| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 544,754 | C→T | R180H (CGT→CAT) | allC ← | allantoate amidohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 544,754 | 0 | C | T | 95.3% | 124.5 / ‑1.5 | 43 | R180H (CGT→CAT) | allC | allantoate amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (22/19); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCC > NC_000913/544618‑544885 | gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAtgc > 2:293736/1‑139 (MQ=255) ttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCgg < 1:76993/139‑1 (MQ=255) cAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCg < 1:293736/139‑1 (MQ=255) ccGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAag > 1:886423/1‑139 (MQ=255) cAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcat < 1:573292/139‑1 (MQ=255) caccacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa > 1:900455/1‑139 (MQ=255) cacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAtgc < 1:219292/82‑1 (MQ=255) cacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAtgc > 2:219292/1‑82 (MQ=255) cGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTggg > 1:840445/1‑92 (MQ=255) cGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTggg < 2:840445/92‑1 (MQ=255) cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCa > 1:843769/1‑139 (MQ=255) tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCtt > 2:715809/1‑124 (MQ=255) tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCtt < 1:715809/124‑1 (MQ=255) cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCgg < 2:1395593/72‑1 (MQ=255) cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCgg > 1:1395593/1‑72 (MQ=255) cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAACCTATTTCCTTTGGCATCACAGATATTCCGCAc > 2:387954/1‑139 (MQ=255) aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCgg < 2:337714/49‑1 (MQ=255) aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCgg > 1:337714/1‑49 (MQ=255) aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTaaa < 2:1103420/76‑1 (MQ=255) aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTaaa > 1:1103420/1‑76 (MQ=255) aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtc > 2:981553/1‑139 (MQ=255) tGTTCAATATGCAGTTCAACAAAGGCTTTGATATCCTGATGCGGAGTTAGTGGGGCGTTc > 1:869787/1‑60 (MQ=255) tGTTCAATATGCAGCTCAACAAAGGCTTTGATATCCTGATGCGGAGTTAGTGGGGCGTTc < 2:869787/60‑1 (MQ=255) ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa > 1:553108/1‑100 (MQ=255) ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa < 2:553108/100‑1 (MQ=255) tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTCAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTGCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc > 2:949314/1‑139 (MQ=255) tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc > 2:826362/1‑139 (MQ=255) aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc > 1:1086266/1‑139 (MQ=255) gTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc > 1:472534/1‑139 (MQ=255) aaGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAatat > 1:1152736/1‑139 (MQ=255) gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt < 1:805580/91‑1 (MQ=255) gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt > 2:805580/1‑91 (MQ=255) gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa < 1:866618/106‑1 (MQ=255) gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa > 2:866618/1‑106 (MQ=255) gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtttt > 1:30828/1‑139 (MQ=255) gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtttt > 2:662608/1‑139 (MQ=255) ttAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCTAGCCCAAAAATATTTTTAc < 1:387954/139‑1 (MQ=255) ttAATATCCTGATGCGGAGTCAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc < 1:949314/139‑1 (MQ=255) aaTATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg < 2:30828/139‑1 (MQ=255) atCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGccc < 2:1086266/139‑1 (MQ=255) ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa < 2:1006841/43‑1 (MQ=255) ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa > 1:1006841/1‑43 (MQ=255) ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTc < 2:566992/50‑1 (MQ=255) ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTc > 1:566992/1‑50 (MQ=255) cTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGc < 2:536981/45‑1 (MQ=255) cTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGc > 1:536981/1‑45 (MQ=255) | GTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCC > NC_000913/544618‑544885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |