Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,746	(A)8→9	coding (599/906 nt)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,738	1	.	A	100.0%	165.8 / NA	51	G203C (GGC→TGC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (29/22);  total (29/22)

CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511601‑3511869                                                                                                                                           |                                                                                                                                    cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a                                                                                                                                    >  1:42989/1‑139 (MQ=255)           tctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca                                                                                                                          <  2:42989/139‑4 (MQ=255)                      cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGAt                                                                                                                >  1:1135215/1‑139 (MQ=255)                              gTTCGTTTTCCGCCACCACCTTCCATATCACCGCAGCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGGGATCGACTCGTTCCACACCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGAt                                                                                                        <  2:207540/139‑1 (MQ=255)                                         gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  2:589030/1‑139 (MQ=255)                                                          cACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcgcg                                                                            >  2:1374742/1‑139 (MQ=255)                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  2:160162/114‑1 (MQ=255)                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  1:160162/1‑114 (MQ=255)                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                         >  1:1373568/1‑139 (MQ=255)                                                             cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                         >  2:584233/1‑139 (MQ=255)                                                                    aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                  >  1:80124/1‑139 (MQ=255)                                                                       tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                               <  1:589030/139‑1 (MQ=255)                                                                         gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                             <  1:865988/139‑1 (MQ=255)                                                                              tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:245086/1‑97 (MQ=255)                                                                              tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:245086/97‑1 (MQ=255)                                                                                    tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                  <  2:80124/139‑1 (MQ=255)                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATGACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                    >  2:1065421/1‑72 (MQ=255)                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                    <  1:1065421/72‑1 (MQ=255)                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  1:1222821/95‑1 (MQ=255)                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  2:1222821/1‑95 (MQ=255)                                                                                     gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                 >  1:1612/1‑139 (MQ=255)                                                                                        ttGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt                                                                                         <  2:269072/96‑1 (MQ=255)                                                                                        ttGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt                                                                                         >  1:269072/1‑96 (MQ=255)                                                                                                tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                    <  2:1491572/61‑1 (MQ=255)                                                                                                tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                    >  1:1491572/1‑61 (MQ=255)                                                                                                     tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                                 >  1:595105/1‑139 (MQ=255)                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCtt                                                     >  1:672737/1‑117 (MQ=255)                                                                                                       aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCtt                                                     <  2:672737/117‑1 (MQ=255)                                                                                                         aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGTCGAACGGTTATCCAGCCCTACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCTAATCAACTGGTGCTCGc                             <  2:864273/139‑1 (MQ=255)                                                                                                              agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:1422373/1‑65 (MQ=255)                                                                                                              agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:1422373/65‑1 (MQ=255)                                                                                                              agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  1:1489790/1‑70 (MQ=255)                                                                                                              agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  2:1489790/70‑1 (MQ=255)                                                                                                                aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                      >  1:1421495/1‑139 (MQ=255)                                                                                                                aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGCCAAATCAACTGGTGCTCGCCAACGTa                      >  2:425437/1‑139 (MQ=255)                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  1:8899/78‑1 (MQ=255)                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              >  2:8899/1‑78 (MQ=255)                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                  >  1:789306/1‑106 (MQ=255)                                                                                                                     cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                  <  2:789306/106‑1 (MQ=255)                                                                                                                      tCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  1:795249/126‑1 (MQ=255)                                                                                                                      tCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             >  2:795249/1‑126 (MQ=255)                                                                                                                      tCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGCCAAATCAACTGGTGCTCGCCAACGTAGGtgtg                <  1:425437/139‑1 (MQ=255)                                                                                                                              caTCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               <  1:1275114/100‑1 (MQ=255)                                                                                                                              caTCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               >  2:1275114/1‑100 (MQ=255)                                                                                                                               aTCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  2:395153/1‑53 (MQ=255)                                                                                                                               aTCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  1:395153/53‑1 (MQ=255)                                                                                                                                   cTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  1:1118291/49‑1 (MQ=39)                                                                                                                                   cTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             >  2:1118291/1‑49 (MQ=39)                                                                                                                                   cTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc   >  1:1044414/1‑139 (MQ=255)                                                                                                                                    tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  >  1:422029/1‑107 (MQ=255)                                                                                                                                    tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  <  2:422029/107‑1 (MQ=255)                                                                                                                                    tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  1:141406/1‑139 (MQ=255)                                                                                                                                    tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  2:90348/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                    CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511601‑3511869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑