Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,763,987	Δ1 bp	coding (23/1488 nt)	sufB ←	component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,763,987	0	G	.	100.0%	128.6 / NA	32	coding (23/1488 nt)	sufB	component of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/16);  total (16/16)

GTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCG  >  NC_000913/1763893‑1764111                                                                                               |                                                                                                                             gTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCt                                                                                 >  2:888930/1‑139 (MQ=255)      tCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                            >  1:310292/1‑139 (MQ=255)                cAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagccaca                                                                  <  2:310292/139‑1 (MQ=255)                       tCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                           <  1:888930/139‑1 (MQ=255)                       tCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                           >  2:885137/1‑139 (MQ=255)                       tCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                           >  1:1192113/1‑139 (MQ=255)                         ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                         <  2:1192113/139‑1 (MQ=255)                         ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                         <  1:885137/139‑1 (MQ=255)                                aaCTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                      >  2:1120078/1‑119 (MQ=255)                                aaCTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                      <  1:1120078/119‑1 (MQ=255)                                  cTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTa                                                                                                                <  2:299162/75‑1 (MQ=255)                                  cTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTa                                                                                                                >  1:299162/1‑75 (MQ=255)                                     ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                          >  2:1160662/1‑110 (MQ=255)                                     ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                          <  1:1160662/110‑1 (MQ=255)                                     ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                             >  1:470087/1‑139 (MQ=255)                                        gaagaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTtgtg                                          >  1:153613/1‑139 (MQ=255)                                                 ttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                  >  1:283259/1‑138 (MQ=255)                                                 ttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                  <  2:283259/138‑1 (MQ=255)                                                  tcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                <  2:153613/139‑1 (MQ=255)                                                         aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                          >  1:886849/1‑106 (MQ=255)                                                         aaTTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTAGCCACAGCCACATTCATTCt                                                          <  2:886849/106‑1 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACa                                                                                                         <  1:197533/52‑1 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACa                                                                                                         >  2:197533/1‑52 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                          >  2:1274787/1‑99 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                          <  1:1274787/99‑1 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                             >  2:220441/1‑128 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                             <  1:220441/128‑1 (MQ=255)                                                                     cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTg                                   >  2:799355/1‑117 (MQ=255)                                                                     cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTg                                   <  1:799355/117‑1 (MQ=255)                                                                                gTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCg  <  1:406336/139‑1 (MQ=255)                                                                                     gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                             <  2:1264768/107‑1 (MQ=255)                                                                                     gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATc                             >  1:1264768/1‑107 (MQ=255)                                                                                               |                                                                                                                             GTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCG  >  NC_000913/1763893‑1764111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑