Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,006 (C)6→5 intergenic (‑82/+27) nlpI ← / ← pnp lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,0010C.100.0% 171.2 / NA 42intergenic (‑77/+32)nlpI/pnplipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (22/20);  total (22/20)

TGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAA  >  NC_000913/3308867‑3309133
                                                                                                                                      |                                                                                                                                    
tacATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGccccc                                                                                                                                  >  2:498551/3‑139 (MQ=255)
    tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccg                                                                                                                              >  2:360004/1‑135 (MQ=255)
       tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGtccc                                                                                                                                   >  1:976170/1‑131 (MQ=255)
       tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                   <  2:976170/131‑1 (MQ=255)
                      tGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGc                                                                                                           >  2:1272586/1‑139 (MQ=255)
                                        gcgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGtt                                                                                         >  1:806983/1‑139 (MQ=255)
                                         cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                                        >  2:813238/1‑139 (MQ=255)
                                          gcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTca                                                                                       >  2:1071216/1‑139 (MQ=255)
                                              aaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGc                                                                                                                                      <  2:53408/89‑1 (MQ=255)
                                              aaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGc                                                                                                                                      >  1:53408/1‑89 (MQ=255)
                                                 aGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                                <  1:1071216/139‑1 (MQ=255)
                                                          ttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGTCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                   <  2:343558/80‑1 (MQ=255)
                                                          ttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccc                                                                                                                                   >  1:343558/1‑80 (MQ=255)
                                                           tCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCgg                                                                      <  2:806983/139‑1 (MQ=255)
                                                             ccACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtg                                                                    >  1:1423044/1‑139 (MQ=255)
                                                                 tCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                              <  1:1160271/109‑1 (MQ=255)
                                                                 tCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                              >  2:1160271/1‑109 (MQ=255)
                                                                   ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                              <  2:1423044/139‑1 (MQ=255)
                                                                             cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcc                                                                                                                                     <  2:1289905/59‑1 (MQ=255)
                                                                             cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcc                                                                                                                                     >  1:1289905/1‑59 (MQ=255)
                                                                             cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                   <  2:279370/92‑1 (MQ=255)
                                                                             cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                   >  1:279370/1‑92 (MQ=255)
                                                                             cGGTTGAATGAACGTCCTGATCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                    >  1:1006231/1‑139 (MQ=255)
                                                                                   aatgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                              <  1:1442914/91‑1 (MQ=255)
                                                                                   aatgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                              >  2:1442914/1‑91 (MQ=255)
                                                                                            ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgct                                                                  >  2:1477204/1‑110 (MQ=255)
                                                                                            ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgct                                                                  <  1:1477204/110‑1 (MQ=255)
                                                                                                ttCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGATCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTa                                 <  2:1069117/139‑1 (MQ=255)
                                                                                                     ggTTGCTTACAAGGCGTCCTGCCCAGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt                            >  1:920701/1‑139 (MQ=255)
                                                                                                     ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                <  1:1297578/71‑1 (MQ=255)
                                                                                                     ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                >  2:1297578/1‑71 (MQ=255)
                                                                                                     ggTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt                            >  2:585607/1‑139 (MQ=255)
                                                                                                      gTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGcc                                                                                 >  2:636768/1‑85 (MQ=255)
                                                                                                      gTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGcc                                                                                 <  1:636768/85‑1 (MQ=255)
                                                                                                       ttGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCa                          <  2:920701/139‑1 (MQ=255)
                                                                                                          cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCgg                                                                                                                       <  1:27442/43‑1 (MQ=255)
                                                                                                          cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCgg                                                                                                                       >  2:27442/1‑43 (MQ=255)
                                                                                                          cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGa                                                                                                                      <  1:199551/44‑1 (MQ=255)
                                                                                                          cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGa                                                                                                                      >  2:199551/1‑44 (MQ=255)
                                                                                                          cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                              >  1:430424/1‑68 (MQ=255)
                                                                                                          cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                                              <  2:430424/68‑1 (MQ=255)
                                                                                                               aaGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATa                  <  1:585607/139‑1 (MQ=255)
                                                                                                                 ggCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACg                >  1:768904/1‑139 (MQ=255)
                                                                                                                     tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                              >  1:1195886/1‑89 (MQ=255)
                                                                                                                     tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                              <  2:1195886/89‑1 (MQ=255)
                                                                                                                     tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCt                                               >  2:242443/1‑104 (MQ=255)
                                                                                                                     tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCt                                               <  1:242443/104‑1 (MQ=255)
                                                                                                                     tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGccc            <  1:360004/139‑1 (MQ=255)
                                                                                                                            cGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGAt     <  2:768904/139‑1 (MQ=255)
                                                                                                                               ttAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                >  2:88998/1‑45 (MQ=255)
                                                                                                                               ttAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                <  1:88998/45‑1 (MQ=255)
                                                                                                                               ttAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCaa  >  1:611115/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAA  >  NC_000913/3308867‑3309133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: