Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,477	A→G	H355R (CAC→CGC)	prfC →	peptide chain release factor RF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,477	0	A	G	100.0%	136.2 / NA	41	H355R (CAC→CGC)	prfC	peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/20);  total (21/20)

CCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCCGT  >  NC_000913/4610346‑4610610                                                                                                                                    |                                                                                                                                      ccgcgaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTgaa                                                                                                                                <  1:209313/139‑1 (MQ=255)          cgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGt                                                                                                                         >  2:207312/1‑137 (MQ=255)          cgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGt                                                                                                                         <  1:207312/137‑1 (MQ=255)                           tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTgaa                                                                                                                                <  2:236551/113‑1 (MQ=255)                           tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTgaa                                                                                                                                >  1:236551/1‑113 (MQ=255)                                tCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGc                                                                                                 >  2:1118783/1‑139 (MQ=255)                                  cGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcac                                                                                               <  2:1453061/139‑1 (MQ=255)                                   ggTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGAGCGTTCGCGCGGTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcaca                                                                                              >  2:674017/1‑139 (MQ=255)                                     tAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTACTACGCGCTGACCTTTATGGCGGATGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAAc                                                                                            <  1:1031542/139‑1 (MQ=255)                                      aaaTATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAAcc                                                                                           >  1:866464/1‑139 (MQ=255)                                        atatGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCAc                                                                                         >  1:87115/1‑139 (MQ=255)                                          atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGc                                                                                                     >  1:1412356/1‑125 (MQ=255)                                          atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGc                                                                                                     <  2:1412356/125‑1 (MQ=255)                                          atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACgg                                                                                       >  1:910355/1‑139 (MQ=255)                                            gAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                                     >  1:311944/1‑139 (MQ=255)                                            gAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                                     <  2:366620/139‑1 (MQ=255)                                            gAAAAAGGCATCAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                                     <  1:1072845/139‑1 (MQ=255)                                                            tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:910355/139‑1 (MQ=255)                                                            tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:87115/139‑1 (MQ=255)                                                            tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:404564/139‑1 (MQ=255)                                                                    gTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     >  1:674226/1‑131 (MQ=255)                                                                    gTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                                     <  2:674226/131‑1 (MQ=255)                                                                             gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                >  1:737217/1‑127 (MQ=255)                                                                             gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                <  2:737217/127‑1 (MQ=255)                                                                             gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTg                                                    >  1:1182786/1‑139 (MQ=255)                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                                          >  2:958592/1‑100 (MQ=255)                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                                          <  1:958592/100‑1 (MQ=255)                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTga                                                   <  2:866464/139‑1 (MQ=255)                                                                              cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTga                                                   >  2:1256947/1‑139 (MQ=255)                                                                                    aTGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAgatgat                                             <  1:1256947/139‑1 (MQ=255)                                                                                      gtggtgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCCGCGACACCTTTACCCAGGGTGAGAtgatga                                           >  1:319427/1‑139 (MQ=255)                                                                                        ggtgATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAg                                         >  1:847771/1‑139 (MQ=255)                                                                                               tcCGACGCGCTGACCTTTATGGCGGGTGACCGTTcgcgcg                                                                                                                                     >  1:758146/1‑40 (MQ=255)                                                                                                      cgcTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                >  2:393723/1‑102 (MQ=255)                                                                                                      cgcTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt                                                                <  1:393723/102‑1 (MQ=255)                                                                                                        cTGACCTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGa                         >  1:81061/1‑139 (MQ=255)                                                                                                            ccTTTATGGCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt                     <  2:311944/139‑1 (MQ=255)                                                                                                                    gCGGGTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAg             <  1:1118783/139‑1 (MQ=255)                                                                                                                       ggTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt                     >  2:413739/1‑128 (MQ=255)                                                                                                                       ggTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt                     <  1:413739/128‑1 (MQ=255)                                                                                                                       ggTGACCGTTCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAAc          >  1:1604833/1‑139 (MQ=255)                                                                                                                               ttCGCGCGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCcgt  >  1:98578/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      CCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCACCAGAACTGTTCCGT  >  NC_000913/4610346‑4610610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑