Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,510	A→G	E43G (GAG→GGG)	ybaO →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,510	0	A	G	100.0%	161.0 / NA	45	E43G (GAG→GGG)	ybaO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

AATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCA  >  NC_000913/468394‑468635                                                                                                                     |                                                                                                                              aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCCTATCGGc                                                                                                         >  2:992235/1‑139 (MQ=255)      aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCATTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGt                                                                                                    >  1:1450169/1‑139 (MQ=255)         gTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGc                                                                                                 <  1:1649251/139‑1 (MQ=255)                 ctgGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                         >  2:194078/1‑107 (MQ=255)                 ctgGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                         <  1:194078/107‑1 (MQ=255)                              agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAAt                                                                            >  2:62524/1‑139 (MQ=255)                                       gCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCgg                                                                   >  1:1372815/1‑139 (MQ=255)                                        cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                         <  1:731458/84‑1 (MQ=255)                                        cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                         >  2:731458/1‑84 (MQ=255)                                             tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc                                                                       <  1:4054/129‑1 (MQ=255)                                             tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc                                                                       >  2:4054/1‑129 (MQ=255)                                             tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                             >  2:563597/1‑139 (MQ=255)                                              ctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg                                                                                                                      <  1:1619679/81‑1 (MQ=255)                                              ctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg                                                                                                                      >  2:1619679/1‑81 (MQ=255)                                                   tGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCttt                                                       >  1:1464287/1‑139 (MQ=255)                                                         cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt                                                 >  2:973536/1‑139 (MQ=255)                                                           cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                             <  2:358920/125‑1 (MQ=255)                                                           cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                             >  1:358920/1‑125 (MQ=255)                                                           cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                             <  1:1212556/125‑1 (MQ=255)                                                           cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                             >  2:1212556/1‑125 (MQ=255)                                                                 gAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaaaaa                                         <  2:1464287/139‑1 (MQ=255)                                                                  aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAAc                                        >  1:1079734/1‑139 (MQ=255)                                                                   aGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACg                                       <  1:62524/139‑1 (MQ=255)                                                                   aGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACg                                       <  2:1450169/139‑1 (MQ=255)                                                                        ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAg                                                                                                     <  1:1238514/72‑1 (MQ=255)                                                                        ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAg                                                                                                     >  2:1238514/1‑72 (MQ=255)                                                                          aaTCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATc                                <  2:1079734/139‑1 (MQ=255)                                                                                        cccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCa                                                                                                        >  1:10621/1‑53 (MQ=255)                                                                                        cccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCa                                                                                                        <  2:10621/53‑1 (MQ=255)                                                                                          cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTa                >  2:1407439/1‑139 (MQ=255)                                                                                          cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTa                >  2:1464054/1‑139 (MQ=255)                                                                                          cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTa                >  2:1361036/1‑139 (MQ=255)                                                                                              cTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACAt                                 <  2:217050/118‑1 (MQ=255)                                                                                              cTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACAt                                 >  1:217050/1‑118 (MQ=255)                                                                                                ggAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg          <  1:1345986/139‑1 (MQ=255)                                                                                                ggAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCCTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg          <  1:992235/139‑1 (MQ=255)                                                                                                  aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                                       <  1:219789/60‑1 (MQ=255)                                                                                                  aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                                       >  2:219789/1‑60 (MQ=255)                                                                                                       ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGa                                                                                  >  1:120702/1‑60 (MQ=255)                                                                                                       ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGa                                                                                  <  2:120702/60‑1 (MQ=255)                                                                                                       ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTc   >  2:876405/1‑139 (MQ=255)                                                                                                        cTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATcc                                                                                     <  1:40510/56‑1 (MQ=255)                                                                                                        cTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATcc                                                                                     >  2:40510/1‑56 (MQ=255)                                                                                                        cTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCa  <  1:1464054/139‑1 (MQ=255)                                                                                                        cTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCa  <  1:1407439/139‑1 (MQ=255)                                                                                                                     |                                                                                                                              AATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCA  >  NC_000913/468394‑468635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑