Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	478,533	C→T	A29T (GCC→ACC)	ylaB ←	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	478,533	0	C	T	100.0%	319.9 / NA	98	A29T (GCC→ACC)	ylaB	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/52);  total (46/52)

CAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGG  >  NC_000913/478397‑478665                                                                                                                                         |                                                                                                                                     cAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtca                                                                                                                                    >  2:1532960/1‑139 (MQ=255)  aGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcag                                                                                                                                   >  2:407177/1‑139 (MQ=255)                     ttCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                                <  1:337951/139‑1 (MQ=255)                      tCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAg                                                                                                               >  1:506345/1‑139 (MQ=255)                              cTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAAt                                                                                                       >  1:1315424/1‑139 (MQ=255)                                gCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATAc                                                                                                     >  2:764049/1‑139 (MQ=255)                                   gcgcATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGGCAGGCAGCAATACTGa                                                                                                  >  2:334456/1‑139 (MQ=255)                                            tCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAg                                                                                         <  1:607557/139‑1 (MQ=255)                                              ccTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                           >  2:744819/1‑119 (MQ=255)                                              ccTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                           <  1:744819/119‑1 (MQ=255)                                              ccTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                       <  2:1524335/139‑1 (MQ=255)                                                 tgtgTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTc                                                                                    >  2:191502/1‑139 (MQ=255)                                                 tgtgTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAACTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTc                                                                                    >  1:1117530/1‑139 (MQ=255)                                                   tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                              >  1:484773/1‑111 (MQ=255)                                                   tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                              <  2:484773/111‑1 (MQ=255)                                                       gCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGaaa                                                                              >  1:1607661/1‑139 (MQ=255)                                                        ccACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAAt                                                                             >  1:906683/1‑139 (MQ=255)                                                        ccACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAAt                                                                             >  1:951144/1‑139 (MQ=255)                                                            cTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAgg                                                                         <  2:1475158/139‑1 (MQ=255)                                                             ttATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGc                                                                        <  2:1117530/139‑1 (MQ=255)                                                               aTTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCg                                                                      >  1:2887/1‑139 (MQ=255)                                                                 tGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGAc                                                                    <  2:506345/139‑1 (MQ=255)                                                                    cTCGAATAGCGACGCGGGAGGAATATGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                                 <  1:764049/139‑1 (MQ=255)                                                                      cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATg                                                                                                                            <  1:981303/78‑1 (MQ=255)                                                                      cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                   <  1:241718/119‑1 (MQ=255)                                                                      cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                   >  2:241718/1‑119 (MQ=255)                                                                      cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGAt                                                               >  1:645517/1‑139 (MQ=255)                                                                      cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGAt                                                               >  2:762392/1‑139 (MQ=255)                                                                      cGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGAAGTTTCTACCTGCTGATGGGTCAGCCAGATg                                                                                                                            >  2:981303/1‑78 (MQ=255)                                                                        aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGt                                                             >  1:308609/1‑139 (MQ=255)                                                                        aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGt                                                             >  2:482103/1‑139 (MQ=255)                                                                                  cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                           >  2:1195089/1‑83 (MQ=255)                                                                                  cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                           <  1:1195089/83‑1 (MQ=255)                                                                                  cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                   <  2:2887/139‑1 (MQ=255)                                                                                   gggAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                                >  2:934775/1‑77 (MQ=255)                                                                                   gggAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                                <  1:934775/77‑1 (MQ=255)                                                                                            aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAGATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         >  1:580630/1‑139 (MQ=255)                                                                                            aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAGATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         <  2:580630/139‑1 (MQ=255)                                                                                            aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         <  2:1315424/139‑1 (MQ=255)                                                                                            aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         <  2:35197/139‑1 (MQ=255)                                                                                            aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         <  2:1607661/139‑1 (MQ=255)                                                                                            aGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGATCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         <  1:131641/139‑1 (MQ=255)                                                                                              gTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGc                                                                                                                     <  2:1186359/61‑1 (MQ=255)                                                                                              gTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGc                                                                                                                     >  1:1186359/1‑61 (MQ=255)                                                                                              gTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCt                                       >  1:988350/1‑139 (MQ=255)                                                                                               tATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                                <  1:43266/65‑1 (MQ=255)                                                                                               tATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACa                                                                                                                >  2:43266/1‑65 (MQ=255)                                                                                                   cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtg                                                         <  1:636454/116‑1 (MQ=255)                                                                                                   cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCgtg                                                         >  2:636454/1‑116 (MQ=255)                                                                                                   cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttatta                                  >  1:133311/1‑139 (MQ=255)                                                                                                   cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttatta                                  >  2:1374964/1‑139 (MQ=255)                                                                                                   cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttatta                                  >  2:1079036/1‑139 (MQ=255)                                                                                                     gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                                <  2:517322/139‑1 (MQ=255)                                                                                                     gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAac                                <  2:645517/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTATGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:1050600/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                              <  2:105143/60‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                              >  1:105143/1‑60 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATGCTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  2:951144/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGc                                                                        >  1:1168059/1‑98 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGc                                                                        <  2:1168059/98‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         <  2:664326/129‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                         >  1:664326/1‑129 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:482103/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  2:252914/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:762392/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:214532/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:1079036/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:1532960/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  1:1379378/139‑1 (MQ=255)                                                                                                      ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                               <  2:988350/139‑1 (MQ=255)                                                                                                       tctTCAATAAACGATGTTTCTGCCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTTACAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                              <  2:965405/139‑1 (MQ=255)                                                                                                              tAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                       >  1:1626309/1‑107 (MQ=255)                                                                                                              tAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                       <  2:1626309/107‑1 (MQ=255)                                                                                                               aaaCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGGCAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAgg                      <  1:334456/139‑1 (MQ=255)                                                                                                                aaCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                       >  1:1194316/1‑73 (MQ=255)                                                                                                                aaCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                       <  2:1194316/73‑1 (MQ=255)                                                                                                                   gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                    >  2:881638/1‑121 (MQ=255)                                                                                                                   gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttat                                    <  1:881638/121‑1 (MQ=255)                                                                                                                   gATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGaaa                  >  2:1117914/1‑139 (MQ=255)                                                                                                                          cTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                                 <  2:1533708/85‑1 (MQ=255)                                                                                                                          cTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                                 >  1:1533708/1‑85 (MQ=255)                                                                                                                               tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                           >  1:526799/1‑38 (MQ=255)                                                                                                                               tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                           <  2:526799/38‑1 (MQ=255)                                                                                                                                gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                   <  2:131815/61‑1 (MQ=255)                                                                                                                                gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                   >  1:131815/1‑61 (MQ=255)                                                                                                                                gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                              >  1:494814/1‑114 (MQ=255)                                                                                                                                gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAacac                              <  2:494814/114‑1 (MQ=255)                                                                                                                                 ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                      <  1:491948/41‑1 (MQ=255)                                                                                                                                 ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                      >  2:491948/1‑41 (MQ=255)                                                                                                                                 ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                   <  2:464467/92‑1 (MQ=255)                                                                                                                                 ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                   >  1:464467/1‑92 (MQ=255)                                                                                                                                   gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGAc                                                                    >  1:1135520/1‑73 (MQ=255)                                                                                                                                   gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGAc                                                                    <  2:1135520/73‑1 (MQ=255)                                                                                                                                   gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTgg  >  2:119895/1‑139 (MQ=255)                                                                                                                                    aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGaaa                  >  1:315680/1‑122 (MQ=255)                                                                                                                                    aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGaaa                  <  2:315680/122‑1 (MQ=255)                                                                                                                                     tGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                    <  2:1019456/119‑1 (MQ=255)                                                                                                                                     tGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                    >  1:1019456/1‑119 (MQ=255)                                                                                                                                      gggTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGc                                              <  1:994637/92‑1 (MQ=255)                                                                                                                                      gggTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGc                                              >  2:994637/1‑92 (MQ=255)                                                                                                                                         |                                                                                                                                     CAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGG  >  NC_000913/478397‑478665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑