG  >  NC_000913/4535308‑4535572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: