Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,101,039 (C)7→8 coding (585/720 nt) yggN ← DUF2884 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,0321.C100.0% 116.1 / NA 34L198V (CTG→GTG) yggNDUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/18);  total (16/18)

AATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCT  >  NC_000913/3100895‑3101159
                                                                                                                                          |                                                                                                                                
aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                <  2:1208226/139‑1 (MQ=255)
aaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                <  2:768337/139‑1 (MQ=255)
      ggATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccc                                                                                                                          >  1:389057/1‑139 (MQ=255)
                      ccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGcaca                                                                                                           >  2:1360451/1‑139 (MQ=255)
                       cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCGAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                          <  2:889517/139‑1 (MQ=255)
                             gggCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAttacaga                                                                                                    <  2:982300/139‑6 (MQ=255)
                                   tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa                                                                                                   >  1:1076924/1‑134 (MQ=255)
                                   tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTaa                                                                                                   <  2:1076924/134‑1 (MQ=255)
                                   tGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                              >  1:1159286/1‑139 (MQ=255)
                                                  gagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCa                                                                               >  2:5091/1‑139 (MQ=255)
                                                   agTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAg                                                                              <  1:1360451/139‑1 (MQ=255)
                                                     tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                             >  2:57688/1‑106 (MQ=255)
                                                     tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGca                                                                                                             <  1:57688/106‑1 (MQ=255)
                                                     tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                      <  2:1311698/96‑5 (MQ=255)
                                                     tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                      >  1:1311698/1‑92 (MQ=255)
                                                              ggCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                <  1:1296535/77‑1 (MQ=255)
                                                              ggCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑c                                                                                                                                >  2:1296535/1‑77 (MQ=255)
                                                                cTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTcgcg                                                                 <  2:1159286/139‑1 (MQ=255)
                                                                          cTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCAt                                                       <  1:5091/139‑1 (MQ=255)
                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGGGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                  <  1:385323/131‑1 (MQ=255)
                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGGGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                  >  2:385323/1‑131 (MQ=255)
                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTc                                                                                                                   <  1:1135719/66‑1 (MQ=255)
                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTc                                                                                                                   >  2:1135719/1‑66 (MQ=255)
                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                  <  1:1048859/131‑1 (MQ=255)
                                                                                       ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                  >  2:1048859/1‑131 (MQ=255)
                                                                                         ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccc                                                                                                                           >  1:661125/1‑55 (MQ=255)
                                                                                         ggAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑cccccc                                                                                                                           <  2:661125/55‑1 (MQ=255)
                                                                                                 ttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                      >  1:352697/1‑48 (MQ=255)
                                                                                                 ttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑ccccccccagg                                                                                                                      <  2:352697/52‑5 (MQ=255)
                                                                                                   ttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATGCTGTAATGGGTTACCGCCGCGTTTCAGCACCGCTTTCGCGCGCATTTCATTAATGCTGTCCTGTAAAATTTCAcc                              <  2:389057/139‑1 (MQ=255)
                                                                                                         cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                  >  2:378714/1‑129 (MQ=255)
                                                                                                         cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                  <  1:378714/129‑1 (MQ=255)
                                                                                                                cACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg                                                                                      >  1:573948/1‑71 (MQ=255)
                                                                                                                cACTCGGTTTGGATTGAGGATTGCAGCCCCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg                                                                                      <  2:573948/71‑1 (MQ=255)
                                                                                                                       tttGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                          >  2:354071/1‑43 (MQ=255)
                                                                                                                       tttGGATTGAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACAt                                                                                                          <  1:354071/43‑1 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg                                                                                      <  2:262360/55‑1 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg                                                                                      >  1:262360/1‑55 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAg                                                                              <  2:181660/63‑1 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAg                                                                              >  1:181660/1‑63 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                  <  2:1063409/91‑1 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                  >  1:1063409/1‑91 (MQ=255)
                                                                                                                               gAGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCt  >  1:1176265/1‑139 (MQ=255)
                                                                                                                                aGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                              <  2:221503/46‑1 (MQ=255)
                                                                                                                                aGGATTGCAGC‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                              >  1:221503/1‑46 (MQ=39)
                                                                                                                                          |                                                                                                                                
AATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑‑CCCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCT  >  NC_000913/3100895‑3101159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑