Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,216,996	Δ2 bp		[ymgC]	[ymgC]

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,216,995	0	A	.	100.0%	234.9 / NA	57	coding (248/249 nt)	ymgC	Blue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/31);  total (26/31)
*	NC_000913	1,216,996	0	G	.	100.0%	234.9 / NA	57	coding (249/249 nt)	ymgC	Blue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (26/31);  total (26/31)

GCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NC_000913/1216859‑1217125                                                                                                                                         ||                                                                                                                                  gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  >  2:745529/1‑137 (MQ=255) gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  >  2:437771/1‑137 (MQ=255) gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  <  2:19623/139‑3 (MQ=255)        gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  2:374297/139‑1 (MQ=255)        gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  2:209168/139‑1 (MQ=255)        gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  2:1125738/139‑1 (MQ=255)                          aaaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                       >  1:1181937/1‑139 (MQ=255)                           aaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:437771/139‑1 (MQ=255)                             ccAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:745529/139‑1 (MQ=255)                                           tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGc                                                                                      >  2:358056/1‑139 (MQ=255)                                               aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  >  2:438369/1‑91 (MQ=255)                                               aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  <  1:438369/93‑3 (MQ=255)                                                    tATCTGCAAATATTGCAGTACATTGAAAGGCATAGGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             >  2:975131/1‑139 (MQ=255)                                                         gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:779267/139‑1 (MQ=255)                                                            aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                          >  2:611901/1‑134 (MQ=255)                                                            aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                          <  1:611901/134‑1 (MQ=255)                                                                ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:1023593/104‑1 (MQ=255)                                                                ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    >  2:1023593/1‑104 (MQ=255)                                                                    aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      >  1:502094/1‑98 (MQ=255)                                                                    aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  2:502094/98‑1 (MQ=255)                                                                         aTTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:341682/139‑1 (MQ=255)                                                                           tGAAATGCATACGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATTCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  1:975131/139‑1 (MQ=255)                                                                           tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:20903/137‑1 (MQ=255)                                                                           tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        >  2:20903/1‑137 (MQ=255)                                                                                tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  1:767162/1‑116 (MQ=255)                                                                                tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  2:767162/116‑1 (MQ=255)                                                                                  cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                       <  1:1202050/83‑1 (MQ=255)                                                                                  cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                       >  2:1202050/1‑83 (MQ=255)                                                                                  cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             >  2:59039/1‑109 (MQ=255)                                                                                  cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             <  1:59039/109‑1 (MQ=255)                                                                                         cTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  2:1181937/139‑1 (MQ=255)                                                                                            cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGTGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCa                                     >  2:1415986/1‑139 (MQ=255)                                                                                                aaCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 <  1:358056/139‑1 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  1:974590/50‑1 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         >  2:974590/1‑50 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      >  2:644061/1‑69 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:644061/69‑1 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:264239/99‑1 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  2:264239/1‑99 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      >  1:360833/1‑117 (MQ=255)                                                                                                 aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:360833/117‑1 (MQ=255)                                                                                                              ttaATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                              >  1:646779/1‑112 (MQ=255)                                                                                                              ttaATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                              <  2:646779/112‑1 (MQ=255)                                                                                                               taATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:948656/85‑1 (MQ=255)                                                                                                               taATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  2:948656/1‑85 (MQ=255)                                                                                                                 aTGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGTGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                <  1:1415986/139‑1 (MQ=255)                                                                                                                   gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGaa                                                                                                              >  2:943314/1‑43 (MQ=255)                                                                                                                   gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGaa                                                                                                              <  1:943314/43‑1 (MQ=255)                                                                                                                   gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgaga              >  1:1067972/1‑139 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTAt                                                                                              <  2:1489647/54‑1 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTAt                                                                                              >  1:1489647/1‑54 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:638117/76‑1 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  2:638117/1‑76 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                        <  1:680595/124‑1 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                        >  2:680595/1‑124 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtct         >  2:758782/1‑139 (MQ=255)                                                                                                                        ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtct         >  2:848866/1‑139 (MQ=255)                                                                                                                          aaTCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTc                                         >  1:277942/1‑105 (MQ=255)                                                                                                                          aaTCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTc                                         <  2:277942/105‑1 (MQ=255)                                                                                                                               gTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc  <  2:1067972/139‑1 (MQ=255)                                                                                                                                tGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                       >  2:596297/1‑117 (MQ=255)                                                                                                                                tGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                       <  1:596297/117‑1 (MQ=255)                                                                                                                                         ||                                                                                                                                  GCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NC_000913/1216859‑1217125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑