Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	879,857	G→A	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI → gstB ←	soluble aldose sugar dehydrogenase glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	879,857	0	G	A	100.0%	142.1 / NA	45	*372* (TAG→TAA)  S208S (AGC→AGT)	yliI gstB	soluble aldose sugar dehydrogenase glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/21);  total (24/21)

ACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAGC  >  NC_000913/879726‑879988                                                                                                                                    |                                                                                                                                    aCAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACg                                                                                                                               <  2:796306/138‑1 (MQ=255) aCAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACg                                                                                                                               >  1:796306/1‑138 (MQ=255)   agaagaTGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                            <  2:620884/139‑1 (MQ=255)             cGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATcacca                                                                                                                  >  2:1284904/1‑139 (MQ=255)                     aaCGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcgc                                                                                                          <  1:1284904/139‑1 (MQ=255)                       cGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                           >  2:463617/1‑136 (MQ=255)                       cGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                           <  1:463617/136‑1 (MQ=255)                          cagaagGGCA‑CGGATTCGCGATGGTCGCACTGGACCCGAACGTTATTTATTCGTTATCACCGACGAGTTCAATGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCg                                                                                                    <  2:371094/135‑1 (MQ=255)                            agagGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgg                                                                                                   >  1:1362665/1‑139 (MQ=255)                              agGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCgggg                                                                                                 >  2:923218/1‑139 (MQ=255)                                ggCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                               >  2:1312859/1‑139 (MQ=255)                                          tCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                            <  1:1306896/100‑1 (MQ=255)                                          tCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                            >  2:1306896/1‑100 (MQ=255)                                                                     ttatttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGa                                                          >  1:1095760/1‑139 (MQ=255)                                                                         ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                      <  1:923218/139‑1 (MQ=255)                                                                         ttatACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                      <  2:1362665/139‑1 (MQ=255)                                                                                  ctcACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAgg                                             >  2:597152/1‑139 (MQ=255)                                                                                       cgacgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCt                                                                                 >  2:384440/1‑98 (MQ=255)                                                                                       cgacgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCt                                                                                 <  1:384440/98‑1 (MQ=255)                                                                                         acgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGcc                                      >  1:816700/1‑139 (MQ=255)                                                                                         acgaGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGcc                                      <  1:1312859/139‑1 (MQ=255)                                                                                                 aGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTaa                              <  2:816700/139‑1 (MQ=255)                                                                                                   tGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTc                                                 >  2:621260/1‑118 (MQ=255)                                                                                                   tGGGGAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTc                                                 <  1:621260/118‑1 (MQ=255)                                                                                                     gggAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                            >  1:449601/1‑41 (MQ=255)                                                                                                     gggAATTACTTAAAGTTAGCCCACGCAATTAACTAACGGGa                                                                                                                            <  2:449601/41‑1 (MQ=255)                                                                                                        aaTTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                      >  2:1113708/1‑108 (MQ=255)                                                                                                        aaTTACTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcgcg                                                      <  1:1113708/108‑1 (MQ=255)                                                                                                             cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                           <  1:417018/50‑1 (MQ=255)                                                                                                             cTTAAAGTTAGCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                           >  2:417018/1‑50 (MQ=255)                                                                                                                       gCCCACGCAATTAACTAACTGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcg                                                        <  2:865673/91‑1 (MQ=255)                                                                                                                       gCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                           <  2:880094/40‑1 (MQ=39)                                                                                                                       gCCCACGCAATTAACTAACGGGAATCATCACCACTTTgcg                                                                                                           >  1:880094/1‑40 (MQ=255)                                                                                                                       gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                         <  2:1132749/58‑1 (MQ=255)                                                                                                                       gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGt                                                                                         >  1:1132749/1‑58 (MQ=255)                                                                                                                       gCCCACGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGAcg                                                        >  1:865673/1‑91 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                               <  2:1087419/47‑1 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCg                                                                                               >  1:1087419/1‑47 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCa                                                                                           >  1:1335574/1‑51 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCa                                                                                           <  2:1335574/51‑1 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatag   >  2:788957/1‑139 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgata    >  2:450885/1‑138 (MQ=255)                                                                                                                            cGCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgata    <  1:450885/138‑1 (MQ=255)                                                                                                                             gCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatagc  >  2:394803/1‑139 (MQ=255)                                                                                                                             gCAATTAACTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTgcgatagc  >  2:166709/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                    ACAGAAGATGGCCGTATTTTAACGGACAGAGGGCAGCGAATTCGTGATGTTCGCACTGGACCCGACGGTTATTTATACGTTCTCACCGACGAGTCCAGTGGGGAATTACTTAAAGTTAGCCCACGCAATTAGCTAACGGGAATCATCACCACTTTGCGCACTGCGGGGCGTTCAGTGAGTTGCTGATACCAGCGTTGCAGATTTGGACGCGGTGTCCAGGTCAGGCCAACGTTAAACAAATTGTAGATGAACGGTGCGATAGC  >  NC_000913/879726‑879988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑