Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,769,648 (C)6→5 intergenic (+162/‑55) yfjQ → / → yfjR CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,769,6430C.100.0% 141.4 / NA 36intergenic (+157/‑60)yfjQ/yfjRCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (16/20);  total (16/20)

TGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGG  >  NC_000913/2769506‑2769744
                                                                                                                                         |                                                                                                     
tgttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcc                                                                                                      <  2:1419073/139‑1 (MQ=255)
 gttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccc                                                                                                     <  1:50607/139‑1 (MQ=255)
  ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    <  2:1354201/139‑1 (MQ=255)
      tatCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccctct                                                                                                <  1:96988/139‑4 (MQ=255)
       atCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccccctctt                                                                                               >  2:286802/1‑135 (MQ=255)
         cGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTa                                                                                            <  1:574724/139‑1 (MQ=255)
            acacCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAg                                                                                         >  2:1346049/1‑139 (MQ=255)
                caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAAt                                                                                     <  2:1138149/139‑1 (MQ=255)
                 accTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATc                                                                                    >  2:1050608/1‑139 (MQ=255)
                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATcaca                                                                                 <  2:50429/137‑1 (MQ=255)
                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATcaca                                                                                 >  1:50429/1‑137 (MQ=255)
                          cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCAttt                                                                           >  1:929918/1‑139 (MQ=255)
                            tCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTtc                                                                         <  1:741903/139‑1 (MQ=255)
                             cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTtca                                                                        <  2:929918/139‑1 (MQ=255)
                                    tgCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGtt                                                                 >  2:157473/1‑139 (MQ=255)
                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTTTCCGccc                                                                                                     >  1:1458463/1‑101 (MQ=255)
                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                               <  1:1106867/138‑1 (MQ=255)
                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                               >  2:1106867/1‑138 (MQ=255)
                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGACTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTTTCCGccc                                                                                                     <  2:1458463/101‑1 (MQ=255)
                                         gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAAt                                                                                     >  2:571429/1‑114 (MQ=255)
                                         gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAAt                                                                                     <  1:571429/114‑1 (MQ=255)
                                                   aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    >  2:1504023/1‑90 (MQ=255)
                                                   aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    <  1:1504023/90‑1 (MQ=255)
                                                   aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                  <  1:1050608/139‑1 (MQ=255)
                                                   aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                  <  2:1462370/139‑1 (MQ=255)
                                                    tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAt                                                 <  1:286802/139‑1 (MQ=255)
                                                                aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                               >  1:447024/1‑113 (MQ=255)
                                                                aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                               <  2:447024/113‑1 (MQ=255)
                                                                      tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAg                               >  1:69622/1‑139 (MQ=255)
                                                                             cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                        >  2:1439765/1‑139 (MQ=255)
                                                                               ttACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    <  2:1139865/62‑1 (MQ=255)
                                                                               ttACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    >  1:1139865/1‑62 (MQ=255)
                                                                                    tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTtc                                                                         >  2:1343513/1‑83 (MQ=255)
                                                                                    tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTtc                                                                         <  1:1343513/83‑1 (MQ=255)
                                                                                    tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAc                 <  1:157473/139‑1 (MQ=255)
                                                                                      tGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCg               >  1:243212/1‑139 (MQ=255)
                                                                                       ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                >  1:148107/1‑121 (MQ=255)
                                                                                       ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                <  2:148107/121‑1 (MQ=255)
                                                                                        gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggc             <  1:1439765/139‑1 (MQ=255)
                                                                                         cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    >  1:1317198/1‑52 (MQ=255)
                                                                                         cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGcccc                                                                                                    <  2:1317198/52‑1 (MQ=255)
                                                                                         cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccc                                                                                                     <  2:256734/51‑1 (MQ=255)
                                                                                         cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGccc                                                                                                     >  1:256734/1‑51 (MQ=255)
                                                                                            ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctct                                             <  1:833738/103‑1 (MQ=255)
                                                                                            ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctct                                             >  2:833738/1‑103 (MQ=255)
                                                                                                   aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg  <  1:1346049/139‑1 (MQ=255)
                                                                                                   aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg  <  1:497644/139‑1 (MQ=255)
                                                                                                            tAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTaaa                                                                                          <  2:1457772/42‑1 (MQ=17)
                                                                                                            tAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCTCTTTaaa                                                                                          >  1:1457772/1‑42 (MQ=17)
                                                                                                                                    gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgt      >  2:1373471/1‑102 (MQ=255)
                                                                                                                                    gTCCGCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgt      <  1:1373471/102‑1 (MQ=255)
                                                                                                                                         |                                                                                                     
TGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGG  >  NC_000913/2769506‑2769744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: