Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	486,641	T→C	S36P (TCA→CCA)	mscK →	mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	486,641	0	T	C	100.0%	296.7 / NA	84	S36P (TCA→CCA)	mscK	mechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/43);  total (41/43)

CGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACC  >  NC_000913/486505‑486766                                                                                                                                         |                                                                                                                                 cGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCa                                                                                                                                <  2:429675/139‑1 (MQ=255)   ttcttcAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATc                                                                                                                              <  2:442384/139‑1 (MQ=255)         aGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑Tgg                                                                                                                     >  1:1183314/1‑139 (MQ=255)             tCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGAt                                                                                                                 >  1:1073075/1‑139 (MQ=255)                gAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTg                                                                                                              >  1:760505/1‑139 (MQ=255)                          ttcatCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAg                                                                                                    <  2:1466924/139‑1 (MQ=255)                               catGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGAc                                                                                               >  1:407516/1‑139 (MQ=255)                               catGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGAc                                                                                               >  2:888839/1‑139 (MQ=255)                                  gACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTg                                                                                            >  1:831100/1‑139 (MQ=255)                                      aTGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAgg                                                                                        <  1:1595538/139‑1 (MQ=255)                                      aTGTTCCAGTATTACAAACGATCACGGCATTCTGTTTTTTCAGCATTTATTGCTTTTGTTTTAGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAgg                                                                                        <  2:294625/139‑1 (MQ=255)                                       tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCAt                                                                                                                               >  2:767521/1‑102 (MQ=255)                                       tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCAt                                                                                                                               <  1:767521/102‑1 (MQ=255)                                       tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTg                                                                                            >  2:917286/1‑134 (MQ=255)                                       tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTg                                                                                            <  1:917286/134‑1 (MQ=255)                                        gTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                      <  2:831100/139‑1 (MQ=255)                                        gTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                      >  1:922151/1‑139 (MQ=255)                                          tCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAATAGCGGACCTGCAGGCGCa                                                                                    <  2:922151/139‑1 (MQ=255)                                              gTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACtt                                                                                >  1:1127450/1‑139 (MQ=255)                                               tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                               >  1:701835/1‑139 (MQ=255)                                               tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                               <  2:407516/139‑1 (MQ=255)                                                 ttACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGAc                                                                             >  1:397085/1‑139 (MQ=255)                                                 ttACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGAc                                                                             >  1:150914/1‑139 (MQ=255)                                                   aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                           <  2:1127450/139‑1 (MQ=255)                                                   aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGAGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                           <  1:197543/139‑1 (MQ=255)                                                   aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAAAACGGCGTTTGAGCGGGCGCCATCGAA‑‑‑TGGAGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                           >  2:197543/1‑139 (MQ=255)                                                      aaCGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACt                                                                        >  2:612341/1‑139 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTGTTGCTTTTGTTTTTGACTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTg                                                                                                                   >  2:955976/1‑93 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTg                                                                                                                   <  1:955976/93‑1 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGAt                                                                                                                 <  2:270740/95‑1 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGAt                                                                                                                 >  1:270740/1‑95 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGCCAAAAGCGGAGCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  2:1053822/1‑139 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                               <  2:1055930/129‑1 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                               >  1:1055930/1‑129 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  1:30060/1‑139 (MQ=255)                                                         gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaa                                                                     >  1:802959/1‑139 (MQ=255)                                                           tCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                   >  2:207195/1‑139 (MQ=255)                                                           tCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaata                                                                   <  1:485401/139‑1 (MQ=255)                                                            cACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACtaaataa                                                                  >  2:1467342/1‑139 (MQ=255)                                                                 cATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaaa                                                             >  2:1408502/1‑139 (MQ=255)                                                                   ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGa                                                                                                                            <  1:901630/77‑1 (MQ=255)                                                                   ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGa                                                                                                                            >  2:901630/1‑77 (MQ=255)                                                                   ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaaaaa                                                           <  1:612341/139‑1 (MQ=255)                                                                     ttGTTTTTTCAGCACTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGa                                                         >  2:1516488/1‑139 (MQ=255)                                                                             tCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTg                                                 >  1:547772/1‑139 (MQ=255)                                                                               aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  2:802959/139‑1 (MQ=255)                                                                               aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  1:1516488/139‑1 (MQ=255)                                                                               aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  1:1408502/139‑1 (MQ=255)                                                                               aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  2:30060/139‑1 (MQ=255)                                                                                    ttATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGa                                          <  2:397085/139‑1 (MQ=255)                                                                                    ttATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TAGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGa                                          <  2:1183314/139‑1 (MQ=255)                                                                                         gCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAAc                                     <  1:888839/139‑1 (MQ=255)                                                                                           ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCa                                                                                                                                <  2:1210351/49‑1 (MQ=255)                                                                                           ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCa                                                                                                                                >  1:1210351/1‑49 (MQ=255)                                                                                            tttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACta                                                                       <  1:715719/102‑1 (MQ=255)                                                                                            tttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACta                                                                       >  2:715719/1‑102 (MQ=255)                                                                                             ttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAC‑‑‑TGGTGATCTGCCGACAAAAGCGGCCCTGCAGGCGCAACTGGACTCACTAACTAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGt                                 <  1:1053822/139‑1 (MQ=255)                                                                                             ttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGt                                 <  2:760505/139‑1 (MQ=255)                                                                                             ttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCAGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGt                                 <  2:1125839/139‑1 (MQ=255)                                                                                              tgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGaa                                                                                                                           <  1:707900/51‑1 (MQ=255)                                                                                              tgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGaa                                                                                                                           >  2:707900/1‑51 (MQ=255)                                                                                              tgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACaaaa                                                                                                     >  1:371474/1‑70 (MQ=255)                                                                                              tgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACaaaa                                                                                                     <  2:371474/70‑1 (MQ=255)                                                                                               gtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgc                               <  1:1578704/139‑1 (MQ=255)                                                                                                 ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAGAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                             <  2:150914/139‑1 (MQ=255)                                                                                                    tgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                    >  1:1491324/1‑113 (MQ=255)                                                                                                    tgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCttt                                                    <  2:1491324/113‑1 (MQ=255)                                                                                                    tgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGcagcag                          >  2:644079/1‑139 (MQ=255)                                                                                                     gtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAgg                         <  1:1467342/139‑1 (MQ=255)                                                                                                          gTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGa                                                                              >  2:582650/1‑81 (MQ=255)                                                                                                          gTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGa                                                                              <  1:582650/81‑1 (MQ=255)                                                                                                                ccAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                             >  1:209602/1‑124 (MQ=255)                                                                                                                ccAGAACACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                             <  2:209602/124‑1 (MQ=255)                                                                                                                 cAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                <  2:37514/139‑1 (MQ=255)                                                                                                                    aaCACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               <  2:1356831/102‑1 (MQ=255)                                                                                                                    aaCACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                               >  1:1356831/1‑102 (MQ=255)                                                                                                                    aaCACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACAt          >  1:305314/1‑139 (MQ=255)                                                                                                                    aaCACGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACAt          >  2:565059/1‑139 (MQ=255)                                                                                                                     acacGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACAtt         >  1:686671/1‑139 (MQ=255)                                                                                                                        cGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGAc                                                                             >  2:497185/1‑68 (MQ=255)                                                                                                                        cGGCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGAc                                                                             <  1:497185/68‑1 (MQ=255)                                                                                                                         ggCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                >  1:957861/1‑128 (MQ=255)                                                                                                                         ggCGTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAAGAAACAAGAAGATCTTTCTGCTCTGGACAAACTGGTGCAGCAGGATCTGACAg                <  2:957861/128‑1 (MQ=255)                                                                                                                            gTTTGCGCGGGCGCCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCAcc  <  1:653361/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 CGTTCTTCAGGTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAA‑‑‑TGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACC  >  NC_000913/486505‑486766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑