| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 97.1% | 117.8 / ‑3.3 | 34 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (17/16); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC > NC_000913/2484753‑2485021 | cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 1:1022076/139‑1 (MQ=255) cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa > 1:1490666/1‑133 (MQ=255) cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 2:1490666/133‑1 (MQ=255) cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccc > 2:383100/1‑139 (MQ=255) tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga < 1:641927/139‑5 (MQ=255) aataatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAg > 1:50980/1‑139 (MQ=255) tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑ccc < 1:148925/125‑1 (MQ=255) tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑ccc > 2:148925/1‑125 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATtca < 2:50980/139‑1 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATtca > 2:1278896/1‑139 (MQ=255) ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 2:1495492/1‑116 (MQ=255) ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 1:1495492/116‑1 (MQ=255) aaccaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCAtt > 2:306047/1‑139 (MQ=255) accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 2:377807/108‑1 (MQ=255) accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 1:377807/1‑108 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccc < 1:303363/108‑1 (MQ=255) accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccc > 2:303363/1‑108 (MQ=255) aTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:383100/139‑1 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 2:1266444/85‑1 (MQ=255) tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 1:1266444/1‑85 (MQ=255) ggCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc < 1:306047/139‑1 (MQ=255) cgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑ccc < 2:315811/84‑1 (MQ=255) cgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑ccc > 1:315811/1‑84 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc > 1:100831/1‑139 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c > 1:763560/1‑67 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 2:763560/67‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCaaa < 1:474420/101‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCaaa > 2:474420/1‑101 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg > 1:1424140/1‑103 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg < 2:1424140/103‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 1:873874/1‑139 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa > 2:892994/1‑59 (MQ=255) gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 1:892994/59‑1 (MQ=255) aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑ccc < 2:112986/58‑1 (MQ=255) aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑ccc > 1:112986/1‑58 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc < 1:1462852/102‑1 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTAc > 2:1462852/1‑102 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa > 2:95436/1‑36 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 1:95436/36‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCGCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa < 2:873874/139‑1 (MQ=255) cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAtt < 1:1278896/139‑1 (MQ=255) aaagCCAGTAAATATTGCTCGGGTAGCAAATTA‑CCGCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac < 2:285784/131‑1 (MQ=255) aaaaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac > 1:285784/1‑131 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 1:336522/1‑71 (MQ=255) aaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 2:336522/71‑1 (MQ=255) atTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg < 1:510333/135‑1 (MQ=255) atTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg > 2:510333/1‑135 (MQ=255) atTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt > 1:1151479/1‑139 (MQ=255) gCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACaaa > 1:876047/1‑78 (MQ=255) gCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACaaa < 2:876047/78‑1 (MQ=255) tCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc > 1:1124117/1‑97 (MQ=255) tCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc < 2:1124117/97‑1 (MQ=255) cGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg > 2:400449/1‑53 (MQ=255) cGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg < 1:400449/53‑1 (MQ=255) gggTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt > 1:550273/1‑125 (MQ=255) gggTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt < 2:550273/125‑1 (MQ=255) gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt < 2:51300/123‑1 (MQ=255) gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAt > 1:51300/1‑123 (MQ=255) gCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc < 2:1151479/139‑1 (MQ=255) | CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC > NC_000913/2484753‑2485021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |