Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 178.9 / NA 44coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (21/23);  total (21/23)

TGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTG  >  NC_000913/1874927‑1875171
                                                                                                                            |                                                                                                                        
tGAAGCGCCATAATCAGGTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAgg                                                                                                           >  1:943652/1‑139 (MQ=255)
 gAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGc                                                                                                          >  2:753351/1‑139 (MQ=255)
       ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCtt                                                                                                      >  1:1092583/1‑137 (MQ=255)
       ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCtt                                                                                                      <  2:1092583/137‑1 (MQ=255)
           aaTCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                                <  1:853059/139‑1 (MQ=255)
             tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              <  2:943652/139‑1 (MQ=255)
             tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              <  2:189782/139‑1 (MQ=255)
                 cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCATTGCCCCCACTCGACAGGCCCTTTCGCCATCAtct                                                                                          >  2:116625/1‑139 (MQ=255)
                         gctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAGAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATc                                                                                  >  2:1082175/1‑139 (MQ=255)
                            gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                               <  1:623669/139‑1 (MQ=255)
                             aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                              <  1:1082175/139‑1 (MQ=255)
                             aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                              <  1:116625/139‑1 (MQ=255)
                                       cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                                                    <  1:753351/139‑1 (MQ=255)
                                           aaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                              <  1:1469906/125‑1 (MQ=255)
                                           aaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                              >  2:1469906/1‑125 (MQ=255)
                                                     cGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAg                                                                                                            <  1:885227/85‑1 (MQ=255)
                                                     cGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              >  2:1009435/1‑99 (MQ=255)
                                                     cGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg                                                      <  2:913788/139‑1 (MQ=255)
                                                     cGTCTCTTTTACTATCAGTCAGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAg                                                                                                            >  2:885227/1‑85 (MQ=255)
                                                     agTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCATCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              <  1:1009435/98‑1 (MQ=255)
                                                                    cAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                     <  2:952139/62‑2 (MQ=255)
                                                                    cAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                     >  1:952139/1‑61 (MQ=255)
                                                                     aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                                                    <  1:1279135/125‑1 (MQ=255)
                                                                     aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                                                    >  2:1279135/1‑125 (MQ=255)
                                                                       tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcc                                                                                                                         >  1:1363829/1‑55 (MQ=255)
                                                                       tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcc                                                                                                                         <  2:1363829/55‑1 (MQ=255)
                                                                       tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGGAACGGGCAGGTGCAGCt                                          >  2:390260/1‑133 (MQ=255)
                                                                       tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                          <  1:390260/133‑1 (MQ=255)
                                                                                  tGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              >  1:202952/1‑70 (MQ=255)
                                                                                  tGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              <  2:202952/70‑1 (MQ=255)
                                                                                    cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                 >  1:591627/1‑65 (MQ=255)
                                                                                    cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                 <  2:591627/65‑1 (MQ=255)
                                                                                    cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                               <  2:1510938/83‑1 (MQ=255)
                                                                                    cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                               >  1:1510938/1‑83 (MQ=255)
                                                                                    cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                       >  1:483142/1‑139 (MQ=255)
                                                                                     gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAggg                      >  2:1367265/1‑139 (MQ=255)
                                                                                         aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                 <  1:1349350/60‑1 (MQ=255)
                                                                                         aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                 >  2:1349350/1‑60 (MQ=255)
                                                                                              tCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                    <  1:632808/116‑1 (MQ=255)
                                                                                              tCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                    >  2:632808/1‑116 (MQ=255)
                                                                                                 cAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              >  2:186440/1‑55 (MQ=255)
                                                                                                 cAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              <  1:186440/55‑1 (MQ=255)
                                                                                                      aaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc     <  2:483142/139‑1 (MQ=255)
                                                                                                       agccagccCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              >  2:549101/1‑49 (MQ=39)
                                                                                                       agccagccCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                              <  1:549101/49‑1 (MQ=39)
                                                                                                         ccagccCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTg  >  1:1207987/1‑139 (MQ=255)
                                                                                                             cccATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                 <  1:885317/40‑1 (MQ=38)
                                                                                                             cccATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                                 >  2:885317/1‑40 (MQ=38)
                                                                                                                            |                                                                                                                        
TGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTG  >  NC_000913/1874927‑1875171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: