Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,854,733 (T)5→4 coding (396/2079 nt) fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,854,7290T.100.0% 94.2 / NA 24coding (392/2079 nt)fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/12);  total (12/12)

TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCG  >  NC_000913/2854597‑2854846
                                                                                                                                    |                                                                                                                     
ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttggt                                                                                                                 <  2:1126235/139‑4 (MQ=255)
         gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGaa                                                                                                       <  2:1159506/139‑1 (MQ=255)
                     tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                           >  2:684301/1‑139 (MQ=255)
                                 aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCtgt                                                                                                          >  1:186053/1‑112 (MQ=255)
                                 aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCtgt                                                                                                          <  2:186053/112‑1 (MQ=255)
                                   gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                             <  2:683344/139‑1 (MQ=255)
                                             gtgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaaa                                                                   <  1:373962/139‑1 (MQ=255)
                                                   ccTGGCCGCAGCTGGACGCAGGTGGGCTCTACCCAAAATTTGGTCACTAATGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAAtt                                                                                                     >  2:975736/1‑99 (MQ=255)
                                                   ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGCAAGGGCATATTTTGGTGGCTGTGAAtt                                                                                                     <  1:975736/99‑1 (MQ=255)
                                                      ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                          >  1:1218873/1‑139 (MQ=255)
                                                       gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                         <  2:1218873/139‑1 (MQ=255)
                                                        ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTGATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                        <  2:1066180/139‑1 (MQ=255)
                                                            aGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTg                                                    >  2:1439313/1‑139 (MQ=255)
                                                             gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc                                                   >  1:1361795/1‑139 (MQ=255)
                                                                                taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAGTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                >  1:1130695/1‑139 (MQ=255)
                                                                                           tGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCa                     <  1:1439313/139‑1 (MQ=255)
                                                                                            ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa                                                                     >  1:669348/1‑90 (MQ=255)
                                                                                            ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa                                                                     <  2:669348/90‑1 (MQ=255)
                                                                                            ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                    <  1:684301/139‑1 (MQ=255)
                                                                                                       ccTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                     <  1:257044/63‑1 (MQ=255)
                                                                                                       ccTGATGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                     >  2:257044/1‑63 (MQ=255)
                                                                                                           aTGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAATGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCagag     >  2:1079146/1‑139 (MQ=255)
                                                                                                            tGCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc    >  2:1057023/1‑139 (MQ=255)
                                                                                                             gCCACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc   <  2:1361795/139‑1 (MQ=255)
                                                                                                              ccACTGGCGGCGGAAGGGCATATTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg  >  1:386756/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                     
TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCG  >  NC_000913/2854597‑2854846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: